简介：摘要目的分析儿童急性偏瘫患者与正常对照之间血清成纤维细胞生长因子水平的差异。方法应用酶联免疫吸附双抗体夹心法(ELISA)检测52例儿童急性偏瘫患者（感染23例，颅脑损伤18例，脑血管11例）及50例健康对照者的血清b-FGF水平。结果疾病组和对照组血清b-FGF水平差异有统计学意义(P＜0.05)。不同病因的急性偏瘫患者组间血清b-FGF水平差异无统计学意义(P＞0.05)。结论儿童急性偏瘫患者血清b-FGF明显升高，恢复期更为显著，提示b-FGF等细胞因子可能在血管炎症反应/局部脑血管闭塞及血管闭塞后新生血管形成等儿童急性偏瘫的病理机制中具有重要作用。

简介：(FRS2)成纤维细胞生长因素(FGF)受体底层2是在FGF小径发信号的主要调停人。最近的研究在FRS2显示出那激活mitogen的蛋白质kinase(MAPK)phosphorylates丝氨酸和threonine残余，否定地影响FRS2的导致FGF的酷氨酸phosphorylation(PY)。几种刺激能导致FRS2的serine/threoninephosphorylation(PS/T)，显示FRS2可能为学习在表明小径的生长因素之间的串音是有用的。这里，我们报导FRS2的导致FGF的PY能被EGF合作刺激在PC12房间稀释；这禁止的效果能被U0126完全颠倒，MEK的一个禁止者。我们进一步在FRS2识别了ERK1/2-binding主题并且在FGF或EGF刺激之上产生了FRS2-3KL，变异的缺乏MAPK绑定和磅。不同野类型(WT)FRS2，FRS2-3KL的导致FGF的PY不能被EGF合作刺激，和更展出的FRS2-3KL-expressingPC12房间禁止响应FGF处理比FRS2-WT-expressing房间区分潜力。这些结果建议FRS2的PS/T由FRS2-MAPK否定规章的循环调停了可以作为从另外的小径把否定规章的信号集成到产生FGFR的信号transduction的一个分子的开关工作。

简介：AbstractFibroblast growth factor 21 (FGF21) is a fasting or stress inducible metabolic hormone produced mainly in the liver. It plays important roles in regulating both glucose and lipid homeostasis via interacting with a heterodimeric receptor complex comprising FGF receptor 1 (FGFR1) and β-klotho (KLB). For the past decade, great effort has been made on developing FGF21 derivatives or specific FGF21 receptor agonists into therapeutic agents for various metabolic disorders including type 2 diabetes (T2D), obesity, and more importantly, nonalcoholic fatty liver disease (NAFLD). Here we have reviewed FGF21 gene and protein structures, its expression pattern, cellular signaling cascades that mediate FGF21 production and function. We have then summarized the six clinical trials utilizing four FGF21 analogues. Finally, two recent literatures on the development of GLP-1 and FGF21 dual agonists were presented briefly.

简介：AbstractObjective:To explore the levels of fibroblast growth factor 23 (FGF23) during pregnancy and its relationship with intrauterine growth restriction (IUGR).Methods:Pregnant rats were classified into an ad libitum rat chow group (ad libitum rat chow, AD group, n = 25) and an undernutrition group (50% of their daily food requirement, UN group, n= 25). The levels of maternal serum FGF23, tissue homogenate FGF23, and bone gla protein in fetal rats, and placental FGF23 mRNA and protein expression were examined by enzyme-linked immunosorbent assay, real-time qPCR analysis respectively. Finally, the effect of recombinant FGF23 on the viability of MG-63 cells was determined by cell proliferation assay. Data were analyzed with independent two-tailed t test and one-way analysis of variance. Spearman rank-order correlation coefficients (continuous variables) was performed to determine the relationship of results.Results:The diet restriction induced IUGR in rat offsprings, and the UN group exhibited a significantly lower FGF23 level (P < 0.05, n= 5). The FGF23 level was increased and peaked in maternal serum on gestation day (GD) 15, but peaked in fetal and placenta on GD20. Moreover, the tissue homogenate levels of FGF23 and bone gla protein in fetal rats in both groups were positively correlated (r= 0.923, P < 0.05; r= 0.925, P < 0.05, respectively, n = 15), FGF23 was localized to both decidual and labyrinth zones, with remarkably higher expression on GD20, P < 0.05, n= 5. In vitro, recombinant human FGF23 enhanced MG-63 cell viability, P < 0.05, n= 5.Conclusion:Prenatal undernutrition could decrease the FGF23 expression in fetal rats caused by the mother through the placenta, and induced the IUGR and hindered the ossification. And the FGF23 levels are peaked on GD15 mother but peaked on GD20 placenta and fetuses, these might be associated with the over compensation of maternal placenta on GD20.

简介：AbstractIn the past decades, skeletal muscle has become the focus of numerous studies due to its potential physiological role as an endocrine organ secreting hundreds of myokines. Among these myokines, fibroblast growth factor 21 (FGF21) and irisin are novel hormone polypeptides sending signals to regulate the function of specific organs, like skeletal muscle, liver, pancreas, and adipose tissue. Both hormones have been reported to normalize glucose, improve insulin resistance, and promote lipid homeostasis, thereby preventing the development of metabolic disorders, such as obesity and diabetes. Besides preserving pancreatic β-cell functions, FGF21 also protects pancreatic acini from inflammation and reduces proteotoxic stress via facilitating digestive enzyme secretion. Meanwhile, irisin is found to inhibit the pancreatic cancer cell growth as well. This review attempts to focus on the current knowledge of FGF21 and irisin and their effective roles in pancreas including pancreatic β- and acinar cells under various physiological conditions, its anti-diabetic actions, and the clinical implications.

