简介：米饭heading-date-related异种从60Co被孤立--Zhejing22的ray-induced变化水池，在浙江省的常规装饰用的梨树栽培变种，中国。异种被几乎20d的一个推迟的标题日期比野类型植物长描绘。基因分析表明变化被一个单个原子编码控制作为HD(t)被设计的后退的基因(标题日期尝试性地)。孤立HD(t)基因，一条基于地图的克隆途径用植物从在hd(t)异种(装饰用的梨树)之间的十字导出的479F2变异的个人被采用？？

简介：三个米饭变化，Zhonghan3，Shanyou63和Aizizhan，当在检测微分活跃甲基的材料骑车并且响应干旱应力转移相关基因表示，被使用。实验被在干旱的条件下面的微分显示器技术与10%PEG6000答案模仿了的基因薄片和mRNA执行。结果显示甲基周期能在Zhonghan3和Shanyou63的叶子被激活，但是在干旱应力下面在Aizizhan的叶子禁止了。而且，干旱应力能导致很多methyltransferase基因的表示，特别Rubisco蛋白质methylation的抄写联系了基因，它是有益的因为Rubisco蛋白质氧化和降级的预防，和干旱应力能禁止DNAmethyltransferase基因和histonemethyltransferase基因的抄写。这结果证实活跃甲基骑车并且转移相关基因涉及米饭干旱抵抗。

简介：thermo感觉迟钝的苍白的绿叶异种(pgl2)从T-DNA被孤立米饭的插入的转基因的线(OryzasativaL。subsp。装饰用的梨树cv。Nipponbare)。基因分析显示显型被一个后退的变化在单个原子编码基因引起。印射PGL2基因，一张F2人口被与Longtefu穿过异种构造(OryzasativaL。subsp。indica)。PGL2地点粗略地在染色体8上被连接到SSR标记RM331。细微地印射基因，14个新InDel标记在标记，和PGL2附近被开发进一步被印射到2.37Mbcentromeric区域。叶子的叶绿素内容上的分析证明在全部的叶绿素(Chl)异种和野类型之间没有明显的差别当在异种的Chla/Chlb的比率仅仅是大约1时，满足，它在野类型是比那显然低的，建议PGL2基因与在Chl之间的变换有关是andChlb。而且，在centromeric区域附近的教材设计的方法被讨论，它将提供卓见进在工厂着丝点的功能的基因的好印射。

简介：六高并且从dominantsemi矮子异种(Y98149)导出的矮子near-isogenic线被选择学习高度表示和敏感到gibberellicacid(GA_3)。长度第4-5内部节点，第三，第二，1st内部分别地，从顶的节点和在isogenic附近的六个矮子的圆锥花序长度是在六高的97.2%那些，53.3%，65.1%，61.9%和94.7%显示主导的相形见绌半的基因显著地禁止了内部节点延伸。而且，Y98149(变异的类型)比Y98148(野生型)对GA_3更敏感，并且在植物有更低的GA_3集中，大约78%Y98148。

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简介：ThecurrentstatusofchemicalfertilizersproductionandconsumptioninChinaaswellastheirimportantrolesinChinesemodernagriculturearediscussedwithspecialconcernstotheenvironmentalissuesrelatedtochemicalfertilizeruse.Ontheonehand.thetotalamountofchemicalfertilizerproducedisinsufficienttomeettheagriculturalneeds.Ontheotherhand.theproductionandconsumptionofchemicalfertilizersinChinaareobviouslynotbalanced.Insomeareasoverapplicationofnitrogenfertilizersandlossofphosphatefertilizerduetosoilerosionhaveresultedinsomeundesirableenvironmentalproblemssuchasincreaseofnitrateinwaterandeutrophicationofwaterbodies.Maximumscientificusesoforganicmanuresincombinationwithreasonableuseofchemicalfertilizersarepartofgoodpracticesnotonlyinincreasuingsoilproductivityandkeepingsustainableagriculturedevelopmentbutalsoinminimizingtheirdetrimentaleffectsontheenvironment.

