简介：Psoriasin(S100A7)是一种分子量为22.9kD的同源二聚体蛋白,属于S100蛋白家族成员。通过与钙离子结合,参与细胞内外重要的生命活动。其基因定位于染色体1q21.2-q22。Psoriasin(S100A7)蛋白最初是从银屑病异常增生的角化细胞中分离得到的,故称为Psoriasin,即银屑素。Psoriasin在异常增生的角化细胞、膀胱鳞状细胞癌和原位及浸润乳腺癌中表达上调。Psoriasin这种分泌和表达特征有可能作为膀胱鳞状细胞癌和乳腺癌等恶性肿瘤的候选标志物。

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简介：ThehistogensisofextramammaryPaget’sdiseasehasnotbeensolvedandremainedcontroversial.EightcasesofextramammaryPaget’sdiseaseofgeni-tocruralregionwereinvestigatedbyalcianblueandPASstainandimmunoreactionofanti-CEAandanti-keratin.Itwasfoundthatthepatternandintensityofalcianblue,PASstainingwereidenticalforPagetcellsandsecretorycellsofapocrinesweatgland;andCEAimmunoreactivitywasuniformlyobservedinbothPagetcellsandeccrinesweatgland.Thekeratinimmunoreactionwaspositiveinkeratinocytes,apocrineandeccrinesweatgland,whereasPagetcellswerenegative.TheseresultssuggestedthatPagetcellsofextramammaryPaget’sdiseasecouldbederivedfrommultipotentialepidermalgermcells.

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简介：Objective:Toinvestigatetheexpressionofmatrixmetalloproteinase-7(MMP-7)andFasligand(FasL)ingastriccancerandexploretheirroleinprogressionofgastriccancer.Methods:Formalin-fixedparaffinandembeddedtissuesofprimarygastriccancerandadjacentnon-tumormucosafrom113caseswereevaluatedforMMP-7,FasLandCapase-3expressionbystreptavidin-peroxidase(S-P)immunohistochemistry.Theexpressionofthefirsttwoproteinsincancercellsofprimaryfociwascomparedwithclinicopathologicalparametersoftumors.WealsoobservedthecorrelationofMMP-7andFasLexpressionwithCaspase-3expressionincancercellsofprimaryfoci.Results:MMP-7positiveimmunostainingwaslessfrequentlydetectedinadjacentepithelialcellsthanincancercellsofprimaryfociofgastriccancer(P<0.05,29.2%vs69.0%),andsowasFasL(P<0.05,34.5%vs54.0%).MMP-7expressionwasassociatedwithtumorsize,Borrmann'sclassification,invasivedepth,metastasisandTNMstaging(P<0.05),butnotwithgrowthpattern,Lauren'sclassification,orhistologicalclassification(P>0.05).FasLexpressionwascorrelatedwithtumorsize,invasivedepth,metastasis,Lauren'sclassification,histologicalclassification(P<0.05),whilenotwithBorrmann'sclassification,TNMstagingorgrowthpattern(P>0.05).CancercellsofprimaryfociexpressedlessCaspase-3thantheiradjacentepithelialcells(P<0.05,32.7%vs50.4%).TherewasanobviouscorrelationbetweenFasL,MMP-7andCaspase-3expressionincancercellsofprimaryfoci(P<0.05).Co-expressionofMMP-7andFasLparalleledwithCaspase-3expressionincancercellsofprimaryfoci(P<0.05).Conclusion:MMP-7andFasLexpressionwasup-regulatedingastriccarcinogenesisandwasprincipallyinvolvedinprogressionofgastriccancer.FasLexpressioncouldreflectthedifferentiationofgastriccancercellsandunderliethemolecularmechanismsofdifferentpathwaysofgastrictumorigenesis.Co-expressionofMMP-7andFasLcouldhaveapoptosis-inducingeffe

