简介:摘要目的分析替莫唑胺(TMZ)干预Livin基因过表达的胶质瘤TJ905细胞及其干细胞模型后对细胞增殖活性的影响,以及对胶质瘤TJ905细胞和TJ905干细胞中Livin、Caspase-3/7的作用机制。方法使用免疫磁珠方法从胶质瘤TJ905细胞系中分选出CD133阳性胶质瘤干细胞,采用慢病毒过表达技术建立Livin过表达的细胞模型,使用不同浓度(0、25、50、100、200、400 μmol/L)的TMZ干预细胞模型48 h,采用CCK-8、流式细胞术、反转录PCR检测药物干预后细胞增殖活性、细胞周期及Livin、Caspase-3/7基因的表达量。结果(1)胶质瘤TJ905细胞中Livin基因表达量明显高于同源TJ905干细胞,差异有统计学意义(P<0.05);(2)TMZ能抑制胶质瘤TJ905细胞及其干细胞的增殖活性,降低增殖速度,并且随药物浓度的增加抑制作用逐渐增强;(3)TMZ能抑制Livin基因的表达,诱导Caspase-3/7的表达,并且随药物浓度的增加抑制作用逐渐增强,差异有统计学意义(P<0.05);(4)Livin基因转染后胶质瘤干细胞细胞周期G0/G1增加明显,而TJ905细胞周期则是G2/M期少量增加;TMZ干预细胞模型后,TJ905干细胞过表达组在S、G2/M停滞,对照组则是在S期停滞;TJ905细胞过表达组在S期停滞,对照组G2/M则是低浓度诱导停滞,随药物浓度增加其诱导作用减弱,差异均有统计学意义(P<0.05)。结论TMZ可以通过抑制Livin基因的表达、诱导Caspase-3/7的表达、调控细胞周期变化来降低细胞增殖活性,促进细胞凋亡。
简介:摘要:随着经济的增长以及人们生活水平的提高,人们对于电能的需求越来越大,如何在需求和供给之间维持平衡,不仅要关注变电站的建设情况,也要提升变电站设备的日常维修工作。只有保证日常维修以及规划性维修项目落到实处,才能充分发挥检修计划和方案的优势,更好地完成供电任务。本文首先对 35
简介:AbstractImportance:Within the coronavirus disease 2019 (COVID-19) global pandemic, more attention is warranted for whether this new infectious disease has unique manifestations in children.Objective:To retrospectively determine the epidemiological and clinical characteristics of 35 children with COVID-19 in Beijing, China.Methods:We collected data for 35 children diagnosed with COVID-19 who were admitted to Beijing Ditan Hospital from January 2020 to June 2020, and analyzed their epidemiological characteristics, clinical manifestations, laboratory examinations, chest imaging findings, treatments, and outcomes.Results:The children comprised 18 boys (51.4%) and 17 girls (48.6%) aged 6 months to 15 years. All patients had clear epidemiological history, with family clusters accounting for 28 cases (80.0%) and clear tracing of exposure to high epidemic areas in the remaining 7 cases (20.0%). Four (11.4%) patients were classified as asymptomatic, 17 (48.6%) as acute upper respiratory infection, and 14 (40.0%) as mild pneumonia, with no severe or critical cases. Clinical manifestations were mild, including fever in 18 (51.4%), cough in 14 (40.0%), and nausea and diarrhea in 7 (20.0%) patients. White blood cell count was mostly normal (26 cases, 74.3%) or decreased (7 cases, 20.0%); lymphocyte percentage was increased in 24 (68.7%); neutrophil percentage was decreased in 25 (71.4%); alanine aminotransferase was increased in 3 (8.6%); and serum potassium was decreased in 4 (11.4%). Time to negative viral nucleic acid testing was 2-42 days (mean: 14.0 ± 9.4 days). Chest imaging examination revealed that 20 patients (57.1%) had different forms of lung inflammation. Treatment was mainly isolation and nutritional support. Eleven patients were treated with interferon atomization inhalation. No patients required oxygen therapy. All 35 children were cured and discharged. Length of hospital stay was 9-54 days (mean: 25.4 ± 13.8 days). During regular follow-up after discharge, 5 children showed positivity again in the viral nucleic acid test and were re-hospitalized for observation and treatment. The mean length of re-hospitalization stay was 10.8 days.Interpretation:Children with COVID-19 mainly become infected within their family, and children of all ages are generally susceptible. The disease in children is mostly mild and the prognosis is good. Nucleic acid tests in some patients become positive again after discharge, suggesting that it is of great significance to carry out centralized isolation medical observations and active nucleic acid tests in close contacts for early detection of patients and routine epidemic prevention and control.
简介:摘要目的通过分析药物超敏反应综合征的临床特点,提高对药物超敏反应综合征的诊断和治疗的认识。方法选取2014年1月1日至2019年12月31日中南大学湘雅二医院皮肤科收治的35例药物超敏反应综合征患者进行临床分析总结。结果35例患者中,引起或可能引起药物超敏反应综合征的药物有10类,致病药物中以抗生素最多见,其次为别嘌呤醇、抗癫痫药、非甾体抗炎药。临床表现主要为皮疹、发热、淋巴结肿大、嗜酸性粒细胞明显增高、肝功能损害等。皮疹表现多样,65.71%(23/35)的患者表现为发疹样皮疹。主要治疗方法为系统使用糖皮质激素及免疫球蛋白,必要时可联合免疫抑制剂和血液灌流。结论如患者有明确的用药史,出现皮疹、高热、淋巴结肿大,检查结果出现嗜酸性粒细胞、转氨酶升高等,应高度怀疑药物超敏反应综合征,早期诊断及治疗预后较好。
简介:摘要目的分析缺血性结肠炎(IC)的临床特点,进一步提高该病诊断及治疗水平。方法回顾性分析吉林大学第二医院2014年1月至2018年12月确诊的IC患者35例的临床表现、发病危险因素、结肠镜及腹部CT检查结果,总结其临床特点。结果35例患者中有31例以突然发作的腹痛、鲜血便为主要表现。10例行腹部CT扫描的患者均见长范围肠壁增厚,厚度为(7.92±1.41)mm,增厚肠壁CT值明显下降,为(21.20±2.27)Hu;35例结肠镜检查中,累及降结肠、乙状结肠、横结肠、升结肠、全结肠者分别为42.86%、31.42%、20.00%、2.86%、2.86%,以左半结肠为主,可见簇状、条带状分布的黏膜红斑、瘀斑、糜烂,甚至溃疡;35例患者均未累及直肠。结论IC的典型表现为突然发作的腹痛及鲜血便,急诊腹部CT对该病的早期诊断有明显的提示价值,结肠镜检查是该病的主要的诊断方法。