简介：Alaboratory-basedaerobicincubationwasconductedtoinvestigatenitrogen(N)isotopicfractionationrelatedtonitrificationinfiveagriculturalsoilsafterapplicationofammoniumsulfate((NH4)2SO4).Thesoilsampleswerecollectedfromasubtropicalbarrenlandsoilderivedfromgranite(RGB),threesubtropicaluplandsoilsderivedfromgranite(RQU),Quaternaryredearth(RGU),QuaternaryXiashuloess(YQU)andatemperateuplandsoilgeneratedfromalluvialdeposit(FAU).Thefivesoilsvariedinnitrificationpotential,beingintheorderofFAU>YQU>RGU>RQU>RGB.SignificantNisotopicfractionationaccompaniednitrificationofNH+4.δ15NvaluesofNH+4increasedwithenhancednitrificationovertimeinthefouruplandsoilswithNH+4addition,whilethoseofNO-3decreasedconsistentlytotheminimumandthereafterincreased.δ15NvaluesofNH+4showedasignificantlynegativelinearrelationshipwithNH+4-Nconcentration,butapositivelinearrelationshipwithNO-3-Nconcentration.TheapparentisotopicfractionationfactorcalculatedbasedonthelossofNH+4was1.036forRQU,1.022forRGU,1.016forYQU,and1.020forFAU,respectively.Zero-andfirst-orderreactionkineticsseemedtohavetheirlimitationsindescribingthenitrificationprocessaffectedbyNH+4inputinthestudiedsoils.Incontrast,Nkineticisotopefractionationwascloselyrelatedtothenitrifyingactivity,andmightserveasanalternativetoolforestimatingthenitrificationcapacityofagriculturalsoils.

简介：43个米饭变化的DNA碎片被放大，11份教材基于植物的抵抗基因类似物(RGA)设计了，并且变化的强风抵抗被接种与33识别从云南省收集的Magnaporthegrisea孤立，中国。聚类结果与0.6117的一个关联系数揭示了在强风抵抗和DNA乐队之间的重要关联(α=0.01)，显示抵抗分析基于接种与那基于聚类分析的RGA-PCR与一致。关联系数，然而，从0.1701～0.535取决于教材。五份教材，S1/AS3，S1INV/S2INV，XLRRFor/XLRR加快，Pto-Kin1IN/Pto-Kin2在，和NLRRFor/NLRR加快可能被申请在他们的乐队数字和多型性的考虑的强风抵抗鉴定，和他们有强风抵抗的关联系数是0.5305，0.4898，0.4059，0.3719和0.3524分别地。而且，除了二个高度易受影响的变化，CO39和Lijiangxintuanheigu的indica和装饰用的梨树米饭，能被11份教材很好分类。

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简介：抗菌剂肽是有抗菌剂活动的多肽。从中国虾(FenneropenaeusChinensis)的抗菌剂肽基因Np3和Np5集成于OryzasativaL。subsp。装饰用的梨树cv。由Agrobacterium的Aichiashahi调停了转变系统。PCR分析证明Np3和Np5的积极比率分别地在T0产生是36%和45%。RT-PCR分析证明抗菌剂肽基因在T1产生被表示，并且在转基因的植物和非转基因的植物之间的农学的特点地没有明显的差别。四Np3和Np5在T1产生的转基因的线与Xanthomonasoryzaepv被接种。oryzae紧张CR4，和所有四根转基因的线显著地提高了抵抗到紧张CR4引起的细菌的老家。Np5转基因的线也显示出更高的抵抗到紧张JS97-2，Zhe173和OS-225引起的细菌的老家。有Np5基因力量的转基因的线拥有宽广光谱抵抗到米饭，这被建议细菌的老家。

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简介：Afragmentwithabout798bpofcabgenewasamplifiedfromthefirststrandofMoso(Phyllostachysedulis)cDNAthroughRT-PCRmethod,namedascab-PhE4(cabgene4fromPh.edulis).Thecab-PhE4(GenBankaccessionnumber:EF405878)geneencodes265aminoacid.Thebioinformaticsanalysisindicatedthattheproteinencodedbycab-PhE4hasachlorophylla/b-bindingdomain(64th-232ndposition),aproteinkinaseCphosphorylationsite(33rd-35thposition),aN-myristoylationsite(169th-174thposition),andaninvolucrinrepeat(207th-216thposition).Theaminoacidsequenceofcab-PhE4showedhighsimilaritywiththecabgenesofZeamays,Triticumaestivum,Musaacuminata,PanaxginsengandOryzasativa,morethan90%,respectively.

