简介:BambooOSB(BOSB)mediumexperimentallineandkeyequipmentsarepresentedforthefirsttimebasedontheanalysisofBOSBproductiontechnologiesandprocessesrequirements.ComponentofBOSBmediumexperimentallineandkeyequipmentsstated.ThepaperillustratedBOSBflaker,glueblender,orientingformer.Thenmediumexperimentallineisgiventoagreatdealoftestrunandoptimization.ThetestsindicatedthatthemediumexperimentallineoperatedsmoothlyforproducingBOSB.TestedBOSBkeyperformanceindicatorswerehigherthantheLY/T1580-2000(OSB/4).
简介:Toobtainasuitableconditionforelectroporationtransformationinindicarice,the10-day-oldimmatureembryoswereselectedforoptimizationexperiments.Theresultsshowedthatonepulseat850V/cm,950μFcapacitance,200μLelectroporationbufferwith70mmol/Lsodiumglutamate,100μg/mLplasmid,50μg/mLcarrierDNA,20embryospercuvette,0°CtreatmentandCCmediumwerethebestparameters,whichnotonlyimprovedthetransformationefficiencyto30.89%,butalsoamelioratedtheembryosurvivalratioto95.92%.Tofurtherverifythepracticabilityofthiscondition,theembryosfromanotherindicaricevarietyandaricetypeIImetallothionein-likegene(OsMT2bL)promoter::mgfp5::gusAconstructweretested,andspecificGUSexpressionontheembryoswasvisualizedbyhistochemicalstaining.TheresultsshowedthattheGUSexpressionontheembryosactivatedbytheOsMT2bLpromoterwasmainlyconcentratedontheapicalpointoftheplumulewhereastheexpressiondrivenbyCaMV35Spromoterwasdistributedonnearlyallareasoftheelectroporatedtissues.Theseresultsindicatedthattheoptimizedembryoelectroporationconditionscouldbeusednotonlyingenetictransformationofindicaricebutalsoinassayofgeneregulationonembryos.
简介:AllfactorsaffectingthePCRsysteminPinusmassonianaLambwereinvestigatedonebyone.TheresultsshowthattheoptimumPCRsystemofEST-SSRwere:2μL10×Buffer(Mg2+Free),30ngtemplateDNA,0.1875mmol/LdNTPs,3.75mmol/LMg2+,8pmolprimerpair,1.0UTaqDNApoly-meraseintotal20μLreactionsystem.Finally,atotalof11isolatesofP.massonianawasusedfortestingthestabilityofthePCRamplification.TheresultsprovethattheoptimumPCRsystemwasstable,reliable,highlyrepetitive.
简介:AccordingtotheunificationprincipleofsystemstructureandsystemfunctionoftheWaterResourceConservationForests,sevenfactorswereselectedfromstandspatialstructure,treesstructureandhealthyofwoods,themultifunctionmanagementoptimizationmodeltargetwasconfirmedbyusingnonlinearitymulti-objectiveprogrammingapproach,andthetargetfunction-standspatialstructurehomogeneityindexwasdefinedtoestablishspatialoptimizationmodelswithrestrainingconditionssetupindiversityofstandstructureandspatialstructure.ThespatialstructureofavailabletypicalstandinthewetlandareainsouthernDontingLakewasoptimizedbymeansofselectivecuttingandreinforcementplanting.Theresultsshowedthatthespatialstructurewasimprovedobviouslyandtreesdiversityandstandhealthwerenotweakened.It’ssuggestedthattheestablishedoptimizationmodelcaneffectivelyguidemultifunctionmanagement,standstructureoptimizationandthesteadinessofecologicalecosystemanditisconvenientandoperableinpractice,andit’sveryimportanttoprotectandrecoverecosystemofWaterResourceConservationForests.