简介：AbstractPurpose:The long-term situation of children with spinal cord injury (SCI) was investigated, and suggestions for helping them better return to the society were provided.Methods:SCI patients less than 18 years old hospitalized in Beijing Boai Hospital from January 2011 to December 2020 were retrospectively analyzed. Information including motor function, complications, characteristic changes, self-care abilities, school attendance and social participation were collected by telephone interview and electronic questionnaire. All the answers were statistically analyzed.Results:A total of 86 cases were enrolled, 77 girls and 9 boys, with a median injury age of 6 years and 2 months. The follow-up time was 3-130 months. The main cause of trauma in these children was sport injury (66.3%), the thoracic spinal cord was involved the most (91.9%), and complete SCIs accounted for the majority (76.7%). In terms of complications, children with complete SCIs were more likely to have urinary incontinence, constipation and characteristic changes (p < 0.05); whereas the incomplete SCIs often have spasticity (p < 0.05). As to the daily living abilities, children with incomplete lumbar SCIs were more capable to accomplish personal hygiene, transfer, and bathing independently than those with complete injuries, or cervical/thoracic SCIs, respectively (p < 0.05). Moreover, children older than 9 years care more able to dress and transfer independently than the youngers (p < 0.05). Wheelchair users accounted for 84.9% and more than half of them were able to propel wheelchair independently, and those who move passively in wheelchairs were mostly introverted kids (p < 0.05). Almost all (93.8%) children with incomplete injuries were able to walk independently. Most (79.1%) children continued to attending school, and 41.9% participated in interest classes. Unfortunately, 67.4% of the children spent less time playing with their peers than before the injury.Conclusion:SCIs impair physical structures and function of children, affect their independence in daily living, and restrict school attendance and social interaction. Comprehensive rehabilitation after injury is a systematic work. Medical staff and caregivers should not only pay attention to neurological function, but also help them improve self-care abilities. It is also important to balance rehabilitation training and school work and social participation.

简介：ObjectiveTo观察激活p21的kinase6(PAK6)在在成年rat.MethodsSprague-Dawley老鼠的针的绳索损害(SCI)以后的表示和它的可能的角色受到针的绳索损害。为了探索PAK6，表示模式和PAK6的分发的病理学、生理的意义，没被西方的污点观察，免疫组织化学和immunofluorescence.ResultsWestern污点分析证明PAK6蛋白质水平在白天2和白天4上是显著地起来调整的，然后减少了并且有到白天14为止的起来规定。免疫组织化学分析证明PAK6的表示显著地在与控制组相比的白天4上被增加。而且，染色的两倍immunofluorescence证明PAK6首先在控制组在神经原和星形细胞被表示。当时在损害以后，PAK6的表示在星形细胞显著地被增加，神经原，和星形细胞大部分被增殖。我们也检验了增殖的房间的表示原子抗原(PCNA)并且发现它的变化与PAK6的表示被相关。重要地，在在受伤针的绳索的PAK6的injury.ConclusionThe起来规定可以与glial增长被联系以后，染色的两倍immunofluorescence表明PCNA评估的房间增长在白天4上出现在许多PAK6-express-ing房间。

