简介：日前．《现代物料搬运》杂志发布了对制造仓储和配送行业物料搬运现状的调查结果。调查显示给这三个行业的经理人带来压力的13个备选项中，成本是最大的挑战，分列二到五位的是公司成长、吞吐量职业安全和培训。事实上按提及率排序前五项十分接近，89％的人选择了成本牵制，培训也有61％的提及率。

简介：每当进入年末，影音媒体的器材评选结果会不断地推出。尽管此类结果会以“榜单”、“最好的XX”等名字出现，但表述的都是被某一媒体所认可的影音器材的评选结果。早年发烧友对这种“榜单”是很敬重的，就连经销商也会以此作为选择品牌及进货的参考，但随着传统影音市场的变革及不景气，媒体的日子也不像昔日那么好过，加上一些新冒出的媒体的不负责任的器评及评选（有些品牌根本是听也没听过、见也未见过却赫然上榜，此种事例已不在少数），

简介：Inordertounderstandthedevelopmentofstemcellsintospecializedmaturecellsitisnecessarytostudythegrowthofcellsinculture.Forthispurposeitisveryusefultohaveanefficientcomputerizedcelltrackingsystem.Inthispaperaprototypesystemfortrackingneuralstemcellsinasequenceofimagesisdescribed.Inordertogetreliabletrackingresultsitisimportanttohavegoodandrobustsegmentationofthecells.Toachievethiswehaveimplementedthreelevelsofsegmentation.Theprimarylevel,appliedtoallframes,isbasedonfuzzythresholdandwatershedsegmentationofafuzzygrayweighteddistancetransformedimage.Thesecondlevel,appliedtodifficultframeswherethefirstalgorithmseemstohavefailed,isbasedonafastgeometricactivecontourmodelbasedonthelevelsetalgorithm.Finally,theautomaticsegmentationresultonthecrucialfirstframecanbeinteractivelyinspectedandcorrected.Visualinspectionandcorrectioncanalsobeappliedtootherframesbutthisisgenerallynotneeded.Forthetrackingallcellsareclassifiedintoinactive,active,dividingandclusteredcells.Differentalgorithmsareusedtodealwiththedifferentcellcategories.Aspecialbacktrackingstepisusedtoautomaticallycorrectforsomecommonerrorsthatappearintheinitialforwardtrackingprocess.

简介：我们检验了在一个xeno移植动物的细胞建模的人的胚胎的茎(hES)的myogenic潜力。这里，我们证明区分开来与hES房间的先锋能在成年环境经历myogenesis并且在myogenic系产生房间类型的一个范围。这研究提供hES房间能在vivo改革肌肉和卫星房间并且是为对待肌肉的另一种有希望的房间类型的直接证据除了另外的myogenic房间类型的退化混乱。房间研究(2006)16：713-722。做i：10.1038/sj.cr.7310080；出版联机2006年6月20日。

简介：Theconceptsofstemcellsbeingresistanttotherapy,stem-likecellsexistinginbraintumors,andthesetumorsinitiallyrespondingto

简介：Mammaliancelltotipotencyisasubjectthathasfascinatedscientistsforgenerations.AlonglastingquestionwhethersomeofthesomaticcellsretainstotipotencywasansweredbythecloningofDollyattheendofthe20thcentury.Thedawnofthe218thasbroughtforwardgreatexpectationsinharnessingthepoweroftotipotentcyinmedicine.Throughstemcellbiology,itispossibletogenerateanypartsofthehumanbodybystemcellengineering.Considerableresourceswillbedevotedtoharnesstheuntappedpotentialsofstemcellsintheforeseeablefuturewhichmaytransformmedicineasweknowtoday.Atthemolecularlevel,totipotencyhasbeenlinkedtoasingulartranscriptionfactoranditsexpressionappearstodefinewhetheracellshouldbetotipotent.NamedOct4,itcanactivateorrepresstheexpressionofvariousgenes.Curiously,verylittleisknownaboutOct4beyonditsabilitytoregulategeneexpression.ThemechanismbywhichOct4specifiestotipotencyremainsentirelyunresolved.Inthisreview,wesummarizerethestructureandfunctionofOct4andaddresstoOct4functioninmaintainingtotipotencyorpluripotencyofembryonicstemcels.

