简介:AbstractAvian influenza remains a threat to human wellbeing. Hypochlorite derivatives are commonly used as disinfectants to prevent the spread of the disease. The World Health Organization (WHO) has listed chlorine dioxide (ClO2) as an A1-level, safe, and efficient disinfectant. In this study, we tested the efficacy of ClO2, in aqueous solution and gas forms, against avian influenza A (H7N9) virus. The virus suspension was mixed with ClO2 aqueous solutions of various concentrations and for various time intervals. Aliquots of the mixture were then serially diluted, and the 50% tissue culture infective dose (TCID50) was measured with a hemagglutination test on MDCK cells. ClO2 gas produced from generators was introduced in a chamber containing the virus suspension in a Petri dish. The infective activity of the surviving virus was measured by the hemagglutination test. An aqueous solution of ClO2 at 126 µg/mL for 15 s was effective given that no surviving virus was detected with the hemagglutination test. ClO2 gas at >5 µL/L sustained for 1 h inactivated the virus effectively, while at 2.5 µL/L for 1 h, it only partially inactivated the virus. ClO2 as gas or aqueous solution at a certain concentration is effective in inactivating the H7N9 virus, and can be applied for the decontamination and disinfection of environments.
简介:目的及时了解国内外对新禽流感A(H7N9)的科研进展,把握防治禽流感A(H7N9)的主动权。方法及时收集国内外新发表的论文及报道,进行分析综合。结果初步证明了新禽流感A(H7N9)病毒的性质,基因特点,致病性,传播性,及其对药物的敏感性和防控要点。结论禽流感A(H7N9)病毒是一种三原重组的新病毒,对禽类及哺乳动物(包括人)都有致病性,且对人的毒性较强;但可防,可控,可治。目前对其认识还在随科研深入而发展,仍有不少不定因素,必须保持高度警惕,继续密切关注。
简介:摘要目的探讨人感染H7N9禽流感重症患者的护理要点,以提高护理质量。方法回顾性分析总结我院从2017年4月21日至5月19日收治的9例人感染H7N9禽流感重症病例护理经过。结果9例患者中3例给予有创机械通气和俯卧位通气,安置了气管插管、动静脉导管、尿管等,2例给予ECMO治疗;6例无创通气。未发生导管相关性感染及褥疮等;1例患者治疗过程发生严重情绪失控,经心理护理后好转。3例死亡,6例治愈出院。结论严密监测病情,做好各类导管管理及住院期间患者的心理护理,是成功抢救人感染H7N9禽流感重症患者、提高危重患者生存率的重要保障。
简介:目的建立能快速准确检测发热病人咽拭子样本中2013新型H7N9禽流感病毒的四重RT—PCR方法。方法针对甲型流感病毒的M基因设计通用引物,针对2013新型H7N9禽流感病毒的HA和NA基因设计特异性引物,选择人源看家基因beta—aetin设计质控引物,通过优化实验条件建立一步法四重RT—PCR反应体系。与商品化实时荧光RT—PCR试剂盒进行方法比对。结果成功建立四重一步法RT—PCR筛查2013新型H7N9禽流感病毒的检测技术。结论该方法简便、实用、成本低廉,适用于2013新型H7N9禽流感病毒的快速检测。
简介:摘要通过对H7N9型禽流感的病原学、流行病学特征、临床特点和我国内地H7N9型禽流感总体发病情况和疫情趋势研判,阐述了该疾病的基本情况,并提出了防控措施和应急处置流程。
简介:BackgroundAcuterespiratorydistresssyndrome(ARDS)causedbyH7N9influenzainpregnantwomanisalife-threateningeventwithanincreasedriskformaternalandbaby'sdeath.Theaimofthisstudywastoevaluatetheimpactofpoint-of-careultrasonography(POCUS)onthemanagementandprognosisofthesepatients.MethodsAcaseof31-yr-oldpregnantwomaninourhospital,whowasunderwentPOCUSforevaluatingcardiopulmonaryfunctions,volumestate,fluidresponsivenessandultrasound-guidedprocedureswasadmittedtoIntensiveCareUnit(ICU).Weperformedultrasonographydailyformonitoringorganfunctions.Reviewofrelatedliteratureswasalsoconducted.ResultsWiththehelpofPOCUS,wemadequicklydiagnosisofseverepneumoniaandARDScausedbyH7N9influenza.ThetherapieshadalsobeenchangedafterPOCUSexaminations,suchasrestrictfluidadministrationrelyingontheassessmentsoftheinferiorvenacava(IVC)toestimatepreloadandlungultrasoundmonitoringtoidentifytheearlypresenceofextravascularlungwater(EVLW)andavoidfluidoverresuscitation,ultrasound-guidedrecruitmentmaneuvertoimprovedrespiratorydistresssyndrome,andsoon.ConclusionsPOCUShasasignificantimpactondecision-makingandtherapeuticmanagementandshouldbecomeaclinicalroutineinthemanagementofARDSpatientscausedbyH7N9influenzainpregnancy.