简介：Objective:Toexploretheepidemiology,clinicalpresentation,radiologyandsurgicaltreatmentoutcomeinChinesepatientswithmyelopathycausedbycontiguousmultilevelossificationofligamentumflavum.Methods:Medicalnotesandimagingdataof18Chinesepatients(14malesand4females,aged43-72years,mean:57years)withmyelopathycausedbycontiguousmultilevelossificationofligamentumflavumwerestudiedretrospectivelyinthisarticle.Thediagnosiswasbasedonclinicalexamination,X-rayfilms,computerizedtomography(CT)andmagneticresonanceimaging(MRI)scanningresultsandpathologicalresults.Sixteenpatientsweretreatedbylaminectomyandtwobylaminoplasty.Theaveragefollow-updurationwas34months(range,28-49months).TheoutcomewasevaluatedbyJapaneseOrthopaedicsAssociation(JOA)scope.Results:Theaveragetimeforoccurringclinicalsymptomswas7.5months(range,2days-16months).Allthe18casespresentedwithclinicalevidencesofchronicandprogressivethoracicspinalcordcompression,whichincludedbilaterallegweakness,spasticgait,numbnessinlowerlimbs,paresthesiainterminalandperineum,andurinaryincontinence.Neurologicalexaminationrevealedseverespasticparaparesis,absenceofabdominalreflexes,andreductionofthesensoryfunctionbelowthecompressionlevel.ThemeanJOAscorebeforeoperationwas3.6(range,0-6).MRIandCTscansofthethoracicspineconfirmedthepresenceofcontiguousmultilevelossificationoftheligamentumflavum.ThemeanrecoveryrateaftersurgeryintermsofJOAscorewas66.3%(range,33.3%-100%),withameanfinalJOAscoreof8.3.Thoracicdecompressionlaminectomyorlaminoplastycouldresultinagoodpostoperativeoutcome.Conclusions:ContiguousmultilevelossificationoftheligamentumflavumisnotacommoncauseofmyelopathyinChinesepopulationandshouldbetreatedasearlyaspossible.MRIandCTscanexaminationsmaydiagnosethepresenceofthoracicossificationofligamentumflavum(OLF).Posteriordecompression,especiallywit

简介：AbstractChemotherapy is often used for female malignancies, but it can increase the risk of premature ovarian failure in women of reproductive age through different mechanisms. Therefore, how to protect ovarian function and preserve fertility has attracted great attention of oncologists and gynecologists. Recently, umbilical cord mesenchymal stem cells (UCMSCs) have been extensively studied in the field of regenerative medicine. Compared with mesenchymal stem cells (MSCs) from other sources, UCMSCs have a broader application potential due to their properties of lower immunogenicity, fewer ethical issues, and non-invasive collection. Paracrine is one of the most important therapeutic mechanisms of UCMSCs, which can exert anti-inflammatory, anti-fibrosis, anti-oxidative stress, immune regulation, and other therapeutic effects. Studies in animal models have shown that UCMSCs can restore ovarian function after chemotherapy injury. However, most of the relevant researches are still in the preclinical stage. In this article, the mechanism of chemotherapy-induced ovarian failure will be overviewed, and the clinical application potential of UCMSCs in chemotherapeutic ovarian injury will be discussed.

简介：Toexplorethemolecularmechanismoftheprotectiveeffectofnervegrowthfactor(NGF)oninjuredspinalcord.Methods:TheposteriorT8(the8ththoracicsegment)spinalcordsof60Wistarratswereinjuredbyimpactscausedbyobjects(weighing10g)fallingfromaheightof2.5cmwithAllensway.Solutionwithnervegrowthfactors(NGF)wasgivento30rats(theNGFgroup)throughamicrotubuleinsertedintothesubarachnoidcavityimmediately,andat2,4,8,12and24hoursafterspinalcordinjury(SCI)respectively.Normalsaline(NS)withsamevolumewasgiventotheother30rats(theNSgroup)withthesamemethod.And5normalratsweretakenasthenormalcontrols.Theexpressionofbcl-2andbaxproteinsinspinalcordwasdetectedwithimmunohistochemistry.Theapoptoticneuronsinspinalcordweremeasuredwithterminaldeoxynucleotidyltransferase-mediateddUTP-biotinnickend-labelingofDNAfragments(TUNEL)staining.Results:Thepositiveexpressionofbcl-2proteinwasstronginthenormalcontrols,butdecreasedintheNSgroup,andincreasedsignificantlyintheNGFgroupascomparedwiththatoftheNSgroup(P<0.01).Thepositiveexpressionofbaxproteinwasalsostronginthenormalcontrols,butincreasedintheNSgroup,anddecreasedsignificantlyintheNGFgroupascomparedwiththatoftheNSgroup(P<0.01).ApoptoticneuronswerefoundintheNSgroup,andtheydecreasedsignificantlyintheNGFgroupascomparedwiththatoftheNSgroup(P<0.01).Conclusions:NGFcanprotecttheinjurednervetissuesthroughstimulatingtheexpressionofbcl-2protein,inhibitingtheexpressionofbaxproteinandinhibitingtheneuronalapoptosisafterSCI.

