简介:Inthisstudy,threecDNAsequencescorrespondingtocytochromeP450C17(CYP17I),3β-hydroxysteroiddehydrogenase(3β-HSD)andandrogenreceptor(AR)wereisolatedfromspottedseabass(Lateolabraxmaculatus).ThemRNAabundancesofCYP17Iand3β-HSDincreasedfromstageⅡtostageⅤwithasignificantincreaseatstageⅤ,andthehighestabundanceofARmRNAwasdetectedatstageⅢintesticulardevelopmentcycle.CYP17I,3β-HSDandARtranscriptswereobviouslyabundantinsteroidogenesistissuessuchastestis,brain,headkidneyamongothers.StrongandpositivesignalswereobservedmainlyininterstitialcellregionsofL.maculatustestisasweremeasuredwithinsituhybridizationmethod.SignificantincreasesofCYP17Iand3β-HSDtranscriptsweredetectedafter12–48hhCG(humanchorionicgonadotropin)andGnRHa(gonadotropin-releasinghormoneanalogue)treatments.However,anoppositerelationshipwasfoundforARintestisatthesametime.Inaddition,decreasingtrendsofCYP17Iand3β-HSDmRNAwereobservedintestisofL.maculatusinfreshwatergroup(FW)fromday2today6,andmRNAabundanceofARincreasedinbrackishwater(BW)groupfromday4today8.Thesefindingsrevealedthatthesethreesteroidsynthesisgenesareimportfortesticulardevelopment,hormoneandsalinitytreatment,andprovidedalsoaninsightintothemechanismofreproductiveendocrineofL.maculatus.
简介:1概况世界气象组织(WMO)农业气象学委员会(CAgM)第17次届会[组第1次会议于2018年11月29—30日在瑞士日内瓦WMO总部召开。参加会议人员包括本次届会的主席RogerStone(澳大利亚)、副主席OrivaldoBrunini(巴西)、主席顾问FedericaRossi(意大利),第1至第4重点领域组的组长和联合组长,即重点领域1(农业天气和气候服务)的组长YvetteEveringham(澳大利亚)和联合组长KyurangKim(韩国)、重点领域2(农业气象学的研究与技术开发)的组长HarlanShannon(美国)和联合组长LorenaFerreira(阿根廷)、重点领域3(农业气象风险管理)的组长周文胜(中国)和联合组长MeriemAlaouri(摩洛哥)、重点领域4(沟通、教育与能力)的组长AdamouSitta(尼日尔)和联合组长ElenaMateescu(罗马尼亚),以及WMO农业气象处的处长RobertStefanski、科学总监JoseCamacho和秘书JenniferMunqai.