简介：Anagrusnilaparvataeisthedominanteggparasitoidofriceplanthoppersandplaysanimportantroleinbiologicalcontrol.Symbioticbacteriacansignificantlyinfluencethedevelopment,survival,reproductionandpopulationdifferentiationoftheirhosts.TostudytheinfluenceoftemperatureonsymbioticbacterialcompositioninthesuccessivegenerationsofA.nilaparvatae,A.nilaparvataewereraisedunderdifferentconstanttemperaturesof22°C,25°C,28°C,31°Cand34°C.Polymerasechainreaction-denaturinggradientgelelectrophoresiswasusedtoinvestigatethediversityofsymbioticbacteria.OurresultsrevealedthattheendophyticbacteriaofA.nilaparvataewerePantoeasp.,Pseudomonassp.andsomeunculturedbacteria.ThebacterialcommunitycompositioninA.nilaparvataesignificantlyvariedamongdifferenttemperaturesandgenerations,whichmightbepartiallycausedbytemperature,feedingbehaviorandthephysicalchangesofhosts.However,theanalysisofwspgeneshowedthattheWolbachiainA.nilaparvataebelongedtogroupA,sub-groupMorsandsub-groupDro.Sub-groupMorswasabsolutelydominant,andthisWolbachiacompositionremainedstableindifferenttemperaturesandgenerations,exceptforthe3rdgenerationunder34°Cduringwhichsub-groupDrobecamethedominantWolbachia.Theaboveresultssuggestthatthecontinuoushightemperatureof34°CcaninfluencetheWolbachiacommunitycompositioninA.nilaparvatae.