简介：RapamycintreatmenthasbeenshowntoincreaseautophagyactivityandactivateAktphosphorylation,suppressingapoptosisinseveralmodelsofischemiareperfusioninjury.However,littlehasbeenstudiedontheneuroprotectiveeffectsonspinalcordinjurybyactivatingAktphosphorylation.Wehypothesizedthatbotheffectsofrapamycin,theincreasedautophagyactivityandAktsignaling,wouldcontributetoitsneuroprotectiveproperties.Inthisstudy,acompressivespinalcordinjurymodelofratwascreatedbyananeurysmclipwitha30gclosingforce.Ratmodelswereintraperitoneallyinjectedwithrapamycin1mg/kg,followedbyautophagyinhibitor3-methyladenine2.5mg/kgandAktinhibitorIV1μg/kg.Westernblotassay,immunofluorescencestainingandterminaldeoxynucleotidyltransferase-mediateddUTPnickendlabelingassaywereusedtoobservetheexpressionofneuronalautophagymoleculeBeclin1,apoptosis-relatedmoleculesBcl-2,Bax,cytochromec,caspase-3andAktsignaling.OurresultsdemonstratedthatrapamycininhibitedtheexpressionofmTORininjuredspinalcordtissueandup-regulatedtheexpressionofBeclin1andphosphorylated-Akt.Rapamycinpreventedthedecreaseofbcl-2expressionininjuredspinalcordtissue,reducedBax,cytochromecandcaspase-3expressionlevelsandreducedthenumberofapoptoticneuronsininjuredspinalcordtissue24hoursafterspinalcordinjury.3-MethyladenineandAktinhibitorIVinterventionsuppressedtheexpressionofBeclin-1andphosphorylated-Aktininjuredspinalcordtissueandreducedtheprotectiveeffectofrapamycinonapoptoticneurons.TheaboveresultsindicatethattheneuroprotectiveeffectofrapamycinonspinalcordinjuryratscanbeachievedbyactivatingautophagyandtheAktsignalingpathway.

简介：Objective:Tobetterunderstandthecharacteristicsoftheneurogenicmotorevokedpotential(NMEP)beforeandafteracutespinalcordinjury.Methods:WerecordedandcharacterizedthespinalcordNMEPfrom48normalratsandfrom38ratswithspinalcordhemisectionlesion.SpinalcordNMEPswereelicitedbyapplyingarangeofcurrentintensitieswithbipolarmicroelectrodestimulitotheC4cordsegmentandrecordingtheresponsesfromsciaticnerveswithbipolarmicroelectrodesplacedintheneurilemma.Results:Theevokedpotentialsconsistedofthreestableandreproduciblenegativeandthreepositivepeaks.Themean±SDlatenciesofN1were2.89±0.22msontherightsideand2.89±0.24msontheleftside.Themeanconductionvelocitywas47.9m/s.Themean±SDamplitudesofN1were3.61±2.10μVontherightsideand3.83±2.32μVontheleftside.TheamplitudesofN1weresignificantlydifferentamongtheeightstimulusintensitygroups(rightside:F=2.22,df=7201,P=0.03;leftside:f=2.11,df=7206,P=0.04).Theamplitudewaslargestwhenthestimulusintensitywas1.1-2.5mA.ThelatenciesofN1werenotsignificantlydifferentamongtheeightstimulusintensitygroups(rightside:F=0.40,df=7201,P=0.9;leftside:F=1.20,df=7206,P=0.3).TheamplitudesandlatenciesofN2,N3werenotsignificantlydifferentamongtheeightstimulusintensitygroups.Therewerenosignificantchangesinlatencyandamplitudebetweentheleftandtherightsidenerveresponses.Thirty-eightratsundewentT9cordrightsidehemisection.Amongthem,20(53%),30(79%),and32(84%)ratscouldnotberecordedincorrespondingtoN1,N2,andN3,respectively,intheright-sidesciaticnerves;and13(79%),18(47%),and21(55%),incorrespondingtoN1,N2,andN3intheleft-sidesciaticnerves.Thelatencywassignificantlydelayedonthebothrightandleftsides.TheamplitudeN1wassignificantlydepressedonthebothsides,withN3significantlydepressedontherightsideandN2notsignificantlydepressed.Conclusions:TheamplitudeofNissignificantlydiffere

