简介:1.disapprove[dise'pru:v](v.)反对,不赞成;LindamarriedRoberteventhoughherparentsdisapproved.琳达·不顾父母的反对还是嫁给了罗伯特。2.ground[graund](a.)磨碎的;Ilovethesmellofgroundblackpepper.我喜欢黑胡椒粉的味道。3.exclude[ik'sklud](v.)把……排除在外Athleteswhotakeillegaldrugswillbeexcludedfromthecompetition.吃了禁药的运动员将不能参加比赛。
简介:1.Direction:Listentothedialogueandchoosethebestanswer.01TheSneeze1.WhereareDaisyandAlan?A.Onabus.B.Onametrotrain.C.Inthepark.2.WhydoesAlanarguewithDaisy?A.BecauseDaisy'sgradesarepoorthisterm.B.BecauseDaisytakesawayAlan'siPad.C.BecauseDaisyjustsneezedalloverAlan'sfaceandthepeoplearoundthem.3.Accordingtothedialogue,whichsentenceiscorrect?
简介:AIM:ToevaluatethecornealendothelialcelldensityandmorphologyinChinesepatientswithpseudoexfoliationsyndrome(PEX).·METHODS:Medicalrecordsof16patients(20eyes)withPEXwhopresentedtoourinstitutionbetweenJuly2008andJune2010wereretrospectivelyreviewed.Thirteeneyeshadcombinedglaucoma.Theinformationoffiveapparentlynormalfelloweyesinthesepatientswasalsorecorded.Lefteyesof20patientswithbilateralsenilecataractsbutnoothereyediseasewereincludedascontrols.Specularmicroscopywasperformedinalleyestoanalyzeforcornealendothelialcelldensityandmorphology.Celldensity,coefficientofvariationincellsize,andpercentageofhexagonalcellsincornealendotheliumwereevaluated.·RESULTS:ThemeancornealendothelialcelldensityinthePEXeyeswas2298±239cells/mm2,significantlylowerthanthatinthecataracteyes(2652±18cells/mm2,P=0.026),buttherewerenosignificantdifferencesincoefficientofvariationofcellsizeandfrequencyofhexagonalitybetweenthesetwogroups.NosignificantdifferencesinthethreeparameterswerefoundbetweentheapparentlynormalfelloweyesandthePEXeyesorthecataracteyes,orbetweenthePEXeyeswithandwithoutglaucoma.·CONCLUSION:CornealendothelialcelldensitymaydecreaseinChinesepatientswithPEX.ThedevelopmentofglaucomainPEXeyesdoesnotseemtoberelatedwiththechangeincornealendothelialcelldensityormorphology.
简介:AIM:Toinvestigatewhether15-Lipoxygenase-1(15-LOX-1)playsanimportantroleintheregulationofangiogenesis,inhibitinghypoxia-inducedproliferationofretinalmicrovascularendothelialcells(RMVECs)andtheunderlyingmechanism.METHODS:PrimaryRMVECswereisolatedfromtheretinasofC57/BL6JmiceandidentifiedbyanevaluationforFITC-markedCD31.ThehypoxiamodelswereestablishedwiththeBio-bagandevaluatedwithablood-gasanalyzer.ExperimentswereperformedusingRMVECstreatedwithandwithouttransferAd-15-LOX-1orAd-vectorbothunderhypoxiaandnormoxiaconditionat12,24,48,72hours.Theefficacyofthegenetransferwasassessedbyimmunofluorescencestaining.CellsproliferationwasevaluatedbytheCCK-8method.RNAandproteinexpressionsof15-LOX-1,VEGF-A,VEGFR-2,eNOsandPPAR-rwereanalyzedbyreal-timereversetranscriptionpolymerasechainreaction(RT-PCR)andWesternblot.RESULTS:RoutineevaluationforFITC-markedCD31showedthatcellswerepure.Theresultsofblood-gasanalysisshowedthatwhenthecultureswereexposedtohypoxiaformorethan2hours,thePo2was4.5to5.4Kpa.WeverifiedRMVECscouldbeinfectedwithAd-15-LOX-1orAd-vectorviaFluorescencemicroscopy.CCK-8analysisrevealedthattheproliferativecapacitiesofRMVECsinhypoxicgroupweresignificantlyhigherateachtimepointthantheywereinnormoxicgroup(P<0.05).Inahypoxiccondition,theproliferativecapacitiesofRMVECsin15-LOX-1groupweresignificantlyinhibited(P<0.05).Real-timeRT-PCRanalysisrevealedthattheexpressionsofVEGF-A,VEGF-R2andeNOsmRNAincreasedinhypoxiagroupcomparedwithnormoxiagroup(P<0.01).However,theexpressionsof15-LOX-1,PPAR-rmRNAdecreasedinhypoxiagroupcomparedwithnormoxiagroup(P<0.01).Italsoshowedthatinahypoxiccondition,theexpressionsofVEGF-A,VEGF-R2andeNOsmRNAdecreasedsignificantlyin15-LOX-1groupcomparedwithhypoxiagroup(P<0.01).However,15-LOX-1andPPAR-rmRNAincreasedsigni
简介:1七月份的欧洲,虽然炎热,但却并不过分。李蔚然坐在火车的小车厢里,吹着淡淡的冷风,望着车窗外不断掠过的山川和农田,已经有点昏昏欲睡了。史蒂夫坐在她对面的椅子上,正百无聊赖地翻看着一份薄薄的资料——李蔚然他们前几天接下的一份委托,调查一个叫做爱德华·海利斯的人——他的妻子怀疑他有婚外情。史蒂夫叹了口气,合上手中的文
简介:Adultstem/progenitorcellsplayimportantrolesintissuehomeostasisandhaveimportantimplicationsforregenerativemedicine.Itwasoncethoughtthatformationofnewbloodvesselsinadultonlyoccursthroughangiogenesis,aprocesswherebynewvesselsareformedfromexistingmatureendothelialcells;whilevasculogenesis,wherenewvesselsarederivedfromdifferentiationofendothelialprogenitorcells(EPCs),wasthoughttooccurexclusivelyinembryos.ThediscoveryofadultEPCsafewyearsagohaschangedthisoldparadigm;andsubsequentstudiesshowedthatEPCsmaybeapromisingtoolforthetreatmentofvasculardisorders.However,therehavebeenconflictingreportsonsubtypes,surfacemarkers,andfunctionsofEPCs;andthustheexactoriginandidentityofEPCsremaintobedefined.AcommonapproachtoobtainEPCsistoisolateandculturemononuclearcellsfromperipheralbloodandtoselectadherentcellsfor
简介:后来,赵玫说那天晚上什么事都没发生。关静民说,是吗?笑了笑,没说什么。其实他心里是不相信没发生什么事的。赵玫的家很空旷,莫名地弥漫着暧昧和不安。在赵玫平时和她丈夫睡觉的大床上,关静民把赵玫弄得欢欢畅畅,几次高声喊叫,惊得关静民停下动作,让她小声点儿,别让邻居听见。赵玫目光迷离,意识模糊,表情扭曲得像变了个人,陌生得很。赵玫的身体漂浮在半空,要升天了。关静民下了大力气(不下力气也不行,赵玫潮水般推着关静民,一点儿一点儿向大海深处漂去),脸上和身上都冒了汗,湿漉漉的。事毕,关静民歪到一边,点了支烟,吸了一口,又吸了一口,如释重负的样子。赵玫光着身子,歪着头,也不盖着点什么,晾着白肉,笑吟吟地看关静民。