简介：Objective:TostudythesynergiceffectsofIL-12andB7-1transfectantonantitumorimmunityinvivo.Methods:TheretrovirusvectorencodingmIL-12andmB7-1genewastranfectedintoEL-4thymiclymphomacellsrespectively.Thecellswereusedastumorvaccineandthetherapeuticeffectwasobserved.Results:IncontrasttothemiceimmunizedwithEL-4/WtorEL-4/Neogroups,thetumorigenicityofEL-4/IL-12transfectantwasdecreased(P<0.001).TheEL-4/IL-12andEL-4/B7-1cellsirradiatedwith60CoshowedsignificantsystematicprotectiveeffectsagainsttherechallengeofEL-4/Wt.60CoirradiatedEL-4/IL-12cellsdelayedtheoccurrenceoftumorandprolongedthesurvivalperiodoftumorbearingmice.CombinationofthevaccinesofEL-4/IL-12andEL-4/B7-1resultedintheenhancedtherapeuticeffectcomparedwitheachsingletransfectantgroup(P<0.001).Conclusion:TheresultsshowedthatIL-12transducedcellscouldenhancetheantitumorimmunityofhostascancervaccine.CombinationoftheEL-4/IL-12andEL-4/B7-1transfectantcouldimproveimmunityofhostandisaprospectcancervaccine.

简介：Objective:Tostudythevaccinepotencyofgene-modifiedtumorcells.Methods:TheEL-4lymphomawastransducedwithrecombinantretroviruscontainingthemurineGM-CSFgeneorB7-1gene.Theeffectofgenetransductiononantitumorimmunitywasinvestigated.Results:Flowcytometryanalysisshowedthatexpressionoftheirsurfacemarkerbetweenwild-typeEL-4cellsandgenetransducedtumorcellswasthesameexceptforCD80positiveinB7-1genetransducedcells.GM-CSFgeneorB7-1genetransducedEL-4cellsresultedinremarkablelossoftumorigenicityinsyngeneticmice.ThesystemicprotectiveimmunitywasinducedagainstthechallengewithEL-4/wtcells.TherapeuticvaccinewithEL-4/GM-CSForEL/7-1cellscouldretardthegrowthofestablishedearly-stageEL-4/wttumorsignificantly,butnotretardthegrowthoflate-stageEL-4/wttumor.IrradiatedGM-CSFgenetransducedEL-4cellsshowedstrongvaccineeffectagainstEL-4cellchallenge,butirradiatedB7-1genetransducedEL-4cellsshowedweakvaccineeffect.RemarkablecooperativeantitumoreffectagainstEL-4cellchallengewasobservedwhenbothirradiatedEL-4/GM-CSFandEL-4/B7-1wereinoculatedtogether.Conclusion:GM-CSFgeneorB7-1genetransducedcombinationofthetwokindsofvaccinemayhavepotentialapplicationvalueinhumancancertreatment.

简介：目的:观察脐带血树突状细胞对小鼠S-180细胞株移植瘤的作用.方法:采用脐带血来源的单核细胞制备树突状细胞,体外经S-180细胞肿瘤相关抗原(TAA)致敏后免疫小鼠,一周后接种S-180细胞株.或先制备荷瘤小鼠模型,一周后再接种致敏树突状细胞.结果:脐带血来源的树突状细胞经胃癌细胞系S-180的TAA致敏后既能明显保护小鼠该移植瘤的发生,又能抑制已发生移植瘤的生长.同对照组相比,预防组和治疗组的瘤体积明显缩小,细胞免疫功能趋于正常,生存期延长(P＜0.05).结论:脐带血树突状细胞对小鼠S-180移植瘤的发生有预防作用,对荷瘤小鼠移植瘤的生长有抑制作用,为树突状细胞的临床应用提供了进一步的依据.

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简介：Objective:Toexplorethereversaleffectofmifepristoneonmultidrugresistance(MDR)indrug-resistanthumanbreastcancercelllineMCF7/ADRanditsmechanisms.Methods:ExpressionofMDR1andMDR-associatedprotein(MRP)mRNAinMCF7/ADRcellswasdetectedusingreversetranscription-polymerasechainreaction(RT-PCR).WesternblottingwasusedtoassaytheproteinlevelsofP-glycoprotein(P-gp)andMRP.Intracellularrhodamine123retentionand[3H]vincristine(VCR)accumulationweremeasuredbyflowcytometryandliquidscintillationcounter,respectively.MTTreductionassaywasusedtodeterminethesensitivityofcellstotheanticanceragent,adriamycin(ADR).Additionally,aMCF7/ADRcellxenograftmodelwasestablishedtoassessthereversaleffectofmifeprisoneonMDRinMCF7/ADRcellsinvivo.Results:Miferpristonedose-dependentlydown-regulatedtheexpressionofMDR1andMRPmRNAinMCF7/ADRcells,accompaniedbyasignificantdecreaseintheproteinlevelsofP-gpandMRP.Afterexposureto5,10,and20μmol/Lmifepristone,MCF7/ADRcellsshoweda3.87-,5.81-,and7.40-foldincreaseintheaccumulationofintracellularVCR(aknownsubstrateofMRP),anda2.14-,4.39-,and5.53-foldincreaseintheretentionofintracellularrhodamine123(anindicatorofP-gpfunction),respectively.MTTanalysisshowedthatthesensitivityofMCF7/ADRcellstoADRwasenhancedby7.23-,13.62-,and20.96-foldafterincubationwithmifepristoneasabove-mentioneddosesfor96h.Invivo,mifepristoneeffectivelyrestoredthechemosensitivityofMCF7/ADRcellstoADR.After8weeksofadministrationwithADR(2mg·kg-1·d-1)aloneorincombinationwithmifepristone(50mg·kg-1·d-1),thegrowthinhibitoryrateofxenograftedtumorsinnudemicewas8.08%and37.25%,respectively.Conclusion:MifepristoneexertspotentreversaleffectsonMDRinMCF7/ADRcellsinvitroandinvivothroughdown-regulationofMDR1/P-gpandMRPexpressionandinhibitionofP-gp-andMRP-dependentdrugefflux,thusincreasing