简介：Gastrodin,anactivecomponentoftallgastrodiatuber,iswidelyusedinthetreatmentofdizziness,paralysis,epilepsy,strokeanddementia,andexhibitsaneuroprotectiveeffect.AratmodelofspinalcordinjurywasestablishedusingAllen’smethod,andgastrodinwasadministeredviathesubarachnoidcavityandbyintraperitonealinjectionfor7days.Resultsshowthatgastrodinpromotedthesecretionofbrain-derivedneurotrophicfactorinratswithspinalcordinjury.Aftergastrodintreatment,themaximumangleoftheinclinedplanetest,andtheBasso,BeattieandBresnahanscoresincreased.Moreover,gastrodinimprovedneuraltissuerecoveryintheinjuredspinalcord.Theseresultsdemonstratethatgastrodinpromotesthesecretionofbrain-derivedneurotrophicfactor,contributestotherecoveryofneurologicalfunction,andprotectsneuralcellsagainstinjury.

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简介：BACKGROUND:Thestellateganglionblock(SGB)playsaprotectiveroleinfocalcerebralischemia/reperfusioninjury.ThehumanSGBcanbesimulatedbytransectionofthecervicalsympathetictrunk(TCST)inrats.OBJECTIVE:ToobservetheeffectsofTCSToninduciblenitricoxidesynthase(iNOS)levelsandcerebralinfarctvolumeinthehippocampusofratswithcerebralischemia/reperfusioninjury,andtoanalyzethemechanismofaction.DESIGN,TIMEANDSETTING:Acompletelyrandomized,controlled,neuropathologicalexperimentwasperformedattheInstituteofNeurologicalDisease,TaiheHospital,YunyangMedicalCollegebetweenMarchandSeptember2006.MATERIALS:Atotalof93Wistarrats,aged17-18weeks,ofeithergender,wereusedforthisstudy.2,3,5-triphenyltetrazoliumchloridewaspurchasedfromChangshaHongyuanBiologicalReagentCompany,China.RabbitiNOSantibodyandgoatanti-rabbitIgGantibodyweretheproductsofWuhanBosterBiologicalReagentCo.,Ltd.,China.METHODS:Tenratswererandomlyselectedforthesham-operatedgroup.Cerebralischemia/reperfusioninjurywasinducedbymiddlecerebralarteryocclusion(MCAO)usingthesuturemethodintheremainingrats.Fortysuccessfulratmodelswererandomlyandequallydividedintothefollowingtwogroups:(1)TCSTgroup:subsequenttoTCST,MCAOwasperformedfor2hours,followedby24hoursreperfusion;(2)modelgroup:ratsunderwentexperimentalproceduressimilartotheTCSTgroup,withtheexceptionofTCST.Ratsinthesham-operatedgroupweresubjectedtoexperimentalproceduressimilartothemodelgroup;however,thethreadwasonlyintroducedtoadepthof10mm.MAINOUTCOMEMEASURES:Following24hoursofreperfusion,functionalneurologicaldeficitswerescored.BraintissuesectionsfromtenratsofeachgroupwereusedtomeasurecerebralinfarctvolumebyTTCstaining.HippocampaltissuesectionsofanadditionaltenratsfromeachgroupwereusedtodetectiNOSlevelsusingthestreptavidin-peroxidaseimmunohistochemis