简介：有扭曲的壳的异种在米饭(OryzasativaL.)的一张繁殖人口被发现。异种除了扭曲的壳显示出更少的谷物重量和劣等的谷物质量。基因分析显示异种的显型被单个后退的基因控制(暂时指定了为TWH)。印射TWH基因，一张F2人口被穿过twh异种到R725产生，有正常的壳的一个indica米饭变化。为bulkedsegregant分析，变异的植物的体积被从10扭曲壳植物混合植物织物的相等的数量准备，正常植物的体积被分享10正常壳的植物的相等的数量织物获得。207份简单顺序重复(SSR)教材，在12个米饭染色体上分布式，被用于父母和二体积的多型性分析。TWH地点开始在染色体2上近被印射到SSR标记RM526。因此，进一步印射在标记RM526附近用50份SSR教材被执行。TWH在在1.4厘米的基因距离的染色体2和2.7的长手臂上在SSR标记RM14128和RM208之间被限定厘米分别地。这些结果为进一步好的印射提供基础，克隆并且TWH基因的功能的分析。

简介：Thewaxygene(Wx)inrice,whichencodesthegranuleboundstarchsynthaseenzyme,isresponsibleforamylosesynthesis.Glutinous(sticky)ricehaslittleornoamylosethatcanbeusedinvariousapplications,suchasbrewing.Inthisstudy,knockoutoftheWxgenewithCRISPR/Cas9technologywasconductedintwoelitejaponicaricelines,Huaidao5(HD5)andSuken118(SK118),aimingtodevelopelitestickyricevarieties.WeachievedsixhomozygousT0plantswithmorethan200bpdeletionintheWxgene,aswellas36wx-HD5and18wx-SK118homozygoustransgene-freeplantsintheT1generation.Theseedsofallthemutantswerewhiteandopaque,similartothoseofstickyrice,andcontainedonly2.6%–3.2%amylose.Resultsofscanningelectronmicroscopyshowedthatthequalityofricedidnotchange.Inconclusion,wesuccessfullydevelopedtwoelitestickyricevarieties.

简介：光洁特点印射的继承分析和基因用在青春期的米饭材料之间的十字被进行Chuanxiang29B(混合米饭的一根芳香的精英维护者线)，和二光洁米饭材料，Lemont和R2(R2是混合米饭的一根新restorer线)。从二个十字的所有F1植物有青春期的叶子，证实在Chuanxiang29B的短柔毛特点在光洁特点上是主导的。在F2个人，分离比率三对一光洁植物青春期建议单个光洁基因在Lemont和R2是在场的。用bulkedsegregant分析，简单顺序重复标记的连接分析证明光洁基因，gl-1，分别地与染色体5上的0.19和0.92厘米的基因距离是由GL311和GL8的flanked。

简介：Bt5198，包含Bt基因的一根新米饭restorer线，与BtMinghui63从精英restorer线Chenghui177的十字和回交被开发，一根转基因的Btrestorer线。生来的线用PCR扩大，测试纸评估，在实验室的昆虫抵抗评估和稻地被评估，大米强风抵抗的托儿所评估和农学的特点的家谱选择。当米饭灰煤杆在实验室与有斑纹的茎borer(SSB)的鸡蛋被接种时，Bt5198和BtMinghui63上的幼虫的死亡是100%。Bt5198在地条件下面对SSB和黄茎borer(YSB)是高度抵抗的。F1混血儿源于Bt5198和四细胞质的男性无菌(厘米)线对SSB和YSB也高度抵抗并且有重要杂种优势。米饭强风抵抗的二年的评估BtMinghui63比那些更好证实到叶的Bt5198的抵抗层次爆炸，颈强风类似于Chenghui177并且显著地的那些。Bt5198的种子萌芽能力和花粉产量与Chenghui177是类似的，建议进新restorer线的Bt基因的介绍没有种子活力或种子生产的收益上的重要效果。识别Bt基因的存在，把测试纸检查与昆虫抵抗的评估相结合是有效的，在实验室并且在地条件下面。

