简介：VegetativecellsofPorphyrayezoensisareisolatedwithseasnailenzymeandculturedonthesolidifiedagarmedium.Theresultsofexperimentsshowthattheisolatedcellscansurvive,divideandregeneratewellonthemediumsolidifiedwithagar.Thefirstdivisiononthesolidmediumstartsafter7days'culture,4dayslaterthantheliquidculture.Thesurvivalrateofisolatedcellsis71.3%onthesolidmedium,lowerthanthe86.2%ofthatinseawater.Thalli,thalloids,conchocelis,spermatangiaandmulticellularmassesaredevelopedonthesolid/mediuminthefirstmonth,slowlybutnormally.Spermatangiasacsdisappearwithin4weeks.Withoutaddingnutrientliquidontothesurfaceofsolidmediumorinjectingseawaterundertheagarlayerinordertokeepmoisture,thethalliandcellgroupsreleasemonosporestoformnewthalliinsteadofenlargingtheirareasafter5weeks'culturing.Somemonosporesregeneratenewthalli.Othermonosporeslosetheirpigmentsandminimizetheirvolumeanddividequicklytoformlightpinkcalli.After16weeks,numerouscallicanbeseenonthesolidmediumandafter24weeks'culturing,almostonlycalliandconchoceliscanbeseen.Ifthecalliareimmersedinseawater,themonosporesarereleasedandmaydevelopintoyoungthallus.

简介：腐蚀抵抗无电在钠氯化物答案从12%～14%与磷的内容捏存款被学习。存款沉浸于3.5%NaCl答案让29d获得电气化学的参数；分别地为15d在标准盐水花测试被检验。存款的腐蚀抵抗被potentio动态的扫描学习，电气化学的阻抗光谱学(EIS)，X光检查衍射(XRD)；扫描电子显微镜学(FE-SEM)的冷地的排放与一个精力装备了散X光检查察觉者(EDX)。XRD的模式；FE-SEM的结果证明准备存款是非结晶的。但是在15d标准盐水花测试以后，一些针孔出现在存款的表面上；存款的表面上的磷的重量内容更高(它对钝化电影的形成有益)当因为镍的溶解重量比磷的大，镍内容更低时，比那，在标准盐前，水花测试。从potentio动态的扫描的结果；EIS证明钝化电影在NaCl答案在沉浸以后在Ni-P存款上形成了，它减少了腐蚀率捏样品。这个工作的结果在海洋的腐蚀显示出他们的潜在的应用。

简介：Harmfulalgalblooms(HABs)haveledtoextensiveecologicalandenvironmentalissuesandhugeeconomiclosses.VariousHABcontroltechniqueshavebeendeveloped,andbiologicalmethodshavebeenpaidmoreattention.Algicidalbacteriaisageneraldesignationforbacteriawhichinhibitalgalgrowthinadirectorindirectmanner,andkillordamagethealgalcells.AmetabolitewhichisstronglytoxictothedinoflagellateAlexandriumtamarensewasproducedbystrainDH46ofthealga-lysingbacteriumAlteromonassp.Thecultureconditionswereoptimizedusingasingle-factortestmethod.Factorsincludingcarbonsource,nitrogensource,temperature,initialpHvalue,rotationalspeedandsalinitywerestudied.Theresultsshowedthatthecultivationofthebacteriaat28℃and180rmin-1withinitialpH7and30saltcontcentrationfavoredboththecellgrowthandthelysingeffectofstrainDH46.TheoptimalmediumcompositionforstrainDH46wasdeterminedbymeansofuniformdesignexperimentation,andthemostimportantcomponentsinfluencingthecelldensityweretryptone,yeastextract,solublestarch,NaNO3andMgSO4.Whenthefollowingculturemediumwasused(tryptone14.0g,yeastextract1.63g,solublestarch5.0g,NaNO31.6g,MgSO42.3gin1L),thelargestbacterialdryweight(7.36gL-1)wasobtained,whichwasanenhancementof107%comparedtotheinitialmedium;andthealgallysisratewasashighas98.4%whichincreasednearly10%afteroptimization.

简介：Amutant(GQQ-M6)ofaSponge-Derivedstreptomycessp.GQQ-10obtainedbyUV-inducedmutationwasusedforproducingprodiginines(PGs).Singlefactorexperimentsandorthogonalarraydesign(OAD)methodswereemployedformediumoptimization.Inthesinglefactormethod,theeffectsofsolublestarch,glucose,soybeanflour,yeastextractandsodiumacetateonPGsproductionwereinvestigatedindividually.InthesubsequentOADexperiments,theconcentrationsofthese5keynutritionalcomponentscombinedwithsalinitywerefurtheradjusted.ThemutantstrainGQQ-M6gavea2.2-foldhigherPGsproductionthanthatoftheparentstrain;OADexperimentsofferedaPGsyieldof61mgL-1,whichwas10timeshigherthanthatoftheinitialGQQ-10strainundertheoriginalcultivationmode.