简介：BACKGROUND:Previousexperimentshaveconfirmedbonemorphogeneticproteins(BMPs)upregulatecholinergicexpressioninneuronsisolatedfromtheembryonicrathippocampusandcerebralcortex.Therefore,BMPscouldbeusefulfortreatingAlzheimer'sdiseaseandotherneurodegenerativediseases.OBJECTIVE:BMP-4wasinfusedintothehippocampaldentategyrusoffornix-fimbriatransectedratstotesttheeffectsofBMP-4oncholinergicexpressionindentategyrusneurons,andtoobservechangesinspatialmemorybehavior.DESIGN:Arandomizedcontrolledanimalexperiment.SETTING:DepartmentofNeurosurgeryandLaboratoryforCellBiology,InstituteofGeriatrics,GeneralHospitalofChinesePLA.MATERIALS:Twenty-sevenhealthyadultmaleSpragueDawley(SD)rats,weighing250-300g,wereprovidedbytheLaboratoryAnimalCenteroftheGeneralHospitalofChinesePLA.Reagents:BMP-4(B-2680,SigmaCompany)andcholineacetyltransferase(ChAT)antibody(AB5042,ChemiconCompany)wereusedinthisstudy.Equipments:aratstereotaxicinstrument(type:SN-2N,NarushigeGroup,Japan)andImage-prog-plusimageanalysissoftware(MediaCyberneticscompany,USA)wereusedinthisstudy.Theprotocolwascarriedoutinaccordancewithethicalguidelinesfortheuseandcareofanimals.METHODS:ThisexperimentwasperformedintheInstituteofGeriatrics,GeneralHospitalofChinesePLAbetweenJuly2004andMarch2005.Ratswererandomlydividedinto4groups:Alzheimer'sdiseasegroup(n=7),normalcontrolgroup(n=5),BMP-4-Alzheimer'sdiseasegroup(n=8),andmodelgroup(n=7).IntheAlzheimer'sdiseasegroup,thelefthippocampalfornix-fimbriaofratswastransectedtomimicAlzheimer'sdiseasesymptoms.IntheBMP-4-Alzheimer'sdiseasegroup,1μLBMP-4(10mg/L)wasperfusedintotheleftdentategyruswithamicroinjectorat1μL/min.Inthemodelgroup,1μLsalinewasperfusedintothesamepositionbythesamemethod.Twenty-eightdaysafterinjection,Morriswatermazetestwasperformedinallr

简介：BACKGROUND:Stellateganglionblock(SGB)playsaprotectiveroleonthebrain,buttheprecisemecha-nismofactionisnotclear.OBJECTIVE:TosimulateSGBbytransectionofthecervicalsympathetictrunk(TCST)andtoinvestigatetheTCSTeffectsonchangesincerebralinfarctvolumeandoxygenfreeradicallevelsinratswithfocalcere-bralischemia/reperfusioninjury.DESIGN,TIMEANDSETTING:AcompleterandomizedcontrolanimalexperimentwasperformedattheInstituteofNeurologicalDiseasesofTaiheHospital,YunyangMedicalCollegefromFebruarytoDecember2005.MATERIALS:Atotalof101healthyWistarrats,weighing280–320g,ofbothgenders,aged17–18weeks,wereusedinthisstudy.2,3,5-triphenyltetrazoliumchloride(TTC)waspurchasedfromChangshaHongyuanBiologicalCompany.Superoxidedismutase(SOD),malondialdehyde(MDA)andnitricoxide(NO)assaykitswereprovidedbyNanjingJianchengBioengineeringInstitute.METHODS:RatswererandomlydividedintoaTCSTgroup,amodelgroupandashamoperationgroup.Successfulmodelswereincludedinthefinalanalysis,withatleast20ratsineachgroup.AfterTCST,ratmodelsoffocalcerebralischemia/reperfusioninjurywereestablishedintheTCSTgroupbyreceivingmiddlecerebralarteryocclusion(MCAO)bytheintraluminalsuturemethodfor2hours,followedby24hoursofreperfusion.Ratmodelsoffocalcerebralischemia/reperfusioninjuryweremadeinthemodelgroup.Ratsintheshamoperationgroupunderwentexperimentalproceduresasforthemodelgroup,threadingdepthof10mm,andmiddlecerebralarterywasnotligated.MAINOUTCOMEMEASURES:BraintissuesectionsoftenratsfromeachgroupwereusedtomeasurecerebralinfarctvolumebyTTCstaining.BraintissuehomogenateofanothertenratsfromeachgroupwasusedtodetectSODactivities,MDAcontentsandNOlevels.Ratneurologicalfunctionwasassessedbyneu-robehavioralmeasures.RESULTS:CerebralinfarctvolumewasbiggerinthemodelgroupthanintheTCSTgr

