简介:ThedifferentiationprocessincludingsomaticembryogenesisindifferentGinkgoexplantsinvitroculturewerestudiedbycytologicalobservation.Theresultsareasfollows:1)twocompletecotyledonsandaembryobudwereobservedinmatureembryosandseveralsecretoryacavitivesappearedinmaturationregionofembryobuds,hypocotyls,cotyledonsandradiclesafterculturing20days;twoincompletecotyledonsandaembryobudprimordiawerefoundinlargecotyledonembryos.Theproembryooftwocells,fourcells,multi-cellular,andglobularembroyweredevelopedfromthecallusofthesmallcotyledonembryos.2)Thedifferentiationofcotyledonexplantsstartedfromepidermalcells,andgraduallyformedmeristematiccellmassinthecorticalcells,andeventuallyadventitiousbudswereobserved.3)TheadventitiousrootsofGinkgooriginatedinthecellsatthecrossofvascularcambiumandvascularrays.4)Thetypeofrootingbelongstoinductiontypebyrootprimordium.Theformedadventitiousrootswereobservedafter20days.