简介:目的研究[^3H]洛非西定在大鼠体内不同给药途径的药代动力学。方法TLC法分离原型药物,液闪法洲定血浆中药物放射性强度,用3P87程序计算药动学参数。结果大鼠iv[^3H]OF其时-量曲线符合开放3室模型。T1/2为0.031-0.042h,T1/2(a)为0.352-0.398h,T1/2(B)为10.982-11.763h。大鼠po[^3H]LOF,其时-量曲线符合开放2室模型。T1/2(Ka)为1.037-1.102hT1/2(a)为2.113-2.325h,T1/2(B)为11.805-10.465h,Tmax为2.448-2.196h,F为53%55%。AUC和Cmax与剂量呈线性关系。结论药物在大鼠体内呈一级动力学过程。
简介:IthasbeenrevealedthatpolyⅠ:CisapotentstimulatorforNKcells,whichcaninduceNKcellrapidactivationandpreferentialaccumulationintoliver.However,theprocessmediatingtheinfluxofNKcellsremainsobscure.Inthisstudy,wefoundthatpolyⅠ:CadministrationincreasedtheportionandabsolutenumberofNKcellsinliver,butlargelydecreasedthoseinspleen.Therewerenoobviouschangesoftheselymphocytesinotherimmuneorgans.TheresultsfromsplenicadoptivetransferandsplenectomyshowedthattherecruitedspleenNKcellscontributedtotheaccumulationofNKcellsinliver,andthisprocesswasregulatedbytheproductionofchemokinesandthepresenceofTcells.Thisinvestigationwillhelptounderstandtheenhancedimmunecellrecruitmentinliveruponviralinfection.
简介:Objective:ToinvestigatetheexpressionofE2FandBcl-2andtheclinicopathologicalsignificanceinhepatocellularcarcinoma.Methods:TheexpressionsofE2F-3andBcl-2in74patientswithhepaticcarcinoma,paracarcinomaand15patientswithlivercirrhosisweredetectedbyS-Pimmunohistochemicalstaining.Results:TheexpressionofE2Finhepaticcarcinomawassignificantlyhigherthanthatinparacarcinomaorlivercirrhosis(P<0.005),theexpressionofBcl-2inhepaticcarcinomawassignificantlyhigherthanthatinparacarcinoma(P<0.005),inwhichBcl-2expressionwaslowerthaninlivercirrhosis(P<0.05).TheexpressionofE2F-3wasrelatedwithhistologicalgrade,tumorsize,andtheexpressionofBcl-2wasrelatedwithhistologicalgrade,tumorsizeandtumornumber.TherewascorrelationbetweentheexpressionofE2F-3andBcl-2inhepaticcarcinoma.Conclusion:E2F-3andBcl-2expressionmayplayanimportantroleindevelopment,progressionandcellapoptosisoftumor.
简介:目的:探讨VitaIn-Ceram渗透氧化铝、氧化锆陶瓷及EmpressⅡ铸瓷底层材料的可见光透射率,为临床选择核瓷材料及其颜色提供实验依据.方法:以Vitaalpha饰瓷材料为对照,采用分光光度计分别测量4种颜色的VitaIn-Ceram渗透氧化铝、4种颜色的氧化锆陶瓷、3种颜色的EmpressⅡ铸瓷底层材料的可见光积分透射率.结果:VitaIn-Ceram渗透氧化铝的透射率范围为1.434%~3.843%,氧化锆陶瓷的透射率范围为0%~0.587%,EmpressⅡ的透射率范围为5.620%~6.665%.结论:在3种牙科全瓷底层材料中,EmpressⅡ铸瓷材料的透射率最好,渗透氧化铝材料透射率较差,渗透氧化锆陶瓷材料的透射率最差.
简介:Objective:Toinvestigatetheexpressionofneurotrophin-3(NT-3)geneinSchwanncellsofratsciaticnerveintroducedbyanadenovirusvectorinvivo.Methods:ArecombinantadenovirusvectorforNT-3(Ad-NT-3)waspropagatedin293packagingcellsandtiteredwithtissuecultureinfectiousdose50(TCID50).Ad-NT-3wasinjecteddirectlyintotheratsciaticnerveaftertransectionandimmediaterepair.ImmunohistochemicalstainingwasemployedtodeterminetheexpressionofNT-3inSchwanncellsinratsciaticnerveandtheexpressiveintensityofthetissueslicesofthesciaticnervewasmeasuredwithLEICAM550imageanalysissystem.Results:Onthe2nddayafterinjectionofAd-NT-3,positivestainintheSchwanncellswasapparentinthevicinityofanastomosis.NT-3expressionincreasedsignificantlyonthe7thday(P<0.01)andthendecreased14-28daysafterinjection(P<0.01).TherewasnosignificantdifferenceofNT-3expressionbetweenthe14thand28thdaygroups(P>0.05).Comparedwiththe2nddaygroup,the14thand28thdaygroupsstillmaintainedarelativelyhighlevelofNT-3(P<0.01).Intactandrepairednerves,whichwereinjectedwithadenovirusencodingLacZgenes(Ad-LacZ)orphysiologicalsalineservedascontrols,showednoNT-3-positiveSchwanncells.Conclusions:AnadenovirusvectorcanbeusedtoinduceefficientlytheexpressionofNT-3geneinSchwanncellsofratperipheralnervesfollowingnerveinjuryandrepair,whichsuggeststhatneurotrophicfactorscanbeintroducedintoSchwanncellswithanadenovirusvectortopromoteperipheralnerveregeneration.
简介:USING3PRIMERPAIRSTODETECTHBVDNAINLIVERTISSUESFROMHEPATOCELLULARCARCINOMASWITHPCR TECHNIQUEChenMing陈明;LuLing吕凌;YaoJilu姚集鲁;Peng...