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简介：OBJECTIVE:Theaimofthisstudywastoevaluatetheeffectivenessandsafetyofstemcelltransplantationforspinalcordinjury(SCI).DATASOURCES:PubMed,EMBASE,Cochrane,ChinaNationalKnowledgeInfrastructure,ChinaScienceandTechnologyJournal,Wanfang,andSinoMeddatabasesweresystematicallysearchedbycomputertoselectclinicalrandomizedcontrolledtrialsusingstemcelltransplantationtotreatSCI,publishedbetweeneachdatabaseinitiationandJuly2016.DATASELECTION:RandomizedcontrolledtrialscomparingstemcelltransplantationwithrehabilitationtreatmentforpatientswithSCI.Inclusioncriteria:(1)PatientswithSCIdiagnosedaccordingtotheAmericanSpinalInjuryAssociation(ASIA)InternationalstandardsforneurologicalclassificationofSCI;(2)patientswithSCIwhoreceivedonlystemcelltransplantationtherapyorstemcelltransplantationcombinedwithrehabilitationtherapy;(3)oneormoreofthefollowingoutcomesreported:outcomesconcerningneurologicalfunctionincludingsensoryfunctionandlocomotorfunction,activitiesofdailyliving,urinationfunctions,andseverityofSCIoradverseeffects.Studiescomprisingpatientswithcomplications,withoutfull-text,andpreclinicalanimalmodelswereexcluded.QualityoftheincludedstudieswasevaluatedusingtheCochraneriskofbiasassessmenttoolandRevManV5.3software,providedbytheCochraneCollaboration,wasusedtoperformstatisticalanalysis.OUTCOMEMEASURES:ASIAmotorscore,ASIAlighttouchscore,ASIApinprickscore,ASIAimpairmentscalegradingimprovementrate,activitiesofdailylivingscore,residualurinevolume,andadverseevents.RESULTS:Tenstudiescomprising377patientswereincludedintheanalysisandtheoverallriskofbiaswasrelativelylowlevel.Fourstudiesdidnotdetailhowrandomsequencesweregenerated,twostudiesdidnotclearlystatetheblindingoutcomeassessment,twostudieslackedblindingoutcomeassessment,onestudylackedfollow-upinformation,andfourstudiesc

简介：BACKGROUND:Studieshavedemonstratedthatthemechanismsunderlyingcellularapoptosissignaltransductionfocusontwopathways:intracellularmitochondriaandextracellulardeathreceptor.Thecurrentevidencesupportsthatsignaltransductionofcellularapoptosisalsoincludesendoplasmicreticulumstresssignaltransduction.OBJECTIVE:ToobserveCaspase-12expressionandcellularapoptosisfollowingischemiainratswithprogressivespinalcordcompression,andtoverifytheinfluenceofendoplasmicreticulumstressontheapoptosisinducedbyspinalcordinjury.DESIGN,TIMEANDSETTING:Arandomized,controlled,animaltrialwasperformedattheInstituteofNeuroscienceinChongqingMedicalUniversitybetweenJanuaryandOctoberin2006.MATERIALS:Immunohistochemicalkit,diaminobenzidine,andTUNELkitwerepurchasedfromBeijingZhongshanBiotechnology,China;rabbitanti-ratCaspase-12monoclonalantibodywasprovidedbySantaCruz,USA.METHODS:SixtyWistarrats,aged3-4months,wererandomlyassignedtoamodelgroup(n=50),whichunderwentspinalcordcompressionintheL_1segmentfollowingL_1laminectomyandarticularprocessexcisiontoestablishamodelofprogressivespinalcordcompression,andasham-surgerygroup(n=10),whichunderwentonlylaminectomy.Startingwiththefirstdayaftersurgery,theratswerelocallyanesthetized,theskinwasopened,andthescrewwasrotatedby1/4ofacycle,twiceweekly.MAINOUTCOMEMEASURES:At3,7,14,21,and28daysaftersurgery,ratsfromeachgroupwereanesthetized,andthespinalcordswereresected.Pathologicalchangesfollowingspinalcordcompressionweredeterminedusinghematoxylin-eosinstaining,Nissldye,andtransmissionelectronmicroscopy.TheTUNELmethodwasusedtoobserveneuronalapoptosisinthecompressedspinalcordsegments.ImmunohistochemistryandWesternblotwereutilizedtodetectCaspase-12expressioninthecompressedsegments.RESULTS:Cellularswelling,neuraldegeneration,andalteredendoplasmicreticulumstructureswereobservedat3days

