简介：Selenium(Se)isanessentialtraceelement,whichhumansandanimalstakedirectlyorindirectlyfromplants.Thispaperreviewedtherecentresearchprogressonspeciationofseleniumcompoundsinplants,includingthekindsofseleniumcompoundsandtheirmetabolicpathways,andthetechnologyforsampletreatment,separation,anddetermination.Organicseleniumcompoundsarethemajorpartsoftheseleniumspeciationinplants.Thetechnologyofultrasonic-microwavesynergisticextractioncombinedwithenzymatichydrolysisisusefulinsamplepreparation.HPLC-ICP-MSiswidelyusedindeterminingseleniumspeciation.Itisimportanttodevelopmorestandardseleniumcompoundsandpaymoreattentiontoselenoproteininplants.ESMS,ESMS-MSandMALDI-TOFcanbeappliedtoidentifyingtheunknownseleniumcompounds.TheapplicationofIDAindeterminingboththemetabolicpathwaysandthestructureoflargeseleniummoleculeswillbeamajoraspectforthefutureresearch.

简介：Amicrofluidicsystemwasdevelopedforthesynthesisoftrigonalselenium(t-Se)nanowires,whichwascomposedofaglassmicrochipcoupledwithapoly(methylmethacrylate)(PMMA)microchip.Intheglassmicrochip,amorphousselenium(a-Se)colloidwaspreparedbyreducingseleniousacidwithanexcessamountofhydrazineatatemperatureof100℃.InthecoupledPMMAmicrochip,a-Sewastransformedintomorestablet-Seseedsviasonicationatroomtemperature.Theresidencetimeofthereactantsinbothmicrochipswasoptimizedbyvaryingthedimensionandlengthofthemicrochanneleach.Thet-Senanowireswereformedbyanisotropicgrowthofseleniumcrystalliteduringsonicationandagingundertheassistanceofβ-cyclodextrin(β-CD).Variousstagesofthenanowires'growthwereinvestigated.Theas-synthesizedproductswerecharacterizedbypowderX-Raydiffraction(XRD),Ramanspectroscopy,scanningelectronmicroscopy(SEM),transmissionelectronmicroscopy(TEM)andselected-areaelectrondiffraction(SAED).

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简介：离子的液体的一种新类型支持了硒试剂被综合并且发现了面对30%H2O2是在到醛和酉同类的白酒的氧化的优秀催化剂。离子的液体的可预言的溶解度从反应混合允许氧化产品的容易的分离。而且，当溶剂，和离子的支持液体的硒试剂能被再循环并且在催化性能与小减少在四次使用了，氧化反应能用离子的液体被执行。

简介：摘要：随着人们对软件质量的重视，使得软件测试技术不断受到人们的关注。相比较于传统的人工测试，自动化测试能够大大提高测试效率。Selenium一个用于Web应用程序测试的工具，有一套完整的Web应用程序测试系统，使用Selenium与Python技术结合，能方便得实现自动化测试。

简介：在从贵州省的土样的硒的内容，它盖住17.6x10~的一个区域4km~2被决定了。947件样品的算术平均价值是0.39mg/kg，中等价值是0.30mg/kg，硒的内容在在基岩上开发的土壤从0.06～1.29mg/kg变化，它被基岩自己控制。

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简介：Extendedx-ray-absorptionfine-structuremeasurements(EXAFS)wereperformedonnanocrystalline(nc)elementalSesampleswithgrainsizesrangingfrom13to60nm.Accompaniedwiththepreviousstudy,weconcludedthat,withtherefinementinnc.Se.theintrachainstructure(thebondlength,thecoordinationnumber)isunchangedwhiletheinterchainspacingisenlarged.ThegrainboundaryinthencSeisfoundtobeinalow-energyconfigurationthatisdifferentfromthedisorderedgaslikegrainboundarystructure.

简介：摘要本文通过对当前典型的自动化测试框架的研究，在开源工具的基础上，设计了分层WEB自动化测试框架，实现了界面元素、测试数据的统一管理，支持数据驱动和测试流程自定义控制。

简介：ＩＮＦＬＵＥＮＣＥＯＦＺＩＮＣ，ＭＡＮＧＡＮＥＳＥＡＮＤＳＥＬＥＮＩＵＭＯＮＳＵＰＥＲＯＸＩＤＥＤＩＳＭＵＴＡＳＥＡＣＴＩＶＩＴＹＩＮＬＵＮＧＣＡＮＣＥＲＴＩＳＳＵＥＡＮＤＣＥＬＬＩＮＣＵＬＴＵＲＥＹｕＬｕｎｙｉｎ喻伦银；ＸｉａＤｏｎｇ夏东；（Ｄｅｐ...

