简介：OPM05-M2100,thespecificRAPDfragmentofBursaphelenchusxylophilus,wascollectedfromagarosegelsandpurified.Then,thepurifiedfragmentwasinsertedintothepGEMR-TVectorthatwastransformedintoEscherichia.coliandclonedandsequenced.BasedonthesequenceofRAPDmarker,thesequencescharacterizedamplifiedregion（SCAR）primersweredesignedbytheaidofthesoftwareOligo5.0.TheforwardprimerisM05F2（5’-CGGGTCATGGCTGGAGGTATCGT-3’）,andthebackwardprimerisM05R1(5’-TGGCTCAATGGCAAATCCTTCGTA-3’.Thespecificfragment(OPM05-M2100)wassuccessfullyconvertedtoSCARmarker(SCAR-M05-X600)byusingM05F2/R1,whichwasthespecificmarkersofB.xylophilus.Then,theDNAof92isolatesofBursaphelenchus,B.mucronatus,B.hofmanni,AphelenchoidesmacronucleatusandSeinurasp.whichwereisolatedfromdeadpines,weremarked,andtheDNAofasinglenematodeextractedwithasimplemethodwasdetectedusingthissetofspecificprimers.TheresultsindicatedthatthePCRproductofall81isolatesofB.xylophiluswasaclearandbrightfragmentabout600bpwithM05F2/R1.ButeightisolatesofB.mucronatus,oneB.hofmanni,oneA.macronucleatusandoneSeinurasp.hadnoanyfragments.AssayM05F2/R1alsosuccessfullydetectedsinglepinewoodnematode.Therefore,thespecificpairwiseswouldbeusedforconstructingidentificationkitsofB.xylophilus,implementingtheaimofquickdetection,andachievingthepurposeofidentifyjuvenilesuccessfully.

简介：Molecularmarkershavebeenwidelyusedincropgeneticimprovement,seedtestandgeneticmapping.Ofwhich,sequencecharacterizedamplifiedregion(SCAR)markersareparticularlypopularforitsdiversity,stablereproducibility,andsuitabilityforanalyzinglargenumberofsamples.Inthisstudy,500randomamplifiedpolymorphicDNA(RAPD)primersweretested,andasetofSCARmarkerscomprising37pairsofloci-specificprimersweredevelopedfromtheDNAfragmentsrangingfrom300to1000bpwhichcorrespondtothestable,distinctiveRAPDbandingpatterns.UsingtheseSCARmarkers,59hybridricecombinationswereassessedanddistinguishedinto58subgroupsatthesimilaritycoefficientof0.97inageneticclusteringtreebasedontheallelediversitiesoftheSCARmarkers.Furthermore,13hybridricecombinationswerereassayedwith40randomlyselectedsimplesequencerepeat(SSR)markerstoevaluatetheeffectivenessoftheseSCARmarkers.SSRmarkersproducedsimilarresultstoSCARmarkersasthe13hybridricecombinationswerecompletelyseparatedatthesimilaritycoefficientof0.91intheclusteringtreeestablishedfromSSRpatterns.Takentogether,SCARmarkersprovetobeeffectivetoolsforidentifyinganddifferentiatinghybridricecombinations.

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简介：Thecalciumchannelblocker,verapamil,hasbeenshowntoreducescarformationbyinhibitingfibroblastadhesionandproliferationinvitro.Itwasnotclearwhethertopicalapplicationofverapamilaftersurgicalrepairofthenerveinvivocouldinhibittheformationofexcessivescartissue.Inthisstudy,therightsciaticnerveofadultSprague-DawleyratswastransectedandsuturedwithNo.10-0suture.Thestomawaswrappedwithgelfoamsoakedwithverapamilsolutionfor4weeks.Comparedwiththecontrolgroup(stomawrappedwithgelfoamsoakedwithphysiologicalsaline),theverapamilapplicationinhibitedthesecretionofextracellularmatrixfromfibroblastsinvivo,suppressedtypeIandIIIcollagensecretionandincreasedthetotalnumberofaxonsandthenumberofmyelinatedaxons.Thesefindingssuggestthatverapamilcouldreducetheformationofscartissueandpromoteaxongrowthafterperipheralnerverepair.