简介：客观：为了改进倔强的脸中部的外部T房间non-Hodgkin鈥檚的功效，有L天门冬氨酰胺酶(LASP)的淋巴瘤(MPTC-NHL)基于抢救化疗。方法：有倔强的MPTC-NHL的21个病人被分析，11patients(LASP组)收到了L天门冬氨酰胺酶基于的抢救化疗由L天门冬氨酰胺酶，长春新碱和dexame-thosone组成。没有L天门冬氨酰胺酶，10个病人(控制组)收到了抢救联合化疗。结果：完全的宽恕率为LASP组是45.6%，0.0%为控制组织(P<0.05)。全面反应率(CR+PR)为LASP组是63.6%，10.0%为控制组织，分别地(P<0.05)。2年的幸存率为LASP组是45.5%，0.0%为控制组织(P<0.05)。LASP的主要不利效果是白细胞减少，浆液bilirubin和多糖症的举起。结论：LASP基于的初步的临床的学习表演抢救化疗可以与倔强的MPTC-NHL改进反应率和病人的2年的幸存率。进一步继续学习是必要的。

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简介：目的构建细胞外基质蛋白1基因（ECM1）的真核表达载体ECM1-pEGFP-N2，检测其在人乳腺癌细胞系MCF-7中表达。方法采用PCR方法，以ECMleDNA为模板，扩增出ECM1基因，用Bg1Ⅱ和KpnⅠ双酶切ECM1基因和pEGFP-N2载体，连接酶切目的片段，转化大肠杆菌DH5，筛选阳性克隆酶切、测序鉴定；利用脂质体介导的转染技术转染MCF-7细胞，荧光显微镜检测报告基因表达产物EGFP，免疫组化检测ECM1蛋白表达。结果利用PCR克隆出约1．6kb的ECM1基因；PCR鉴定、酶切鉴定及测序结果证实成功构建ECM1-pEGFP-N2真核表达载体；转染MCF-7细胞后，可在细胞内观察到报告基因产生的绿色荧光，用免疫组化方法可检测到ECM1蛋白。结论成功构建了ECM1-pEGFP-N2真核表达载体，并在MCF-7细胞中表达，为进一步研究ECM1的功能奠定了基础。

简介：目的研究良恶性胸腔积液中间期细胞遗传学方面的差异,以及FISH技术对此种差异的鉴别能力.方法采用7号、8号人染色体着丝粒特异性探针对21例临床诊断明确患者的胸腔积液(11例肿瘤患者,10例非肿瘤患者)进行FISH分析,计数超二倍体细胞的比例,以正常人外周血淋巴细胞作为对照来判定染色体数目的异常,并将结果与临床诊断进行比较.结果在正常对照的淋巴细胞中,7、8号人染色体着丝粒探针各出现2个杂交信号,肿瘤患者胸腔积液细胞中7、8号人染色体数目的变化主要表现为拷贝数增多.在11例恶性胸腔积液中,出现7、8号人染色体数目增多的例数分别为8(72.7%)和9(81.8%),10例良性胸腔积液中7、8号人染色体为正常二倍体的各有9例(90.0%).结论7、8号人染色体超二倍体改变是肿瘤患者胸腔积液细胞的主要特征,采用染色体着丝粒特异性探针的荧光原位杂交技术能够检测到胸腔积液标本中的超二倍体肿瘤细胞,并且具有较好的性能.