简介：BackgroundPostoperativewoundinfectionwasfrequentlyreportedinpatientsundergoingheartvalvereplacementandcoronaryarterybypasssurgery(CABG)duetomajortrauma,long-termbedrest,malnutritionandcompromisedimmunesystem.Infectedpatientswereusuallyhousedinthesameairborneinfectionisolationrooms.Aseriesofairmonitoringandcontrolstrategiesshouldbestrictlyenforcedtoaimatavoidingairbornefungalcontaminationandachievinghighercurerateininfectedpatients.TheefficacyofAirinspaceplasmairsysteminreducingairbornefungalcontaminationincardiacpostoperativeinfectionwardshasnotbeenclearlydetermined.MethodsAsurveyofaircontaminationwasconductedinthecardiacpostoperativeinfectionwardusingAirinspaceplasmairsystem.Laserparticlecounterwasusedtodeterminetheairborneparticlesof0.3μm?and0.5μm?insize.Airsampleswereincubatedandexaminedforfungaldevelopment.AirborneparticlecountsandfungalloadsofairsamplescollectedbeforeandaftertreatmentwithAirinspaceplasmairsystemwerecompared.ResultsTheparticlecountsin0.3μmrangecollectedbefore(52206<2345)andafter(9408<4317)treatedwithAirinspaceplasmairsystemdifferedsignificantly(P<0.01).Theparticlecountsin0.5μmrangebefore(12995<422)andafter(2016<915)treatmentwithAirinspaceplasmairsystemalsodifferedsignificantly(P<0.01).ThefungalloadsbeforeandafterusingAirinspaceplasmairsystemshowedsignificantdifference[(1975.3<356.1)cfu/m3vs.(193.83<29.5)cfu/m3,P<0.01].ConclusionAirinspaceplasmairsystemusedincardiacpostoperativeinfectionwardshasshownremarkableefficacyinreducingairborneparticlesandfungalcontaminationandhelpespreventcrossinfection.

简介：Thisstudyinvestigatedtheeffectofcatheter-basedrenalsympatheticdenervation(RD)onleftventricularhypertrophy(LVH)andsystolicanddiastolicfunctioninpatientswithresistanthypertension.LVHanddiastolicdysfunctionareassociatedwithelevatedsympatheticactivityandincreasedmorbidityandmortality.TheeffectofRDonLVHandLVfunctionisunclear.MethodsandResultsForty-sixpatientsunderwentbilateralRD,and18patientsservedascontrols.Transthoracicechocardiographywasperformedatbaseline,andafter1monthand6months.Besidesreductionofsystolicanddiastolicbloodpressure(-22.5/-7.2mmHgat1monthand-27.8/-8.8mmHgat6months,P<0.001ateachtimepoint),RDsignificantlyreducedmeaninterventricularseptumthicknessfrom14.1±1.9mmto13.4±2.1mmand12.5±1.4mm(P=0.007),andLVmassindexfrom53.9±15.6g/m(2.7)(112.4±33.9g/m(2))to47.0±14.2g/m(2.7)(103.6±30.5g/m(2))and44.7±14.9g/m(2.7)(94.9±29.8g/m(2))(P<0.001)at1monthand6months,respectively.ThemitralvalvelateralE/E'decreasedafterRDfrom9.9±4.0to7.9±2.2at1monthand7.4±2.7at6months(P<0.001),indicatingreductionofLVfillingpressures.Isovolumicrelaxationtimeshortened(baseline109.1±21.7msvs.85.6±24.4msat6months,P=0.006),whereasejectionfractionsignificantlyincreasedafterRD(baseline:63.1±8.1%vs.70.1±11.5%at6months,P<0.001).Nosignificantchangeswereobtainedincontrolpatients.ConslusionsBesidestheknowneffectonbloodpressure,ourstudyshowedforthefirsttimethatRDsignificantlyreducesLVmassandimprovesdiastolicfunction,whichmighthaveimportantprognosticimplicationsinpatientswithresistanthypertensionathighcardiovascularrisk.

简介：BackgroundOurpreviousstudyshowedthe150mg/mLfetalcardiacsupernatant(FCS)couldinducedifferentiationofBMSCsintocardiomyocye-likecellswithoutcardiomyocytetouch,butdifferentiationefficiencyisnothighenough.Inhibitionofglycogensynthasekinase-3enhancedtheproliferationandsurvivesofstemcells.Wetestedif6-bromoindirubin-3-oxime(BIO,glycogensynthasekinase-3inhibitor)enhancestheeffectsofFCSondifferentiationofBMSCsandexplorethegrowthfactorsinFCS.MethodsBMSCswereisolatedfromthefemurandtibiaoffour-week-oldmaleSprague-Dawleyratsandco-culturedwithFCS(150mg/mL)thatwasmadefromfetalheartsfromnineteen-daypregnantWistarrats.BIOwithdifferentconcentration(0,1,10,and100nM)wasintroducedinculturedishes.Transforminggrowthfactorbeta1(TGF-β1),bonemorphogeneticprotein2(BMP-2)andAktincardiacsupernatantandculturemediumwereassayedwithELISAmethods.ResultsAfterco-culturingwithFCS,beatingmyotubeswereobservedin25.9%BMSCsdishesafter1to2weeks’culture.ThelevelsofTGF-β1andBMP-2inFCSconcentrationswerenomorethanthatinyoungandadultcardiacsupernatant.AllBIOgroupssignificantlyenhancedtheeffectsofFCSondifferentiationofBMSCsintothecardiomyocyte-likecells(1nM,83%;10nM,73%;100nM,100%).AktlevelswerehigherinBMSCsculturalmediumwithFCS.ConclusionsFCScouldinducethedifferentiationofBMSCsintothecardiomyocyte-likecells.TGF-β1andBMP-2mightnotplayaroleinthedifferentiationofBMSCsinducedbyFCS.BIOenhancedtheeffectsofFCSonthedifferentiationofBMSCsintocardiomyocyte-likecells,whichmightinvolvetheAktpathway.