简介:[摘要]在设计工作中有时会遇到建筑物基础不在同一标高,存在高差,且工程要求先施工较浅基础,经过计算对建筑物之间的距离提出最小要求成为方案设计时结构工程师的必要工作,本文结合工程实例提出最小距离的确定过程。
简介:AbstractBackground:Epithelial to mesenchymal transition (EMT) is a key process in determining distant metastasis and intra-hepatic dissemination of hepatocellular carcinoma (HCC). Follistatin (FST) family members are considered to be an attractive therapeutic targets and prognostic indicators in cancers. As a derivative of FST, Follistatin Like 5 (FSTL5) may play a similar role in HCC cells. This study aimed to investigate the expression and function of FSTL5 in HCC and its role in EMT.Methods:FSTL5, E-cadherin and vimentin in HCC, and paracancerous tissues were detected by immunohistochemistry. Correlation of FSTL5 expression with overall survival was assessed. The proliferation and invasion of HCC cell lines SK-Hep1 and MHCC-LM3 were analyzed by cell counting kit-8 and Transwell assays. The expression of FSTL5, E-cadherin, and vimentin in HCC cells was examined by polymerase chain reaction and Western blot analysis. T-test was used to analyze the difference in proliferation and invasion ability between groups. The Spearman rank correlation test was used to detect the correlation between the expression of FSTL5 and E-cadherin or vimentin.Results:The expression of FSTL5 in HCC was lower than that in paracancerous tissues (9.97% vs. 82.55%, χ2 = 340.15, P < 0.001). Patients with high FSTL5 expression had a better prognosis (χ2= 8.22, P= 0.004) and smaller tumor diameter (χ2 = 45.52, P < 0.001), less lymph node metastasis (χ2 = 5.58, P= 0.02), earlier tumor node metastasis stage (χ2 = 11.29, P= 0.001), a reduced number of tumors (χ2 = 5.05, P= 0.02), lower alpha-fetoprotein value (χ2 = 24.36, P < 0.001), more probability of hepatitis carrying (χ2 = 40.9, P < 0.001), and better liver function grade (χ2 = 5.21, P = 0.02). Immunohistochemistry showed that FSTL5 expression in HCC tissues was positively correlated with E-cadherin expression (r = 0.38, P < 0.001) and negatively correlated with vimentin expression (r = -0.385, P < 0.001). Furthermore, over-expression of FSTL5 up-regulated the expression of E-cadherin and down-regulated the expression of vimentin in SK-Hep1 (negative control [NC] vs. FSTL5-interfering group [Lv-FSTL5]: E-cadherin [t= 45.03, P < 0.001], vimentin [t= 67, P < 0.001]) and MHCC-LM3 (NC vs. Lv-FSTL5: E-cadherin [t = 50, P < 0.001], vimentin [t = 72.75, P < 0.001]) cells at mRNA level. The same as protein level. In addition, the over-expression of FSTL5 inhibited the proliferation (NC vs. Lv-FSTL5: SK-Hep1, 3 d [t = 7.324, P = 0.018], 4 d [t = 6.23, P = 0.021], 5 d [t= 10.21, P= 0.003]; MHCC-LM3, 3 d [t= 4.32, P= 0.037], 4 d [t= 7.49, P= 0.012], 5 d [t= 9.3661, P = 0.009]) and invasion (NC vs. Lv-FSTL5: SK-Hep1, t= 21.57, P < 0.001; MHCC-LM3, t= 18.04, P < 0.001) of HCC cells.Conclusions:Down-regulation of FSTL5 may contribute to EMT of HCC, and FSTL5 is a potential target in the treatment of HCC.
简介:摘要第5版WHO乳腺肿瘤分类已于2019年底正式出版,本文就新版分类中的主要更新内容进行解读。内容主要涉及新版WHO乳腺肿瘤分类的总体情况,以及浸润性癌、非浸润性癌、上皮-肌上皮肿瘤和纤维上皮性肿瘤内容的更新。
简介:【摘 要】语音作为语言的一个重要组成部分,是发展语言技巧的基础以及提高语言整体水平的关键因素。提高语音教学的效率是发展学生自主学习能力的途径之一。本文通过一节语音课剖析当下语音教学中存在的问题,提出改进的建议,从收集材料,解读发音和学后巩固等方面入手,反思课堂实施,构建基于美好教学愿景下的小学英语语音课堂。
简介:【摘要】 经过长期的学习研究和课堂实践,逐步探索出一条更适合我们学校实际的高效课堂模式——“ 5+1” 高效课堂教学模式。在我校的“ 5+1” 教学模式下,如何构建科学有效的评价机制,利用评价机制,提升师师互助学习、师生互动合作、生生小组合作的能力,提高教学质量,成为当前我校课改进程中亟待解决的问题。学生方面的评价机制 : 班级层面的学生学习过程评价机制 , 学校层面的学生学习过程评价机制 , 学校层面的学生学习成果评价机制 。 教师方面的评价机制 : 新生课堂常规培训达标课 , 展示课评比 ; 优质课评比 。