简介：FixedammoniumcontentsandNH4^+fixationcapacitiesofsomerepresentativecultivatedsoliscollectedfrom16provincesofChinawerestudied.Resultsshowedthatthecontentsoffixedammoniuminsoilsrangedfrom35to573mgNkg^-1,withanaverageof198mgNkg^-1.Thecontentoffixedammoniumcorrelatedverysignificantlywithmicacontentfortropicalandsubtropicalsoils,whereasthiswasnotthecaseforsoilsinthetemperatezone.AttheendofK-exhaustingexperimentthefixedammoniumcontentincreasedinmostsoilsstudied.However,itdecreasedinsmomtemperatesoils.Generally,fixationofaddedNH4^+couldnotbefoundeitherbeforeorafterK-exhaustingexperimentforhighlyweatheredsoils,includingtropicalsoilsandsoilsderivedfromgranite-gneissorQuaternaryredclaysinthesubtropiczone,whileformostsoilsintheYangtzeRiverdaltatheNH4^+fixationcapacitywasratherhighandincreasedsignificantlyintheK-exhaustedsoils.

简介：Aridsoilswherewaterandnutrientsarescarceoccupyover30%oftheEarth’stotalsurface.However,themicrobialautotrophyintheharshenvironmentsremainslargelyunexplored.Inthisstudy,theabundanceanddiversityofautotrophicbacteriawereinvestigated,byquantifyingandprofilingthelargesubunitgenesofribulose-1,5-bisphosphatecarboxylase/oxygenase(RuBisCO)formI(cbbL)responsibleforCO2fixation,inthearidsoilsunderthreetypicalplanttypes(Haloxylonammodendron,Cleistogeneschinensis,andReaumuriasoongorica)inNorthwestChina.Thebacterialcommunitiesinthesoilswerealsocharacterizedusingthe16SrRNAgene.Abundanceofred-likeautotrophicbacteriarangedfrom3.94×105to1.51×106copiesg-1drysoilandthoseofgreen-likeautotrophicbacteriarangedfrom1.15×106to2.08×106copiesg-1drysoil.Abundanceofbothred-andgreen-likeautotrophicbacteriadidnotsignificantlydifferamongthesoilsunderdifferentplanttypes.TheautotrophicbacteriaidentifiedwiththecbbLgeneprimerweremainlyaffiliatedwithAlphaproteobacteria,Betaproteobacteriaandanunculturedbacterialgroup,whichwerenotdetectedinthe16SrRNAlibrary.Inaddition,25.9%and8.1%ofthe16SrRNAgeneswereaffiliatedwithCyanobacteriainthesoilsunderH.ammodendronandR.soongorica,respectively.However,noCyanobacteria-affiliatedcbbLgenesweredetectedinthesamesoils.TheresultssuggestedthatmicrobialautotrophicCO2fixationmightbesignificantinthecarboncyclingofaridsoils,whichwarrantsfurtherexploration.

简介：PotexperimentswerecarriedouttoestimateN2fixationbyvetch,milkvetch,sicklealfalfaandbroadbeaninpurestandusinga^15N-labelledsoil.Winterwheatwasusedasthenon-fixingcontrol.The15N-labelledsoilusedwaspreparedbygrowingcorn-wheat-cornsuccessivelyonanearlyorganic-matter-freeXiashuloesssupplementedwithadequateamountsof(15NH4)2SO4,P,Kandmicronutrients,thenincorporatingthese15N-labelledplantmaterialsintothesoilaftereachhavest,andallowingtheplantmaterialstobedecomposedaerobicallyfor410dafterincorporationoftheplantmaterialofthethirecrop.The15Nenrichmentofwheatplant-Nvariedslightlywithorgans,withamaximumdifferenceof9.8%,Basedon15NenrichmentofsoilNinferredfromthemeanvalueofthe15Nenrichmentindifferentorgansofwheat79%-91%oftotalNinthetopsand67%-74%oftotalNintherootsoflegumesstudiedwerederivedfromatmosphere.EstimatebyisotopedilutionmethodwasingoodagreementwiththatbytheconventionaldifferencemethodprovidedvaluesobtainedbythelatterwerecorrectedforseedN,andalsowiththatfromthemeasurementofNaccumulatedinthetopsofthelegumes.