简介:Objective:Todeterminetheroleofhemoglobin(HB)-inducedhemeoxygenase-1(HO-1)ininjuredlungscausedbylimbischemia-reperfusion(I/R)inrats.Methods:Aratmodelofischemiainthehindlimbswasmadebyclampingtheinfrarenalaortawithamicrovascularclip,andlunginjuryoccurredafterreperfusion.ToinducetheexpressionofHO-1inthelungs,Hbwasadministratedintraperitoneallyat16hoursbeforereperfusion.NorthernblottingandWesternblottingwereusedtodetecttheexpressionofHO-1inthelungs,andthecarboxyhemoglobin(COHb)levelinarterialbloodwasassayed.Theeffectofhemoglobin(Hb)ontheinjuredlungsafterlimbI/Rwasdeterminedbymeasuringthechangesoflunghistology,polymorphonuclear(PMN)count,malondialdehyde(MDA)contentandwet-to-dryweightratio(W/D).Zincprotoporphyrin(ZnPP),aninhibitorofHO,wasusedtodeterminewhetherHO-1wasinducedbyHbafterlunginjury.Results:HbledtoasignificantincreaseinHO-1mRNAandproteinexpressioninthelungs,accompaniedbytheincreaseofCOHblevelinarterialblood.Comparedwiththeshamcontrols,thelungPMNcount,MDAcontentandW/Dsignificantlyincreasedat4hoursafterlimbI/R,whichreversedbythepretreatmentwithHbat16hoursbeforereperfusion.ZnPPblockedthisprotectiveroleofHbintheinjuredlungs.Conclusions:HbcaninducethelungHO-1expression,whichplaysanimportantroleinthedefenseagainstI/R-inducedlunginjuryinrats.
简介:Severalaspectsoftheapplicationofsilverelectrodetotheelectrochemicalstudiesofhemoglobinhavebeendiscussedinthispaper.Thesilverelectrodecouldnotonlybeuseddirectlyastheelectrodefortheelectrochemicalstudiesofhemoglobin,butalsoreactwithphenothiazineandbenzimidazoletogivestableandusefulmediator-coatedelectrodes.Inaddition,thesilverelectrodecouldhelpsodiumdodecylsulfatetogivefullplaytoitspromotingeffectontheprotein.
简介:Itiscommonlyseenthatpatientswithbonefractureoftencomplicateotherpartsofinjuriesthatpresentamoreurgentsituationthanfracturesandoftenneedtimelyrecognitionandmanagement.SinceDecember1998,wehavedetectedandanalyzedtheratioofwhitebloodcells(WBC)andhemoglobin(Hb)indiagnosisofcomplicatedinjuriesinpatientswithbonefractureandtrytoraiserescuerateandreducemisseddiagnosisrate.
简介:AmethodconstructinqC~1Piecewisequinticpolynomialoveratriangulargridtointerpo-latefunctionvaluesandpartialderivativesatverticesispresentedinthispaper.Thesetofprecisepoly-nomialsofthismethodisdiscussed.
简介:钴hexacyanoferrate(CoHCF)nanoparticle(尺寸ca。60nm)化学上修改的电极(CME)被制作,在这nanosizedCoHCFCME的血红素(Hb)的电气化学的行为被学习。与一个赤裸的玻璃质的碳电极(GCE)和以一种传统的方式的一般CoHCFCMEelectrodeposited比较,现在的nanosizedCoHCFCME高效地与相对高的敏感,稳定性,和longlife为Hb执行了electrocatalytic减小。与液体层析(LC)结合了,nanosizedCoHCFCME在确定的流动注射被用作Hb的电气化学的察觉者分析电气化学的决心(FIA-ECD)系统。山峰水流是在从2.5楳?景映牯業?捡摩映潲?慣'R湯搠潩楸敤愠摮栠摹潲敧?獵湩?的范围的集中的线性功能?楳楬慣椠浭扯汩穩摥爠瑵敨楮浵挠瑡污獹?獡瀠敲畣獲牯栠獡戠敥?瑳摵敩?湩搠晩敦敲瑮爠慥瑣潩?潣摮瑩潩獮?桔?敲?瑬?敲敶污摥琠慨?桴?佔?琨牵?癯牥映敲畱湥祣?景?佃?愠档敩敶????栠?漠?浩潭楢楬敺?畲桴湥畩?慣慴祬瑳渠慥?桴?牣瑩捩污瀠敲獳牵?潰湩?景??眠瑩??瀠敲獳牵?景???慐?敲捡楴湯琠浥数慲畴敲漠吗????
简介:在低CTAB集中的血红素(Hb)的性质上的cetyltrimethylammonium溴化物(CTAB)的效果被紫外力的光谱,荧光,希腊语的第六个字母潜力,电导率和否定染色的传播电子显微镜(TEM)的方法在Hb/acyclovir/CTAB系统学习。随CTAB集中,在276nm的紫外山峰紧张,内在的荧光,Hb的希腊语的第六个字母潜力和系统的增加,电导率都被提高。Hb容易被氧化到oxyHb和hemichrome。在Hb/acyclovir/CTAB,Hb的系统,成为紫外力的光谱的CTAB,荧光,电导率和符合构造趋于原来的Hb被还给那些但是不那么做的希腊语的第六个字母潜力。Hb-acyclovir建筑群的紫外吸收山峰消失了,并且acyclovir引起的Hb的紧密的结构是refolded。当CTAB集中比5高时;
简介:ThispapershowsthattheC1-curvedfiniteelementdevelopedbyBernadoningeneralcannotsatisfytheessentialboundaryconditionsonapproximateboundary.Furthermore,amodifiedC1-curvedfiniteelementisgiven,whichiscompatiblewiththeelementofArgyristriangleandcansatisfythehomogeneousDirichletboundaryconditionsonapproximateboundary.
简介:A.simplicialmesh(triangulation)isconstructedthatgeneralizesthetwo-dimensional4-directionmeshtoR~m.Thismesh,withsymmetric,shift-invariantvaluesatthevertices,isshowntoadmitaboundedC~1interpolantifandonlyifthealternatingsumofthevaluesattheverticesofany1-cubeiszero.Thisim-pliesthaiinterpolationattheverticesofanm-dimensional,simplicialmeshbyaC~1piecewisepolynomialofdegreem+1withonepiecepersimplexisunstable.
简介:1病例患者,男,63岁,农民,主诉1月前因劳累后,出现两下肢浮肿,无发热、皮疹、咽痛、关节痛及腰痛,在当地医院作"青霉素”及对症治疗无效,且浮肿加重.1周来,发现尿色混浊,呈泡沫状,无排尿痛,经尿常规检查尿蛋白++++,拟诊肾病,收住入院.查体T36.9℃,P84次/min,R20次/min,BP177/74mmHg.神清,呼吸平稳,颜面无明显浮肿,巩膜皮肤未见黄染,未见出血点及瘀点,浅表淋巴结未及,咽不充血,扁桃体不肿大.心律整,未闻及病理性杂音.两肺音清.腹平软,肝脾肋下未及.肾区三联症(-),两下肢中度凹陷性水肿.神经系统未引出病理性反射.化验:血常规WBC5.7×109/L,N0.724,L0.276,Hb9.2g/dl,PLT191×103/μL.尿常规Pro+++,RBC+,24h尿蛋白定量6.62g,尿电泳为非选择性蛋白尿,尿al-微球蛋白22.74mg/L.