简介：Analysisofthefrequencyofantigen-specificcytotoxicTlymphocytes(CTLs)exvivoislargelydependentontheuseofMHC/peptidetetramers.However,thelatterreagentshavenotbeenwidelyavailable,mostlikelybecauseoftheircostlyandtime-consumingproduction.InthisreportweutilizedaneconomicstrategytoconstructHLA/peptidetetramerswithrecombinantpeptide-linkedβ2microglobulin(β2m).TheHLA-A2-restricted,melanomaantigenMARTl-derivedpeptideMARTI27-35(AAGIGILTV)wasfusedtotheNterminusofhumanβ2mthrougha15-aminoacid(aa)-longlinkerbeforebeingrefoldedwiththerecombinantbiotinylatedHLA-A2heavychainectodomain.Theresulted2-component(2C)monomerwasthentetramerizedwithphycoerythin-labeledstreptavidin.Theexperimentalresultshowedthatthe2CHLA-A2/MARTI27-35monomerwasshowntobindtotheHLAclassⅠcomplex-specificmonoclonalantibodyW6/32andtheHLA-A2/MARTI27-35complex-specificsinglechainantibodyfragment(scFv)8.3,suggestingthecorrectnessofitsspecificity.Furthermore,the2CHLA-A2/MARTI27-35tetramerdetectedaspecificCD8^+TcellpopulationinHLA-A2-restrictedmelanomainfiltratinglymphocytesastheconventional3CHLA-A2/MARTI27-35tetramer.Theyieldof2CHLA-A2/MARTI27-35monomerwas2.5timesmorethanthatoftheconventional3Cmonomer.Takentogether,thesedataindicatethattheHLA-A2/MARTI27-35tetramercanbegeneratedconvenientlythroughtheuseofMARTI27-35peptide-β2mfusionproteins,whichcanfacilitatethemonitoringofHLA-A2-restricted,MARTl-specificCTLresponsesinpatientswithmelanoma.

简介：ToselecttheimmunogenicshortpeptidemimicsofmalewormoriginofSchistosomajaponicum(Sj)andtoexploretheirprotectioneffectagainstschistosomiasisinmice,therandomphagedisplaypeptidehbraryof12-merwasscreenedwithIgGtosolublemalewormantigenofSj,andthespecificpositiveclonesselectedthroughthreeroundsofscreeningsweredetectedbyDot-ELISA,andtheninjectedsubcutaneouslyintomiceforvaccinationandprotectionassessmentagainstSj.Itwasfoundthat18randomlypickedphagedisplayedclonesallshoweddefiniteantigenicitywithvariousintensities.Thepooledphagesdisplayedclonescouldinduceproductionofspecificantibodiesandcause31.72%ofwormreductionrateand51.54%ofeggreductionrateinmice,revealingasignificantdifference(P<0.001)incomparisonwiththoseofthecontrols.ItconcludesthattheshortpeptidemimicsofmalewormoriginofSjobtainedbyaffinityscreeningphagedisplayptidelibrarycanelicitpartialprotectionagainstthispathogen.

简介：ThelaserphotolysisofN-diisopropylphosphoryltryptophyltyrosinedipeptidemethylester(N-DiPP-TrpH-TyrOH)hasbeencarriedoututilizingaKrF(248nm)laser.ThedirectintramolecularETbetweenTrpH+residueandTyrOHresiduewasdetectedfirstandtheETbetweenTrpandTyrOHbecameslow.Itwassuggestedthatphosphorylgroupstabilizeneighbourradicalofaromaticringbyhyperconjugation.IncombinationwithcomputermodelingweobtainedthekineticparametersoftheETandprovedthatphosphorylgroupstookpartintheprocessofET.Theresultsshouldbesignificantforbiologicalsystemsinceeverybiologicalprocessinvolvesthephosphorylationornonphosphorylation.