简介：应美国缅因州大学气候变化研究所P．Mayewski教授的邀请并经中国气象局国际合作司批准，国家气候中心阎宇平博士于2004年7月25～30日参加了在德国布莱梅举行的“第28届国际南极研究科学委员会(SCAR)大会——全球系统中的南极洲和南大洋”，

简介：霜霉病是由Peronosporatabacina引起的一种叶部病害，在生产中是一个严重的问题。在田间霜霉病的发生受环境的影响很大，使得抗病育种的过程更加复杂。

简介：X-irradiationhasabeneficialeffectintreatingspinalcordinjury.WesupposedthatX-irradiationcouldimprovethemicroenvironmentatthesiteofaspinalcordinjuryandinhibitglialscarformation.Thus,thisstudywasdesignedtoobservetheeffectsof8GyX-irradiationontheinjurysiteat6hoursand2,4,7,and14dayspostinjury,intermsofimprovementinthemicroenvironmentandhindlimbmotorfunction.ImmunohistochemistryshowedthattheexpressionofmacrophagemarkerED-1andtheareawithglialscarformationwerereduced.Inaddition,theBasso,BeattieandBresnahanscorewashigherat7dayspostinjuryrelativetotheothertimepointspostinjury.ResultsindicatedthatX-irradiationatadoseof8Gycaninhibitglialscarformationandalleviatetheinflammatoryreaction,therebyrepairingspinalcordinjury.X-irradiationat7dayspostspinalcordinjurymaybethebesttimewindow.

简介：以东北林业大学帽儿山实验林场天然白桦为研究群体,通过分析其中有代表性的100株个体的纤维长度与RAPD分子标记间相互关联性,筛选出一条与长纤维长度相关性系统为0.6144、显著性达1%水平的片段"BFLR-16",对此片段进行克隆、测序后,并将其转化成与长纤维性状相关的SCAR标记。此标记对长纤维白桦的鉴定效率高达92%,说明此SCAR标记对长纤维个体具有高度特异性,与控制纤维性状长度的基因高度连锁,它的存在与白桦纤维长度的增加有明显的相关性。图6参16。

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简介：Objective:Todeterminetheefficacyofpolylacticacidglueinpreventingepiduralscaradhesionafterlaminectomyinrabbits.Methods:Twenty-fourJapanesewhiterabbitsunderwentlaminectomy(includingtheattachedligaments)atL2andL5.AfterlaminectomyatL5,polylacticacidgluewassprayedontheduraandnerverootsandthissegmentwastakenastheexperimentalgroup.AfterlaminectomyatL2,nothingwasusedandthissegmentwasenrolledastheselfcontrolgroup.Fourrabbitswerekilledeverytwoweekspostoperativelytilltheendoftheexperimentat12weeks.Thentheoperatedspinewasobservedgrossly,histologicallyandultrastructurallytocheckthedegreeofscarformation,thestatusofepiduralscaradhesion,theabsorptionoftheglue,andtheintracellularstructureoffibroblasts.Results:Thegluecoagulatedimmediatelyaftersprayingandshowedexcellenthemostaticeffect.Thegluemembranewaseasytobetakenawayfromtheduramaterofthesamplesfor2weeksandtherewerenocellsintheepiduralspaceintheexperimentalgroup.Buttheduramaterwascoveredbyhematomainthecontrolgroup,whichformedmildadhesion,withfibroblastsproliferatingactively.Inthe4thweek,someglueshiversremainedintheepiduralspacewithfibroblastsincreasingalittle,andtheduramaterwassmoothintheexperimentalgroup.However,inthecontrolgroup,theformedscarwasfragileandconglutinatedwiththeduramaterdiffuselyandfibroblastsweremuchmorethanthoseintheexperimentalgroup.Inthe6th-12thweeks,therewasapotentialinterspacebetweenthescarandtheduramater,andthepolylacticacidgluewasabsorbedcompletelyintheexperimentalgroup.Muchtoughscarwasfoundinthecontrolgroup,whichwasverydifficulttodissectfromtheduramaterandthesurroundingtissues.Fromtheultrastructuralobservationofthefibroblasts,thenucleusbecamemuchbiggerandtheroughendoplasmicreticulumwasmuchmoreplentifulinthecontrolgroupthanthatinthee