简介：Objective:Toexplorethecultureconditionsofhumanneuralstemcellsandtoinvestigatetheultrastructureofneurospheres.Methods:Thecellsfromtheembryonichumancorticesweremechanicallydissociated.N2mediumwasadaptedtocultureandexpandthecells.ThecellswereidentifiedbyimmunocytochemistryandEMwasappliedtoexaminetheultrastructureofneurospheres.Results:Theneuralstemcellsfromhumanembryonicbrainsweresuccessfullyculturedandformedtypicalneurospheresinsuspension,andmostofthecellsexpressedvimentin,whichwasamarkerforneuralprogenitorcells,andthecellscoulddifferentiateintoneurons,astrocytesandoligodendrocytes.Invitromyelinformationinneurosphereswereobservedatanearlystageofculture.Conclusions:Humanneuralstemcellscanbeculturedfromembryonicbrains,canformthetypicalneurospheresinsuspensioninvitroandhavetheabilityofmyelinating,andmaybepotentialsourcefortransplantationintreatingmyelindisorders.

简介：Mammaliantoothdevelopmentislargelydependentonsequentialandreciprocalepithelial-mesenchymalinteractions.Theseprocessesinvolveaseriesofinductiveandpermissiveinteractionsthatresultinthedetermination,differentiation,andorganizationofodontogenictissues.Multiplesignalingmolecules,includingBMPs,FGFs,Shh,andWntproteins,havebeenimplicatedinmediatingthesetissueinteractions.Transcriptionfactorsparticipateinepithelial-mesenchymalinteractionsvialinkingthesignalingloopsbetweentissuelayersbyrespondingtoinductivesignalsandregulatingtheexpressionofothersignalingmolecules.Adultstemcellsarehighlyplasticandmultipotent.Thesecellsincludingdentalpulpstemcellsandbonemarrowstromalcellscouldbereprogrammedintoodontogenicfateandparticipatedintoothformation.Recentprogressinthestudiesofmolecularbasisoftoothdevelopment,adultstemcellbiology,andregenerationwillprovidefundamentalknowledgefortherealizationofhumantoothregenerationinthenearfuture.

简介：骨头髓(BM)的细胞的基础织物开发和新生通过造血的干细胞(HSC)和间充质的干细胞(MSC)被调停。在造血的房间和BMstromal房间(BMSC)之间的本地相互影响在myelosuppression以后决定造血作用的体质。这里，我们在侮辱以后控制BM新生考察BM本地信号。造血的生长因素(HGF)和BMSC生产的cytokines是在BM造血作用的规定的主要因素。对在多重种类的早胚胎开发批评的Morphogens被加到HSC管理者的家庭，包括Wnt蛋白质，槽口ligands，BMP，和刺猬的家庭。HSC和BMSC的全球基因表示分析开始为两种房间类型揭示了基因的签名组。更重要地，生物化学、生物的结合的全球基因表示的分析在BM期间本地信号学习新生强烈建议了HGF和cytokines不能是为BM恢复的主要本地管理者，chemokines(SDF-1，FGF-4)并且angiogenic生长因素(VEGF--一，Ang-1)在myelosuppression以后在BM宪法起有启发性的作用。BM毒性的管理的一个新方向从BM再生管理者的鉴定正在出现。