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简介：AIMTo调查vasoactive的角色在形式剥夺的肠的肽(贵宾)近视(频分多路复用).METHODSFDM被放在三组八只小鸡创造一半透明在他们的右眼睛上更弥漫。Intravitreal注射盐并且贵宾一天被使用一次进组的堵塞眼睛2和3分别地。视网膜镜检法和轴的长度(AL)大小在第一和8th天更弥漫穿。在三个组的右眼睛和白天8上的第一个组的左眼睛的VIP受体和ZENK蛋白质的视网膜mRNA层次用在权利的中部的最后的折射(D)看的.RESULTSThe是的实时聚合酶链反应(PCR)被决定-13.75(-16.00,-12.00),-11.50(-12.50,-7.50),和-1.50(在组的-4.75,-0.75)1，2，和3，分别地(P<0.001)。在右眼睛的中部的AL(公里)是10.65(10.00，11.10)，9.90(9.70，10.00)，并且9.20(9.15，9.25)在组1，2，和3，分别地(P<0.001)。为VIP2受体的中部的三角洲三角洲周期阀值(CT)价值是1.07(0.82，1.43)，1.22(0.98，1.65)，0.29(0.22，0.45)在组的右眼睛1，2，和3，和1.18(0.90，1.37)在组1的左眼睛，分别地(P=0.001)。为ZENK蛋白质的中部的三角洲三角洲CT价值是1.07(0.63，5.03)，3.55(2.20，5.55)，在组的右眼睛无法发现1，2，和3和1.89(0.21，4.73)在组1的左眼睛，(P=0.001)分别地，.CONCLUSIONVIP在频分多路复用的发展有潜在的禁止的效果。

简介：Atrialnatriureticpeptide(ANP)isapeptidehormonethathaspotentnatriuretic,diuretic,vasodilator,sympatholytic,andrenin-andaldos-terone-suppressingactivitiesandisinvolvedintheregulationofvolumeandelectrolytebalanceandbloodpressure.Further,ANPhasalsobeenshowntoinhibitcellulargrowth,proliferationandinduceapoptosisinavarietyofcelllines,includingvascularsmoothmusclecellsandcardiacmyocytes.RecentstudieshavedemonstratedthatANPisnotonlyinvolvedinbloodpressureandvolumehomeostasisbutalsointhedirectregulationofcardiacgrowth.Weandotherinvestigatorshavedemonstratedtheexistenceofnatriureticpeptidereceptorsintheheartandcardiaccells,suggestingthatANPhasdirectactionsoncardiactissue.SeveralrecentinvivostudieshavesuggestedthatstatementofANPisinverselyrelatedtocardiacgrowth/hypertrophy.TransgenicmiceoverexpressingANPhavelowerheartweightandbloodpressurethanwildtypemice.Conversely,we

简介：AbstractThe emergence of antimicrobial resistance attributed to the overuse and abuse of antibiotics severely endangers global biosafety. Antimicrobial peptides (AMPs) produced by various living organisms exhibit broad-spectrum antimicrobial properties with a low propensity to the resistance. However, the application of AMPs has been greatly limited owing to their poor stability, high manufacturing cost, and high cytotoxicity. Thus, AMP-mimetic antimicrobial cationic polymers with cationic and amphiphilic moieties have attracted considerable attention as antimicrobial agents. These polymers typically exhibit broad-spectrum antimicrobial activities, negligible antimicrobial resistance, and rapid bactericidal effect. These polymers exhibit low hemolysis and cytotoxicity by optimizing their chemical structures. In this study, we summarize the design principles and current findings of antimicrobial cationic polymers and identify potential candidates for developing innovative polymeric antimicrobials.

简介：Deareditor,Itiswithgreatinterestthatwereadthearticle“Relationshipofcalcitoningene-relatedpeptidewithdiseaseprogressionandprognosisofpatientswithseveretraumaticbraininjury”(Chenetal.,2018).Inthisstudy,theauthorsevaluated121patientswhoweredividedintomild/moderatetraumaticbraininjury(TBI)(n=61),severeTBI(n=35)andcontrol(n=25)groups,andmeasuredserumlevelsofcalcitoningene-relatedpeptide(CGRP)andserumendothelin-1(ET-1).TheyfoundthatlowlevelsofCGRPandhighlevelsofET-1wereassociatedwithhighmortalityat6months.IdentificationofmorphologicalabnormalitiesonCTscansisveryimportantforevaluatingpatientswithTBIbecausedifferentdiagnosesaremadebasedondifferentimagingfindings(Maasetal.,2005).

