简介:目的通过借助经典光毒剂8-甲氧补骨酯素(8-MOP)研究UVA、UVB、UVA+UVB3种不同光源对光毒性斑贴试验结果的影响.方法豚鼠138只,健康受试者22名.光源为PhilipsUVA,PhilipsUVB,SUV1000日光模拟器.试验物为2%8-MOP.①豚鼠对8-MOP+UVA(PUVA)和8-MOP+UVB(PUVB)的反应.②豚鼠对8-MOP+UVA(PUVA)和8-MOP+UVA+UVB(PUVA+UVB)的反应.③人体对8-MOP+UVA(PUVA)和8-MOP+UVA+UVB(PUVA+UVB)的反应.结果PUVA组的反应率和单纯UVA照射组有显著差异(P<0.01),PUVB组的反应率和单纯UVB照射组的差异无显著性(P>0.05).PUVA+UVB组试验区域3d皮肤反应评分变化趋势的斜率大于PUVA组.人体试验结果同动物试验结果相一致.结论在以8-MOP为受试物的光斑贴试验中,UVB检测效果较差,而UVA和PUVA+UVB均能检测出8-MOP的光毒性,但UVA+UVB要优于UVA.因而UVA+UVB是一种理想的光斑贴试验光源.
简介:摘要目的调查慢性皮炎湿疹患者中常见的接触性变应原并探讨其与患者的性别、年龄及临床特征之间的相关性.方法对2013年7月至2014年12月在北京医院皮肤科门诊就诊的252例慢性皮炎湿疹患者进行瑞敏标准变应原系列斑贴试验,应用统计学方法比较常见变应原阳性率的高低与慢性皮炎湿疹患者的性别、年龄、病程、皮疹发生部位、个人过敏史和家族过敏史的相关性.结果252例患者斑贴试验总阳性率37.70%,变应原阳性率位居前5位的依次是硫酸镍、芳香混合物、对苯二胺基质、重铬酸钾、卡巴混合物.男性患者重铬酸钾、对苯类混合物和卡巴混合物三种变应原的阳性率高于女性患者.老年组(≥60岁)的卡巴混合物阳性率高于非老年组;非老年组的硫酸镍阳性率明显高于老年组.有个人过敏史、皮疹泛发的患者的总阳性率较高.结论慢性皮炎湿疹患者斑贴试验的总阳性率<40%;主要变应原为硫酸镍、芳香混合物、对苯二胺基质、重铬酸钾、卡巴混合物;变应原阳性率的高低与患者的性别、年龄、个人过敏史及发病部位有一定关联.关键词慢性皮炎湿疹;斑贴试验;变应原Analysisonpatchtestresultsin252patientswithchronicdermatitis/eczemaYANGMin,BaoYing-qiu,CHANGJian-min(DepartmentofDermatology,BeijingHospital,Beijing100730,China)AbstractObjectiveToinvestigatecommonallergensamongchronicdermatitis/eczemapatientsandthecorrelationbetweenthesecommonallergensandpatients’gender,age,andclinicalcharacteristics.MethodsWeusedRuiminstandardallergensseriestopatchtest252patientswithchronicdermatitis/eczemainouroutpatientdepartmentfromJuly2013toDecember2014.Correspondingstatisticalmethodswereusedtoanalyzethecorrelationbetweenthepositiveratesofcommonallergensandpatients’gender,age,durationofthedisease,locationanddistributionofthelesion,individualhistoryofallergy,andfamilyhistoryofallerGgy.ResultsTheoverallpositiverateoftestedallergensin252patientswas37.70%.Thefivemostcommonpositivetestedallergensare(fromfirsttofifth)nickelsulfate,fragrancemix,4-phenylenediaminebase,potassiumdichromate,andcarbamix.Malepatientshadhigherpositiveratesforpotassiumdichromate,paraGbensin,andcarbamixcomparedtofemalepatients.Thepatientsinelderlygroup(above60yearsold)hadhigherpositiverateforcarbamixandsignificantlylowerpositiveratefornickelsulfatecomparedtonon-elderlygroup.Thepatientswithindividualhistoryofallergyorgeneralizedrashhadhighertotalpositiverateoftestedalergensthanthosewithnohistoryofallergyornon-generalizedrash.ConclusionThetotalpositiverateofpatchtestinginchronicdermatitis/eczemapaGtientsis<40%.Themainallergensfoundinourstudyincludenickelsulfate,fragrancemix,4-phenylenediaminebase,potassiumdichromate,andcarbamix.ThepositivKeeryatweosrodfsseveralallergenswererelatedtopatients’gender,age,individualhistoryofallergy,andthedistributionofrash.chronicdermatitis/eczema;patchtest;allergens中图分类号R758文献标识码A文章编号1008-6315(2015)12-1024-02