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简介：BACKGROUND:Transplantedmononuclearcell(MNC)ofumbilicalbloodcansurviveincentralnervoussystem(CNS)ofhostthroughbloodbrainbarrier,differentiateintonervercells,migratetodamagedsiteandintegratemorphologicalstrucghandfunctionwithnervecellsofhostsoastoimprovedeficienciesofsensatoryfunction,motorfunctionandcognitivefunctionandinfluenceonstrokesequela.OBJECTIVE:Toobservetheveintransplantationofhumanumbilicalcordbloodstemcells(HUCBSC)forimprovingneurologicalfunction,limbfuntionandactivityofdailylivingofpatientswithstrokeandevaluatethereliability.DESIGN:Self-controlledstudy.SETTING:DepartmentofNeurosurgery,theSecondPeople'sHospitalofZhengzhouCity;Red-crossedBloodCenterofHenanProvince;DepartmentofNeurosurgery,theFistAffiliatedHospitalofZhenzhouUniversity.PARTICIPANTS:Atotalof10patientswithstokesequelawereselectedfromDepartmentofCerebralSurgery,theSecondPeople'sHospitalofZhengzhouCityfromApriltoDecember2005.Therewere9malesand1femaleagedfrom35to75yearswiththemeanageof56years.AllofthemwerediagnosedwithCTandMRIexaminationandcoincidencewithdiagnosticcriteriaofstrokeestablishedbytheFourthNationalAcademicMeetingforCerebrovascularDisease.Allpatientsprovidedinformedconsent.METHODS:80-140mLumbilicalbloodoftermbirthofnewbornwasselectedhermeticallyandmaintainedinsterileplasticbag.Andthen,thebloodwascentrifugatedatthespeedof1500r/minfor30minutesat22℃inordertoseparateMNC,i.e.,HUCBSC.Inaddition,afterfinaldiagnosisduringhospitalization,strokepatientswereperfusedwithHUCBSCthroughsuperficialveinofbackofthehand.Eachpatientwasaveragelypenfusedwith6portionsofHUCBSC(cellularnumbers≥1×108/portion)andtheintervalbetweeneachportionwas1-7dayswiththemeanintervalof4days.MAINOUTCOMEMEASURES:①Neurologicalfunctionofstrokepatientswasevaluatedwithneurologicalfunctiondeficiency(NFD)befor

简介：Thehedgehog-patched(hh-ptc)intercellularsignalingpathwayhasrecentlybeenshowntocontroltheproliferationofepithelialstemcellsinbothDrosophilaandVertebrated.MutantandectopicexpressionanalysesinDrosophilasuggestthattheHHproteindiffusesfromthesignalingcellstopromotetheproliferationofnearbyovariansomaticstemcellsbyantagonizingthesuppressionofitsreceptorPTCtowardstheCItranscriptionfactorinthestemcells.Consequently,thetranscriptionofCIdependentgenesleadstostemcellproliferation.Thisregulatorypathwayappearstofunctionalsoinvertebrates,wheredefectsinptccausebasalcellcarcinoma,tumorsofepidermalstemcellorigin.BasalcellcarcinomacanalsobeinducedbyectopicexpressionofSonichedgehog(shh)orGlil,thevertebratehomologofci.Thesestudiessuggesttheconservationofthehhsignalingpathwayincontrollingepithelialstemcelldivisionsamongdifferentorganisma.

简介：客观：为了探索可行性构造遗传工程人，神经干细胞(hNSCs)由lentivirus调停了多表示基因以便为针的绳索损害(SCI)的进一步的研究提供接枝来源。方法：从人的流产胎的大脑外皮的人的神经干细胞被孤立并且有教养，然后，基因被lentivirus修改两个都表示绿荧光蛋白质(GFP)和老鼠neurotrophin-3(NT-3)；转基因的表示被荧光显微镜，胎儿的老鼠的背面的根中心和槽污点的方法检测。结果：遗传工程hNSCs成功地被构造。所有在荧光显微镜下面表示了明亮绿的荧光的遗传工程hNSCs被观察。转基因的hNSCs的调节媒介能导致从背面的根中心(DRG)挥舞长出的神经突。遗传工程hNSCs表示了高级NT-3which能被使用槽污点检测。结论：遗传工程hNSCs调停了bylentivirus能被构造多成功地表示基因。

