简介：Objective:ToconstructsurvivinshRNAexpressionvectorcartingenhancedgreenfluorescentproteingene,transfectitintoGBC-SDHcellsviaelectroporation,andgetGBC-SDcellswhicharestableexpressingsurvivinshRNA.Methods:ThesiRNAsequencetargetingsurvivinmRNAwassynthesizedandclonedintopEGFP-H1.TheconstructedplasmidandpEGFP-H1weretransfectedintoGBC-SDcellsrespectivelyvialiposome,andthetransfectingeffectwasdetectedwithFlowCytometry.ThenthetransfectedcellswereselectedwithG418.Results:Therecombinantplasmidwassuccessfullyconstructed,namedpEGFP-survivin.ThegenetransfectionefficienciesinpEGFP-H1-transfectedgroupandpEGFP-survivin-transfectedgroupwerethe80.29%±2.71%and83.85%±2.34%(P>0.05),whichwassuccessfultogetthecellsthatarestableexpressingshRNA,namedGBC-SD/EGFPandGBC-SD/survivin.Conclusion:SurvivinshRNAexpressionvectorwasconstructedsuccessfullyandgotGBC-SDcellswhicharestableexpressionshRNA.