简介：Identificationofproteinsbymassspectrometry(MS)isanessentialstepinpro-teomicstudiesandistypicallyaccomplishedbyeitherpeptidemassfingerprinting(PMF)oraminoacidsequencingofthepeptide.AlthoughsequenceinformationfromMS/MSanalysiscanbeusedtovalidatePMF-basedproteinidentification,itmaynotbepracticalwhenanalyzingalargenumberofproteinsandwhenhigh-throughputMS/MSinstrumentationisnotreadilyavailable.Atpresent,avastmajorityofproteomicstudiesemployPMF.However,therearehugedisparitiesincriteriausedtoidentifyproteinsusingPMF.Therefore,toreduceincorrectproteinidentificationusingPMF,andalsotoincreaseconfidenceinPMF-basedproteinidentificationwithoutaccompanyingMS/MSanalysis,definitiveguidingprinciplesareessential.Tothisend,weproposeavalue-basedscoringsystemthatprovidesguidanceonevaluatingwhenPMF-basedproteinidentificationcanbedeemedsufficientwithoutaccompanyingaminoacidsequencedatafromMS/MSanalysis.

简介：抗菌剂肽是有抗菌剂活动的多肽。从中国虾(FenneropenaeusChinensis)的抗菌剂肽基因Np3和Np5集成于OryzasativaL。subsp。装饰用的梨树cv。由Agrobacterium的Aichiashahi调停了转变系统。PCR分析证明Np3和Np5的积极比率分别地在T0产生是36%和45%。RT-PCR分析证明抗菌剂肽基因在T1产生被表示，并且在转基因的植物和非转基因的植物之间的农学的特点地没有明显的差别。四Np3和Np5在T1产生的转基因的线与Xanthomonasoryzaepv被接种。oryzae紧张CR4，和所有四根转基因的线显著地提高了抵抗到紧张CR4引起的细菌的老家。Np5转基因的线也显示出更高的抵抗到紧张JS97-2，Zhe173和OS-225引起的细菌的老家。有Np5基因力量的转基因的线拥有宽广光谱抵抗到米饭，这被建议细菌的老家。

简介：TofindouttheprotectivepolypeptideepitopesofHCVHVR1,theantigenieityofthesyntheticpepfidewaspredictedbycomputermodelingandverifiedbyELISAandlymphocytetransformationtest.Itwasfoundthattheoutcomeofthecomputermodelingwasinaccordwiththeexperimentalresults.Themethodbyusingcomputermodelingwouldbeaeconomicapproachbywhichtheprotectivepeptidescouldbeidentifiedquickly.

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简介：Thelabellingandimagingoftumorcellswereinvestigatedviaarginine-glycine-asparticacidcysteine(RGDC)peptide-labelledquantumdots(QDs).TheresultsshowthatRGDCmodifiedQDscanlabelSMMC-7721tumorcellsandadheretocellularmembrane.Inconstrast,theunmodifiedQDsaremainlydispersedaroundthecell.WealsofoundthattheRGDC-QDscanpenetrateintothecellat2hofincubation.After6hofincubation,RGDC-QDscanaccumulateinauniqueintracellularregion.

