简介：Objective:ToinvestigatetheeffectsofE7080andN5-(1-iminoethyl)-L-ornithinedihydrochloride(L-NIO)oncolorectalcanceraloneandincombination.Methods:HT29colorectalcancercelllinefromSapInstitutewasused.Real-timecellanalysis(xCELLigencesystem)wasperformedtodeterminetheeffectsofE7080andL-NIOoncolorectalcellproliferation.WhileapoptosiswasdeterminedwithAnnexinVstaining,andtheeffectofagentsonangiogenesiswasdeterminedwithchorioallantoicmembrane(CAM)model.Results:WefoundthatE7080hasastrongantiproliferativeeffectwithanhalfmaximuminhibitionofconcentration(IC50)valueof5.60×10–8mol/L.AlsoithasbeenobservedthatE7080showedantiangiogenicandapoptoticeffectsonHT29colorectalcancercells.AntiangiogenicscoresofE7080were1.2,1.0and0.6for100,10and1nmol/LE7080concentrations,respectively.Furthermore,apoptosishasbeendetectedin71%ofHT29colorectalcancercellsafteradministrationof100nmol/LE7080whichmayindicatestrongapoptoticeffect.MeanwhileadministrationofL-NIOalonedidnotshowanyeffect,butthecombinationofE7080withL-NIOincreasedtheantiproliferative,antiangiogenicandapoptoticeffectsofE7080.Conclusions:ResultsofthisstudyindicatethatE7080maybeagoodchoiceintreatmentofcolorectaltumors.FurthermoretheincreasedeffectsofE7080whencombinedwithL-NIOraisethepossibilitytousealowerdoseofE7080andthereforeavoid/minimizethesideeffectsobservedwithE7080.

简介：摘要目的将腹腔镜手术患者手术前后的血清作用于ECV304，观察细胞形态有无变化及合成eNOS的情况，探讨妇科腹腔镜手术是否会增加血管内皮细胞的损伤。方法将腹腔镜及开腹全子宫切除术患者(各8例)术前术后血清做为干预介质作用于ECV304，观察细胞形态的改变并用ELISA测定细胞液中eNOS含量变化。用SPSS17.0对检测结果分析。结果（1）不加干预血清的ECV304细胞培养2h、6h、12h、24h后，细胞液中eNOS的含量变化无统计学意义；（2）腔镜组及开腹组术前术后血清加入细胞液中培养2h、6h、12h、24h后，上清液中eNOS较干预前基础eNOS含量稍下降，差异无统计学意义；（3）将腔镜组手术前后血清配对，作用于ECV3042h、6h、12h、24h，比较其培养液中eNOS的含量，差异无统计学意义；(4)ECV304传代培养，细胞呈梭形和不规则三角形。经干预血清作用24h，显微镜下观察细胞形态，均有不同程度的细胞变圆及脱落。结论腹腔镜手术患者的血清作用于ECV304，对其分泌功能无明显影响，即对人体的内皮细胞无明显损伤。