简介：Substratecharacterizationisthemainstayofablationforventriculartachycardia(VT).Althoughtheuseofelectroanatomicvoltagemapping(EAVM)intheelectrophysiology(EP)laboratoryhasenabledreal-timeapproximationofmyocardialscar,ithaslimitations.Thisisrelatedtothesubjectiveandtediousnatureofvoltagemappingandthechallengesofdefiningthetransmuralityofscar.Variousnoninvasivemethodsofscarassessmenthaveemerged,withmagneticresonanceimaging(MRI)beingthemostaccurate.IntegratedMRIandelectroanatomicvoltagemappingstudiesdemonstrategoodcorrelation.Nonetheless,MRIhasadvantages.Theseinclude(1)preprocedureidentificationofepicardialandintramuralscar,(2)assessmentofablativelesionformationafterunsuccessfulablations,(3)identifi-cationofheterogeneousregionsofscar,wherecriticalconductingchannelsarelikelytooccur,and(4)predictivevalueintheassessmentofsuddencardiacdeath(SCD).Integrationofscarimaginginventriculartachycardiaablationandriskstratificationhasgreatpotentialtoadvancethepracticeofarrhythmiamanagement.

简介：Centralnervoussystem(CNS)injuriescausedbycerebrovascularpathologies(e.g.,stroke)ormechanicalcontusions(e.g.,traumaticbraininjury)disrupttheblood-brainbarrier(BBB)thatprotectstheCNSmicroenvironmentfromadirectcontactwithbloodsubstancesandcells.Theinitialneuraldamagecausedbythetraumaandtheischemicprocess

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简介：Amathematicalmodelhasbeenformulatedinaccordancewithcellchemotaxisandrelevantexperimentaldata.Athree-dimensionallatticeBoltzmannmethodwasusedfornumericalsimulation.Thepresentstudyobservedtheeffectsofglialscarsizeandinhibitorconcentrationonregenerativeaxonalgrowthfollowingspinalcordtransection.Thesimulationtestcomprisedtwoparts:(1)whenreleaseratesofgrowthinhibitorandpromoterwereconstant,theeffectsofglialscarsizeonaxonalgrowthratewereanalyzed,andconcentrationsofinhibitorandpromoterslocatedatthemovinggrowthconeswererecorded.(2)Whentheglialscarsizewasconstant,theeffectsofinhibitorandpromoterreleaseratesonaxonalgrowthratewereanalyzed,andinhibitorandpromoterconcentrationsatthemovinggrowthconeswererecorded.Resultsdemonstratedthat(1)alargerglialscarandahigherreleaserateofinhibitorresultedinareducedaxonalgrowthrate.(2)Theaxonalgrowthratedependedontheratioofinhibitortopromoterconcentrationsatthegrowthcones.Whentheaverageratiowas<1.5,regeneratingaxonswereabletogrowandsuccessfullycontacttargetcells.

简介：利用SCAR引物862对288份黄瓜自交系进行黑斑病抗性检测，结果有52份资源抗病。通过对56份资源与人工接种抗性鉴定结果相比较，二者的符合率达94．44％；对76份田间表现感病的材料进行标记检测，4份材料具有抗病带，符合率达94．74％。在育种应用中，可先通过标记初步进行大量材料的抗性检测，在此基础上根据需要有针对性地做人工接种抗病性鉴定，该方法可以有效提高黄瓜种质资源抗性筛选的效率。抗黑斑病资源的获得，为进行抗黑斑病黄瓜新品种的选育奠定基础。

简介：应用序列特异性扩增区域（SCAR）标记技术分析50株黑木耳栽培菌株的遗传多样性。在SRAP标记分析过程中发现1条1200bp的特异性条带,经回收克隆测序转为SCAR标记。根据序列设计出1对特异性引物,经PCR可以扩增出1000bp大小的片段,说明成功构建出了＂黑931＂的指纹图谱。