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简介：摘要急性肾损伤（AKI）在世界范围内具有较高的发病率和病死率，且是慢性肾脏病（CKD）和进展为终末期肾脏病的危险因素，给患者带来日益沉重的健康负担。成纤维生长因子23（FGF23）和a-Klotho（以下称“Klotho”）通路是近年来的研究热点。AKI发生后，血清中FGF23水平迅速上升，Klotho表达下降。这两种指标的变化被认为是预测AKI发生及预后的新型生物预测标志。本文围绕FGF23和Klotho在AKI中的表达改变和相关机制进行综述。

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简介：在历届全国各地的初中数学竞赛试题中，经常遇到有关化简形如(或可化为)式子√(a+√b)±√(a-√b)的问题．这类问题通常有下面三种化简方法，举例说明．

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简介：4月初参加了“西湖论坛电商好项目”的评委工作，这次活动不啻于医药互联网创业项目的一次小型巡礼。令我稍感意外的是，十几家参赛项目中有近一半是以零售药店为业务或服务对象的B端电商，如药药好、51健康、终端联盟、药便宜、药易购、沃奇医药商城、医诺方舟等。顿时恍觉医药电商的风向有变，B2B这次站在了风口。

简介：B2B是企业与企业之间的产品、服务及信息的交换，除了在线交易和产品展示，B2B的业务更重要的意义在于，将企业内部网，通过B2B网站与客户紧密结合起来，通过网络的快速反应，为客户提供更好的服务，从而促进企业的业务发展。

简介：虽然网络股一蹶不振,但电子商务的议题却始终是个热门话题。在所谓的B2C电子商务被投资人严厉质疑后,B2B则随着各大研究机构所勾勒的市场远景而成为下一波的抢手货。然而还记得在短短的两年前,我们对B2C电子商务的前景也有过极度乐观的预期,不是吗?现在却对之弃如蔽履。接着最近美国又有一些分析报告指出B2B的发展也存在着一些

简介：碱性成纤维细胞生长因子2（fibroblastgrowthfactor2,FGF2）是一类功能多样的多肽,可由视网膜的多种细胞分泌产生。随着对视网膜血管性疾病的研究深入,FGF2在其中的作用也得到广泛重视。FGF2可与血管内皮生长因子（vascularendothelialgrowthfactor,VEGF）、肿瘤坏死因子-α（tumornecrosisfactor-α,TNF-α）等细胞因子相互作用,通过发挥促血管生成作用、促炎作用以及一定的神经保护作用而参与视网膜血管性疾病的发生与发展。此外,FGF2的表达上调很可能是部分患者使用抗VEGF治疗效果不显著的重要因素。本文就FGF2的分子特征、分子作用机制及FGF2与视网膜血管性疾病的相关性进行总结,旨在为FGF2作为视网膜血管性疾病的治疗靶点提供理论依据。

简介：摘要2例FGF12基因变异所致早发型婴儿癫痫性脑病患儿就诊年龄分别为4月龄（男）和2月龄（女），分别于2018年9月和2019年4月收入广州市妇女儿童医疗中心，均为新生儿期出现反复抽搐，主要表现为癫痫发作形式多样，恶化期为痉挛后强直，伴或不伴痉挛发作，另伴有喂养困难和运动发育迟缓以及难治性肺炎。2例患儿均有FGF12基因杂合变异,变异位点分别为p.R52H和p.R114H。1例患儿因重症肺炎死亡，1例患儿加用钠离子通道阻滞剂后癫痫控制，运动发育进步，脑电图好转。

简介：摘要目的探讨血液净化技术治疗慢性肾衰对血清成纤维细胞生长因子（FGF23）、甲状旁腺激素（PTH）的影响作用。方法选取2015年1月—2015年12月在本院血液透析中心进行治疗的87例患者进行研究，根据治疗方式分为血液透析组29例，血液透析+血液透析滤过组33例，血液透析+血液灌流组25例。结果治疗8周后，HDF组和HP组患者的FGF23、PTH、BUN、Scr、β2-MG水平均低于HD组（P<0.05）。结论血液透析联合血液透析滤过或血液灌流能够更有效的降低患者血清FGF23、PTH水平。

简介：摘要目的研究静脉注射左卡尼汀对血液透析患者成纤维细胞生长因子(FGF)23水平的影响。方法将2017年１月至2018年1月我院60例维持性血液透析患者作为研究对象，随机分为治疗组30例和对照组30例，均行常规血透治疗，治疗组透析结束后给予左卡尼汀1.0g静脉注射，对照组不给予左卡尼汀。观察治疗后3个月、6个月两组患者FGF23水平。结果两组治疗前FGF23无统计学意义（P＞0.05）；治疗3个月、6个月后，实验组FGF23有明显的下降；结论维持性血透患者血透后静脉输注左卡尼汀，可以改善患者体内FGF23水平，影响患者的长期预后。

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简介：摘要对2020年2月在山东大学齐鲁儿童医院住院治疗的1例早发性癫痫性脑病患儿进行回顾性分析。患儿，女，4月龄时因"反复抽搐发作4个月、喂养困难1个月"入院。患儿出生1 d起病，发作类型为强直发作，发育严重落后，脑电图示多灶放电，后转为高度失律，头颅影像学阴性，3月龄时出现喂养困难。基因检测结果示FGF12基因新发杂合错义突变(Arg114His)。多种抗癫痫药物、生酮饮食治疗均无效，加用苯妥英钠后2个月未发作。患儿发作控制后可自行进食，但智力运动发育无进步。FGF12基因突变致早发性癫痫性脑病预后不佳，发作较难控制，应尽早应用苯妥英钠等钠离子通道阻滞剂。