简介：Peptidenucleicacids(PNAs)aresyntheticoligonucleotideswithchemicallymodifiedbackbones.PNAscanbindtobothDNAandRNAtargetsinasequence-specificmannertoformPNA/DNAandPNA/RNAduplexstructures.Whenboundtodouble-strandedDNA(dsDNA)targets,thePNAmoleculereplacesoneDNAstrandintheduplexbystrandinvasiontoformaPNA/DNA/PNA[or(PNA)2/DNA]triplexstructureandthedisplacedDNAstrandexistsasasinglestrandedD-loop.PNAhasbeenusedinmanystudiesasresearchtoolsforgeneregulationandgenetargeting.TheDloopsgeneratedfromthePNAbindinghavealsobeendemonstratedforitspotentialininitiatingtranscriptionandinducinggeneexpression.PNAprovidesapowerfultooltostudythemechanismoftranscriptionandaninnovativestrategytoregulatetargetgeneexpression.AnunderstandingofthePNA-mediatedgeneregulationwillhaveimportantclinicalimplicationsintreatmentofmanyhumandiseasesincludinggenetic,cancerous,andage-relateddiseases.

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简介：在大数字在家蝇(蝇属domestica)发现的抗菌剂和抗真菌的肽是它的有免疫力的防卫机制的不可缺少的部件。在这研究，家蝇的幼虫的前面的尖端与一双好剪刀被割掉，hemolymph在镇定、渗出一冰冷试管。从hemolymph，一种抗真菌的物质被与反向的阶段高度表演液体chromotography(RP-HPLC)相结合并且作为蝇属domestica说出抗真菌的peptide-1(MAF-1)的稳固阶段的抽取孤立。钠dodecylsulphate-polyacrylamide胶化电气泳动(SDS页)证明它的分子的重量是17kDa。紫外吸收系列表明这种抗真菌的物质拥有了蛋白质肽的特征。由指纹鉴定和双人脚踏车团spectrometry的分析建议MAF-1是未知蛋白质。Edman降级识别了它没在吉祥物搜索数据库匹配肽的N终端的30氨基酸的顺序，显示MAF-1是一只新奇昆虫抗真菌的肽。集体spectrometry证明MAF-1的精确分子的重量是17203.384Da。它的等电位的点是酸的。

简介：CMV特定的免疫为人的cytomegalovirus(HCMV)的控制是必要的感染。干细胞移植在hemopoietic系统的疾病的一个范围的管理广泛地被使用。病人们在早posttransplant时期深刻地是immunosuppressed，并且cytomegalovirus(CMV)的复活仍然是病态和死亡的一个重要原因。CMV特定的CD8+T房间克隆的采纳转移被显示了减少病毒的复活的率；然而，为采纳转移的房间的前vivo生产是集中、昂贵的劳动。我们这里用CMV特定的epitope肽报导一个修改肽刺激方法为CMV特定的CTL的产生刺激PBMC。这个方法允许CMV特定的CTL的有效扩大并且从一件单个血样品为FACS分析提供很多房间。显著地，它完成允许容易要比较并且顺序评估了的不同个人的数据的CD8+T房间染色的tetramer的高频率。因此，这条途径为潜在的采纳免疫疗法扩展并且选择高特性的限制HLA的CMV-pp65-reactiveT房间线。

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简介：无机的多面的oligomericsilsesquioxane(POSS)为合成被用作核心poly(l离氨酸)经由催化铜的azidealkyne按化学的肽dendrimer。无机/器官的合成dendrimer被MS，1HNMR，FTIR，GPC和DLS描绘。

简介：C类型natriuretic肽(CNP)是调停的本地规章的因素的一种最新发现的类型它通过specic的生物效果，膜界限natriuretic肽receptor-B(NPR-B)。最近的研究证实了CNP是仔细与男繁殖功能有关。这研究的目的是通过不同方法在人的呼喊的精子决定CNP/NPR-B的分发，然后在精子功能invitro上评估CNP的影响(例如immunolocalization，实时聚合酶链反应和西方的污点)，例如活动性和acrosome反应。人的精液样品从同意的施主被收集为normozoospermia满足了世界健康组织的标准。我们的结果证明CNP的specic受体NPR-B在头和精子的前端尾巴的膜的acrosomal区域是局部性的，并且没有在人的精子的CNP的信号。与控制相比，CNP能在精子活动性和acrosome反应导致signicant剂量依赖者增加。在摘要，CNP/NPR-B能影响精子活动性和acrosome反应，因此调整男性的繁殖功能。CNP可以是在调整精子功能的一个新关键因素。