简介:建立了超高效液相色谱-高分辨质谱(ultraperformanceliquidchromatography-highresolutionmassspectrometry,UPLC-HRMS)快速筛查绿茶中2,4-滴、对氯苯氧乙酸、吲哚丁酸、氯吡脲和吲哚乙酸等12种植物生长调节剂的方法。采用CAPCELLPKA-C18色谱柱(100mm×2.1mm,2μm),正离子模式下以体积分数为5%的甲醇水溶液(含有5mmol/L乙酸铵和体积分数为0.1%甲酸)为流动相;负离子模式下以体积分数为5%甲醇水为流动相,梯度洗脱。在全扫描采集模式下,基于化合物的保留时间、准分子离子峰的精确质量数、碎片离子的精确质量数和同位素分布匹配指数,对目标物进行筛查分析。结果表明:12种植物生长调节剂在绿茶中的报告限(RL)为0.01mg/kg;在0.01、0.1和0.5mg/kg3个添加水平下,其回收率在61%~130%之间,相对标准偏差在1.8%~17%之间。该方法简便快捷,可用于绿茶中常用植物生长调节剂的筛查。
简介:为了研究M9矮化砧和八棱海棠砧YUCCA10a基因的结构特征及其嫁接树体的表达特征,从M9矮化砧和八棱海棠砧中克隆了YUCCA10a基因,并对该基因进行了序列分析以及不同时期2种砧木嫁接“烟富3”的定量表达分析。通过分析表明,该基因的编码序列为1149bp,推导编码382个氨基酸,预测蛋白质分子量为94285.29Da,理论等电点为5.08。实时荧光定量PCR结果表明,5月和10月份的M9矮化砧嫁接“烟富3”中YUCCA10a基因相对表达量明显低于八棱海棠砧,2种砧木嫁接的“烟富3”中YUCCA10a基因在10月份的相对表达量略高于5月份。本试验结果为研究M9矮化砧和八棱海棠砧YUCCA10a基因的结构特征和砧木对嫁接“烟富3”影响的机理方面提供理论依据。
简介:Background:Forestmanagementstrategiessuchasthinninghavelongbeenusedtoenhanceecosystemfunctions,especiallyinplantations.Thinninginplantationswithhighdeerdensity,however,maynotyieldadesiredincreaseinunderstoryvegetationbecausedeergrazeongerminatingplantsafterthinning.Here,weexaminethechangesinunderstoryvegetationafterthinninginplantationsthathavebeenovergrazedbysikadeertoprovideinsightintotheeffectsofthinningonecosystemfunctionssuchassoilconservationandbiologicaldiversity.Methods:WeconductedoursurveyintheTanzawaMountainsofeasternJapan.Wesurveyedthechangeinunderstoryvegetationwithinandoutsideofthreedeerexclosuresonasingleslopewiththreelevelsofunderstoryvegetationcover:sparse(1%,exclosure'US'),moderate(30%,exclosure'MM'),anddense(80%,exclosure'LD')over10yearsaftera30%thinningofanold-growthcedarandcypressplantationwhichwasovergrazedbysikadeer.Results:Understoryvegetationcover,biomassandspeciesrichnessincreasedwithinandoutsidethe'US'and'MM'exclosuresafterthinning,andbiomasswasgreaterwithinthanoutsidetheexclosuresat10yearsafterthinning.Unpalatablespeciesdominatedboth'US'and'MM'exclosuresbeforethinning,andtreesandshrubsdominatedwithintheexclosuresovertimeafterthinning.Incontrast,unpalatable,grazing-tolerant,perennial,andannualspeciesincreasedoutsidethe'US'and'MM'exclosures.Nonoticeablechangeswereobservedwithinandoutsidethe'LD'exclosurewhencomparedwiththe'US'and'MM'exclosures.Conclusions:Ourresultssuggestthatthinningastandby30%basedonvolumeresultedinanincreaseinunderstoryvegetationcovermainlycomposedofbothunpalatableandgrazing-tolerantspeciesinaplantationforestwhereunderstoryvegetationissparseormoderateandsikadeerdensityishigh.Weemphasizethatestablishingdeerexclosuresorcontrollingdeerisessentialtomaintainingsimilarunderstoryvegetationbothwit