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简介：客观：为了由adipose-derivedstromal房间(ASC)在vitro导致adipocyte区别，与绿荧光灯的蛋白质(GFP)从转基因的老鼠收获了并且估计经由ASC的附件构造脂肪质的纸巾打字的可能性我骨胶原scaffolds.Methods：从GFP转基因的老鼠的腹股沟的胖垫被酶为ASC(主要文化)的隔离消化。在到ASC的三个段落的扩大以后，房间被ASC在vitro在为二个星期，和adipocyte区别中等的anadipogenic孵化被词法观察和油红估计染色的O。然后，他们被纳入为12个小时andco有教养的骨胶原脚手架，为2months由皮下注射的培植列在后面到裸体老鼠的背面的皮肤。最新形成的纸巾被检测由他染色。结果：有教养的主要干细胞是像成纤维细胞、显示出的活跃增长。在在anadipocyte区别媒介被孵化以后，在逐渐地并且最后积累的细胞质的类脂化合物微滴发展成成熟adipocytes，它出现了在染色的油红O积极。0。5厘米的~3new组织块在裸体老鼠的背面的皮下面被发现，它被荧光灯的观察作为成熟脂肪质的纸巾证实并且他染色。结论：ASC能成功地区分脂肪质的纸巾进成熟adipocytes，它作为intracytoplasmic类脂化合物微滴展出象adipocyte一样形态学和快车。它是与ASC和类型设计的脂肪质的纸巾的一个有效模特儿我骨胶原脚手架。

简介：骨头导出髓的间充质的干细胞(MSC)是为房间移植在临床的应用程序显示出一个重要潜力的pluripotent干细胞。在现在的论文，proteomic技术被用来接近与猪的骨头髓MSC联系的蛋白质侧面并且在5-azacytidine(5-aza)的效果上调查MSC蛋白质的规定。超过1,700蛋白质种类根据胶化分析与MSC被分开。与控制MSC介绍的表达式相比，有起来调整的11个蛋白质点并且26在5-aza-treated房间的蛋白质模式下面调整。21蛋白质的一个总数被MALDI-TOF-MS分析成功地识别，在哪个之中一些有趣的蛋白质，例如高山哈B-crystallin，在A2的附属建筑，和stathmin1，被报导了通过不同发信号的小径在房间增长和区别包含。我们的数据应该为MSC区别和apoptosis的未来学习是有用的。

简介：ObjectToinvestigatetheclinicalsignificanceofallogeneichematopoieticstemcelltransplantation(allo-HSCT)followingfludarabine(Flu)-basednomnyeloablativeconditioningregimen.Methods7patientswitholderageororgandysfunctionreceivedeitheroftwoFlu-basednonmyeloablativeconditioningprotocolsfollowedbyinfusionofgranulocytecolony-stimulatingfactor(G-CSF)mobilizedallogeneicperipheralblooclstemcells(PBSC).Cy-elosporincombiningmethotrexatewasusedasgraftvshostdisease(GVHD)prophylaxis.ResultsMinimalalloHSCTassociatedtoxicitywasfoundapartfrommucositis.Theallogeneicdonorengraftmentswereverifiedinallthepatients.Sixofseveneasessurvivedmorethan5months.AcuteGVHDoccurredinthreeofsevenpatientsincludingacaseofgrade1IGVHD.ConclusionTherapidengrafunentofallo-PBSCandgraftvsleukemia(GVL)effectscanbeobtainedbyFlu-basednomnyeloablativeconditioningregimen.Thismanagementissuitableforthepatientswhoaretoooldorhaveorgandysfunction.

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简介：Objective:Toinvestigatethedifferentiativecapabilityofadulthumanbonemarrowmesenchymalstemcells(BMSCs)intoSchwann-likecells.Methods:BonemarrowswereaspiratedfromhealthydonorsandmononuclearcellswereseparatedbyPercolllymphocytesseparationliquid(1.073g/ml)withcentrifugation,cellswereculturedinDMEM/F12(1:1)mediumcontaining10%fetalbovineserum(FBS),20ng/mlepidermalgrowthfactor(EGF)and20ng/mlbasicfibroblastgrowthfactor(bFGF).Cellsofpassage1wereidentifiedwithimmunocytochemistry.Conclusions:BonemarrowcontainsthestemcellswiththeabilityofdifferentiatingintoSchwann-likecells,whichmayrepresentanalternativestemcellsourcesforneuraltransplantation.

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