简介：Objective:Toobservetheeffectsofcryopreservedolfactoryensheathingcells(OECs)transplantationonaxonalregenerationandfunctionalrecoveryfollowingspinalcordinjuryinadultrats.Methods:Twenty-fourratsweredividedintoexperimentalandcontrolgroups,eachgrouphaving12rats.ThespinalcordinjurywasestablishedbytransectingthespinalcordatT10levelwithmicrosurgeryscissors.OECswerepurifiedfromSDratolfactorybulbandculturedinDMEM(Dulbecco'sminimumessentialmedium)andcryopreserved(-120℃)fortwoweeks.OECssuspension[(1-1.4)×105/ul]wastransplantedintotransectedspinalcord,whiletheDMEMsolutionwasinjectedinsteadinthecontrolgroup.At6and12weeksaftertransplantation,theratswereevaluatedwithclimbingtestandMEP(moterevokedpotentials)monitoring.Thesamplesofspinalcordwereprocuredandstudiedwithhistologicalandimmunohistochemicalstainings.Results:At6weeksaftertransplantation,alloftheratsinbothtransplantedandcontrolgroupswereparaplegic,andMEPscouldnotberecorded.MorphologyoftransplantedOECswasnormal,andOECswereinterfusedwithhostwell.Axonscouldregrowintogaptissuebetweenthespinalcords.BothOECsandregrownaxonswereimmunoreactiveforMBP.Noregrownaxonswerefoundinthecontrolgroup.At12weeksaftertransplantation,2rats(2/7)hadlowerextremitiesmusclecontraction,2rats(2/7)hadhipand/orkneeactivemovement,andMEPof5rats(5/7)couldberecordedinthecalfinthetransplantationgroup.Noneoftherats(7/7)inthecontrolgrouphadfunctionalimprovement,andnonehadMEPsrecorded.Inthetransplantedgroup,histologicalandimmunohistochemicalmethodsshowedthenumberoftransplantedOECsreducedandsomeregrownaxonshadreachedtheendoftransectedspinalcord.However,noregrownaxonscouldbeseenexceptscarformationinthecontrolgroup.Conclusions:CryopreservedOECscouldintegratedwiththehostandpromoteregrowingaxonsacrossthetransectedsp

简介：BACKGROUND:Transplantedmononuclearcell(MNC)ofumbilicalbloodcansurviveincentralnervoussystem(CNS)ofhostthroughbloodbrainbarrier,differentiateintonervercells,migratetodamagedsiteandintegratemorphologicalstrucghandfunctionwithnervecellsofhostsoastoimprovedeficienciesofsensatoryfunction,motorfunctionandcognitivefunctionandinfluenceonstrokesequela.OBJECTIVE:Toobservetheveintransplantationofhumanumbilicalcordbloodstemcells(HUCBSC)forimprovingneurologicalfunction,limbfuntionandactivityofdailylivingofpatientswithstrokeandevaluatethereliability.DESIGN:Self-controlledstudy.SETTING:DepartmentofNeurosurgery,theSecondPeople'sHospitalofZhengzhouCity;Red-crossedBloodCenterofHenanProvince;DepartmentofNeurosurgery,theFistAffiliatedHospitalofZhenzhouUniversity.PARTICIPANTS:Atotalof10patientswithstokesequelawereselectedfromDepartmentofCerebralSurgery,theSecondPeople'sHospitalofZhengzhouCityfromApriltoDecember2005.Therewere9malesand1femaleagedfrom35to75yearswiththemeanageof56years.AllofthemwerediagnosedwithCTandMRIexaminationandcoincidencewithdiagnosticcriteriaofstrokeestablishedbytheFourthNationalAcademicMeetingforCerebrovascularDisease.Allpatientsprovidedinformedconsent.METHODS:80-140mLumbilicalbloodoftermbirthofnewbornwasselectedhermeticallyandmaintainedinsterileplasticbag.Andthen,thebloodwascentrifugatedatthespeedof1500r/minfor30minutesat22℃inordertoseparateMNC,i.e.,HUCBSC.Inaddition,afterfinaldiagnosisduringhospitalization,strokepatientswereperfusedwithHUCBSCthroughsuperficialveinofbackofthehand.Eachpatientwasaveragelypenfusedwith6portionsofHUCBSC(cellularnumbers≥1×108/portion)andtheintervalbetweeneachportionwas1-7dayswiththemeanintervalof4days.MAINOUTCOMEMEASURES:①Neurologicalfunctionofstrokepatientswasevaluatedwithneurologicalfunctiondeficiency(NFD)befor