简介：Alightbrownspotted-leafmutantofricewasisolatedfromanethanemethylsulfonate(EMS)-inducedIR64mutantbank.Themutant,designatedaslbsl1(lightbrownspotted-leaf1),displayedlightbrownspotinthewholegrowthperiodfromthefirstleaftotheflagleafundernaturalsummerfieldconditions.Agronomictraitsincludingplantheight,growthduration,numberoffilledgrainsperpanicle,seed-settingrateand1000-grainweightofthemutantweresignificantlyaffected.Geneticanalysisshowedthatthemutationwascontrolledbyasinglerecessivegene,tentativelynamedlbsl1(t),whichwasmappedtotheshortarmofchromosome6.Bydevelopingsimplesequencerepeat(SSR)markers,thegenewasfinallydelimitedtoanintervalof130kbbetweenmarkersRM586andRM588.Thelbsl1(t)geneislikelyanovelricespotted-leafgenesincenoothersimilargeneshavebeenidentifiednearthechromosomalregion.Thegeneticdataandrecombinationpopulationsprovidedwillfacilitatefurtherfine-mappingandcloningofthegene.

简介：ThecompleteopenreadingframeofOsPIN1awasamplifiedthroughreversetranscriptase-polymerasechainreaction(RT-PCR)basedonthesequencedepositedinGenBanktoexploretherelationshipbetweentheauxineffluxproteinOsPIN1aandthenegativephototropismofriceroots.SequencingresultsshowedthattheGCcontentofOsPIN1awas65.49%.ThefusionexpressionvectorpCAMBIA-1301-OsPIN1a::GFPcontainingtheOsPIN1ageneandacodinggreenfluorescentprotein(gfp)genewasconstructed.ThefusionvectorwastransferredintoonionepidermalcellsbyAgrobacteriumtumefacienstransformation.ThetransientexpressionofOsPIN1a-GFPwasmainlylocatedinthenucleusandcellmembrane.Moreover,thetransgenicplantswereobtainedbyAgrobacterium-mediatedgenetictransformation.MoleculardetectionperformedbyusingPCRandβ-glucuronidasestainingshowedthatthetargetconstructwasintegratedintothegenomeofrice.Thenegativephototropiccurvaturesofthetransgenicricerootswerehigherthanthoseofthewildtype.Similarly,theexpressionlevelsofOsPIN1ainthetransgenicplantswereconsiderablyhigherthanthoseinthewild-typeplants.TheseresultssuggestthatOsPIN1aiscrucialinthenegativephototropiccurvatureofriceroots.

简介：BydeterminationofthechangeofendogenoushormoneZr,iPA,GA3,IAAandABAduringdifferentflowerbuddifferentiationstagesofPhyllostachyspraecox,whichisidentifiedthroughbothfieldobservationandlabanalysis,andwiththereferencetothepreviousresearchachievementsonbambooflowering,thefloweringmechanismassumptionofPhyto-HormoneRegulationandGeneActivationofPh.praecoxisinducedinthisarticle:Bambooflowerbuddifferentiationcanbedividedinto3stages,i.e.flowerbudinduction,flowerbudinitiationandflowerbuddevelopment;Bambooleavessenseandreceivefloweringsignalsfromenvironmentstochangeitshormonelevel,esp.ratiosofiPA/ABAandiPA/GA3;FloweringgeneisactivatedoncetheratiosofiPA/ABAandiPA/GA3reachaproperthreshold,anditproducesDNAandRNAcarryingfloweringcodeandtransportsthemtotoporsidebudsnearby,andthenproteinnecessaryforflowerbuddifferentiationcomesout,asaresultofwhichtheflowerbudinductionistriggedandstarted,followedbyflowerbudinitiationanddevelopment.Intheinductionstage,ratioofC/Nisnearlyconstant,butincreasesintheinitiationstage.ThereforeitclarifiesthattherisingofC/Nratiodoesnotbringaboutbamboofloweringinitially,anditisafollow-upreactionsofprocessinitiationofbambooflowering.Itprovesthatbamboorhizomeisdirectlyinvolvedintheflowerbuddifferentiation.Thisassumptioncanwellexplainmysteriousphenomenaofbambooflowering,andbyintegratingthecurrentseveralassumptions,answerthedifficultandperplexingquestionsregardingbamboofloweringwhichhavenotbeenansweredbythepresentassumptions.