简介：AbstractObjective:To compare the efficacy and safety of four surgical strategies currently used for the management of deep implantation cesarean scar pregnancy (CSP-II).Methods:This was a retrospective clinical cohort study, and, in total, 131 women diagnosed with CSP-II and primarily treated in our hospital were recruited. Women treated using laparoscopy assisted by operative hysteroscopy (LAOH; Group A, n = 25), uterine artery embolization (UAE) followed by LAOH (Group B, n = 21), ultrasound-guided dilatation and curettage (D&C; Group C, n = 24), and UAE followed by D&C (Group D, n = 61) were evaluated. Univariate and multiple logistic analyses were performed to identify the risk factors.Results:No statistically significant difference was found in patient age, gestational age, size of lesion, and pretreatment serum β-human chorionic gonadotropins (β-hCG) level. Operation time was longer (P < 0.001) and the success rate was higher (P = 0.01) in both Group A and Group B than in Group C and Group D. When the cohort was further analyzed regarding patients with myometrial thickness ≤3 mm (n = 75, defined as CSP-IIb), a lower rate of perioperative complications (P = 0.036) and a higher success rate (P < 0.001) remained in Group A (n = 15) and Group B (n = 15) but not in Group C (n = 11) or Group D (n = 34). In multiple logistic regression analysis, the risk factors related to lower treatment efficacy for patients with CSP-II were thinner myometrial thickness of cesarean scar (CS) (≤3 mm) (odds ratio [OR] = 5.470, P = 0.062), number of cesarean sections (a2) (OR = 8.877, P = 0.013), mass protruding into the bladder or abdominal cavity (OR = 25.507, P < 0.001), and direct D&C modality (OR = 38.247, P = 0.010).Conclusions:Compared with D&C ± UAE, LAOH ± UAE showed a higher success rate for patients with CSP-II, especially when the zygote was more deeply implanted with a myometrial thickness of CS ≤ 3 mm. CSP-II treatment should be individualized on the basis of many risk factors.

简介：在对60个供试黑木耳菌株RAPD指纹图谱进行分析的基础上获得了黑木耳菌株Au185的RAPD特异性标记，将其克隆、测序，应用Primer5．0软件设计相应的特异引物对SC38—1，将RAPD特异性标记转化为黑木耳菌株Au185的稳定方便的SCAR标记，可快速、准确地鉴定出黑木耳菌株Au185。

简介：客观：在单个教材扩大(矿泉)和高密度的oligonucleotideDNA数组的帮助下学习在earliergestational皮肤和老鼠的以后的gestational皮肤之间的基因表示的差别理解scarlesshealing的分子的机制。方法：全部的RNA从疤更少(E15)的胎儿的老鼠皮肤被孤立怀孕期(学期=21.5天)的形成andscar(E18)时期。从更早的gestational皮肤(EGS)和以后的gestational皮肤(LGS)的RNA相对地两个都被抄录到cDNAs，然后为由矿泉方法准备杂交探针用荧光灯的dCTP的加入标记。Themixed探针当时是到包含了5的oligonucleotideDNA数组的hybridized代表5705老鼠基因的705根探针。在高度紧的洗以后，这些DNA数组被扫描因为显示差别的荧光灯的信号表示了在2组skin.Results之间的基因：在5705老鼠基因之中，与差别有53基因(0.93%)在EGS和LGS组，之间的表示层次27基因，包括成纤维细胞生长因素2(FGF2)并且follistatinwere起来调整(0.47%)并且26基因是下面调整的(0.46%)在胎儿的皮肤在scarlessperiod对形成疤的时期期间。在EGS的FGF2和follistatin的更高的表情被RT-PCR方法也比那些inLGS揭示。结论：高密度的oligonucleotideDNA数组为调查微分基因表示在提供了一个强大的工具更早并且latergestational胎儿的皮肤。这种技术验证胎儿的疤更少愈合的机制是很复杂化，许多基因表情的变化与胎儿的疤被联系更少的愈合。

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