简介：Spinalcordinjury(SCI)isadevastatingconditionthatnotonlyresultsinalossofmotorfunctionsbutalsosevereautonomicdysfunctions(KrassioukovandClaydon,2006).Autonomicdysreflexia(AD)isalifethreateningepisodeoftransienthypertensionthatoccursupto30x/day(11x/dayon

简介：Objective:Todetecttheconcentrationofmonocytechemoattractantprotein-1(MCP-1)intheserumofpatientswithincompletespinalcordinjuryandevaluateitsrelationwiththepathologicclassificationofthespinalcordinjury.Methods:MCP-1concentrationintheserumofpatientswithincompletespinalcordinjury(iSCI),singlespinecompressionandhealthysubjectsweredetectedbyELISA,respectivelyinthepresentstudyandthemagneticresonanceimagingdataofthesepatientswerestudiedatthesametimeonablindbase.Results:SerumlevelofMCP-1iniSCIpatientswas428pg/ml±11pg/mlbyELISA,whichwashigherthanboththatofthepatientswithsinglespinecompressionandofcontrols,withtheconcentrationof184pg/ml±21pg/mland124pg/ml±15pg/ml,respectively.Therewassignificantdifferencebetweenanytwogroups(P<0.01).iSCIpatientswithnormalMRIshowedalowerserumlevelofMCP-1as312pg/ml±30pg/ml.Pathologicalclassificationofspinalcordedemaandhematomacorrespondedto390pg/ml±16pg/mland508pg/ml±24pg/mlintheconcentrationofMCP-1.Conclusions:MCP-1mayinducesecondaryinflammatoryresponsebyrecruitinginflammatorycellstotheinjurysiteandthusaffecttheprognosisofspinalcordinjury.

简介：Somestudieshavesuggestedthatearlysurgicaltreatmentcaneffectivelyimprovetheprognosisofcervicalspinalcordinjurywithoutradiologicalabnormality,butnoresearchhasfocusedonthedevelopmentofaprognosticmodelofcervicalspinalcordinjurywithoutradiologicalabnormality.Thisretrospectiveanalysisincluded43patientswithcervicalspinalcordinjurywithoutradiologicalabnormality.Sevenpotentialfactorswereassessed:age,sex,externalforcestrengthcausingdamage,durationofdisease,degreeofcervicalspinalstenosis,JapaneseOrthopaedicAssociationscore,andphysiologicalcervicalcurvature.Amodelwasestablishedusingmultiplebinarylogisticregressionanalysis.Themodelwasevaluatedbyconcordantprofilingandtheareaunderthereceiveroperatingcharacteristiccurve.Bootstrappingwasusedforinternalvalidation.Theprognosticmodelwasasfollows:logit(P)=-25.4545+21.2576VALUE+1.2160SCORE-3.4224TIME,whereVALUEreferstothePavlovratioindicatingtheextentofcervicalspinalstenosis,SCOREreferstotheJapaneseOrthopaedicAssociationscore(0–17)aftertheoperation,andTIMEreferstothediseaseduration(frominjurytooperation).Theareaunderthereceiveroperatingcharacteristiccurveforallpatientswas0.8941(95%confidenceinterval,0.7930–0.9952).Threefactorsassessedinthepredictivemodelwereassociatedwithpatientoutcomes:agreatextentofcervicalstenosis,apoorpreoperativeneurologicalstatus,andalongdiseaseduration.Thesethreefactorscouldworsenpatientoutcomes.Moreover,thediseaseprognosiswasconsideredgoodwhenlogit(P)≥-2.5105.Overall,themodeldisplayedacertainclinicalvalue.ThisstudywasapprovedbytheBiomedicalEthicsCommitteeoftheSecondAffiliatedHospitalofXi’anJiaotongUniversity,China(approvalnumber:2018063)onMay8,2018.