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简介：温室实验被进行在在正常、含硒的土壤上种的二个米饭变化(PR116和PusaBasmati1121)调查硒累积和它的抗氧化剂反应。植物生长在早发展的阶段被减少，flowering被10d的一个时期在含硒的土壤上推迟。3–20和13–14增加的硒累积在叶子合拢，在从PusaBasmati1121和PR116变化的谷物的18和3褶层分别地。在从在含硒的土壤上种的大米植物的叶子的硒累积导致了叶绿素内容，氢过氧化物，脯氨酸，免费氨基酸，全部的酚和丹宁内容的重要增加。而过氧化氢酶活动显示出一个反向的趋势，在叶子的类脂化合物peroxidation层次和peroxidase活动增加了。硒累积减少了，这被结束在在庄稼期间，开发而是这些种的米饭的干燥的事内容能由在他们的防卫系统导致改变与硒毒性作斗争。

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简介：Toinvestigatethetreatmenteffectof2-seleniumbridgedβ-cyclodextrin(2-SeCD),aGPXmimic,onthestrokeofstroke-pronespontaneouslyhypertensiverats(SHRSP),fifty-twoSHRSPof8-weekoldwererandomlydividedintofourgroupsA,B,CandcontrolgroupD.TheratsofgroupsA,B,CandDweregiven1.0%-1.5%NaClmassfractionasdrinkingfluid.Afteronsetofstroke,groupsA,BandCweregivenorally16.05,160.5and1605mg*kg-1*day-1of2-SeCD,respectively,andgroupDwasgivenwaterfor2weeks.Theclinicalscoreofstroke,systolicbloodpressure(SBP),survivaltimeofratswererecordedandthehistopathologicexaminationsoftheirbrainandcarotidarteryweremadeafterdecapitation.Theclinicalscoresofstrokeaftertreatmentwith160.5mg*kg-1*day-1(GroupB)and1605mg*kg-1*day-1(GroupC)of2-SeCDare2.55±0.98and1.98±0.79,respectively,thoseareobviouslylowerthanthatofgroupD(3.41±0.83,p<0.01).ThesurvivaldaysingroupB(10.0±8.6)andgroupC(14.4±7.9)arelongerthanthatforgroupD(4.7±2.9,p<0.01).TheelectronmicroscopestudyshowedthattheendotheliumofcarotidarterywasneartonormalingroupBandgroupC,whileitwasseriouslyinjuredincontrolgroupDandmildlyinjuredingroupA.2-SeCDmayeffectivelybeusedtotreatthestrokeforSHRSP.

简介：过氧化氢酶(猫)和硒依赖者谷胱甘肽peroxidase(Se-GPx)在由消除H2O2。这篇论文的目的是响应低咸度应力在ridgetail白人对虾Exopalaemoncarinicauda评估这些抗氧化剂分子的角色。包含猫的完全的编码顺序的互补DNA(cDNA)用反向抄写的聚合酶链反应(RT-PCR)和cDNA结束的快速的扩大从hepatopancreas被克隆。猫(2649bp)的全身的cDNA包含78bp的一个5-untranslated区域(UTR)，1017bp的3-UTR，与一poly(A)尾巴，并且开的读与58.46kDa的预言的分子的质量和6.64的估计的等电位的点编码517-amino-acid多肽的1554bp的框架。这个猫序列包含了近似活跃地点签名(60FDRERIPERVVHAKGAG76),近似heme-ligand签名顺序(350RLFSYPDTH358)和三催化氨基酸残余(他的71,Asn144和Tyr354)。顺序比较证明CAT推出了E的氨基酸顺序。carinicauda另外的种类与那些分享了身份的68%-92%。量的即时PCR分析表明猫mRNA广泛地在hepatopancreas被表示(最高)，血球，眼柄，心，鳃，肌肉，卵巢和胃。在低咸度应力下面，猫和GPxmRNA表示在分别地在开始的48h和6h显著地增加的鳃和hepatopancreas铺平两个，显示在E的织物依赖者和时间依赖者抗氧化剂回答。carinicauda。所有这些结果显示那E。carinicauda猫是CAT家庭的一个成员并且可能对低咸度应力涉及尖锐反应。

简介：Inordertoenhancetheglutathioneperoxidase(GPX)catalyticactivityoftheselenium-containingsingle-chainvariablefragments(Se-scFv),anovelhumanscFvwasdesignedonthebasisofthestructureofhumanantibodyandoptimizedviabioinformaticsmethodssuchashomologoussequenceanalysis,three-dimensional(3D)modelbuilding,binding-siteanalysisanddocking.TheDNAsequenceofthenewhumanscFvwassynthesizedandclonedintotheexpressionvectorpET22b(+),thenthescFvproteinwasexpressedinsolubleforminEscherichiacoliBL21(DE3)andpurifiedbyNi2+-immobilizedmetalaffinitychromatography(IMAC).TheserineresidueofscFvintheactivesitewasconvertedintoselenocysteine(Sec)withthechemicalmodificationmethod,thus,thehumanSe-scFvwithGPXactivitywasobtained.TheGPXactivityoftheSe-scFvproteinwascharacterized.ComparedwithotherSe-scFv,thenewhumanSe-scFvshowedsimilarefficiencyforcatalyzingthereductionofhydrogenperoxidebyglutathione.ItexhibitedpHandtemperaturedependentcatalyticactivityandatypicalping-pongkineticmechanism.