简介:Generally,ROSareregardedashostdefensemoleculesthatarereleasedbyneutrophilstodestroyexogenouspathogenssuchasbacteriaandtoactassecondarymessengersinsignaltransduction.However,increasedproductionofROSisalsoinvolvedininducingpathophysiologicalchangessuchasapoptosis,cellcycledisruption,andnecrosis.Forthisreason,inductionofROS-mediateddamageincancercellsbypharmacologicalagentsthateitherpromoteROSgenerationordisablethecellularantioxidantsystemisconsideredapotentialtherapeuticstrategyforpreferentiallykillingcancercells[1].
简介:Pt/WO3/CnanocompositeswithparallelWO3nanorodsweresynthesizedandappliedasthecathodecatalystforprotonexchangemembranefuelcells(PEMFCs).Electrochemicalresultsandsinglecelltestsshowthatanenhancedactivityfortheoxygenreductionreaction(ORR)isobtainedforthePt/WO3/CcatalystcomparedwithPt/C.ThehighercatalyticactivitymightbeascribedtotheimprovedPtdispersionwithsmallerparticlesizes.ThePt/WO3/Ccatalystalsoexhibitsagoodelectrochemicalstabilityunderpotentialcycling.Thus,thePt/WO3/CcatalystcanbeusedasapotentialPEMFCcathodecatalyst.
简介:摘要目的为了提高本院检验科尿液分析的准确性,有效性,比较分析GEB-600尿干化学分析仪(简称GEB-600),及IQ200尿沉渣仪(简称IQ200)及手工法显微镜镜检(简称手工法)检测尿液红细胞、白细胞结果。方法随机抽取本院住院患者304份新鲜的尿液标本,分别用GEB-600,IQ200及手工法进行检测,比较分析检测结果。结果GEB-600,IQ200及手工法检测RBC的阳性率分别为21.4%、19.4%、18.8%;WBC的阳性率分别为21.7%、22.7%、22.0%。经χ2检验RBC、WBC的检出率差异无统计学意义(P>0.05)。结论有效的结合尿液干化学分析仪,尿沉渣分析仪及手工显微镜镜检对尿液进行检测,可以快速,准确的为临床提供有效可靠地检验结果。
简介:AIM:ToinvestigatetheroleoftheoverexpressionofB7-H3inapoptosisincolorectalcancercelllinesandtheunderlyingmolecularmechanisms.METHODS:SW620cellsthathighlyoverexpressedB7-H3(SW620-B7-H3-EGFP)andHCT8cellsstablytransfectedwithB7-H3shRNA(HCT8-shB7-H3)werepreviouslyconstructedinourlaboratory.CellstransfectedwithpIRES2-EGFPwereusedasnegativecontrols(SW620-NCandHCT8-NC).Real-timePCRandwesternblottinganalysiswereusedtodetectthemRNAandproteinexpressionsoftheapoptosisregulatorproteinsBcl-2,Bcl-xlandBax.Acellproliferationassaywasusedtoevaluatethesurvivalrateanddrugsensitivityofthecells.Theeffectofdrugresistancewasdetectedbyacellcycleassay.Activecaspase-3westernblottingwasusedtoreflecttheanti-apoptoticabilityofcells.WesternblottingwasalsoperformedtodeterminetheexpressionofproteinsassociatedwiththeJak2-STAT3signalingpathwayandtheapoptosisregulatorproteinsafterthetreatmentwithAG490,aJak2specificinhibitor,inB7-H3overexpressingcells.ThedatawereanalyzedbyGraphPadPrism6usinganon-pairedt-test.RESULTS:WhetherbyoverexpressioninSW620cellsordownregulationinHCT8,B7-H3significantlyaffectedtheexpressionofanti-andpro-apoptoticproteins,atboththetranscriptionalandtranslationallevels,comparedwiththenegativecontrol(P<0.05).AcellproliferationassayrevealedthatB7-H3overexpressionincreasedthedrugresistanceofcellsandresultedinahighersurvivalrate(P<0.05).Inaddition,theresultsofcellcycleandactivecaspase-3westernblottingprovedthatB7-H3overexpressioninhibitedapoptosisincolorectalcancercelllines(P<0.05).B7-H3overexpressionimprovedJak2andSTAT3phosphorylationand,inturn,increasedtheexpressionofthedownstreamanti-apoptoticproteinsB-cellCLL/lymphoma2(Bcl-2)andBcl-xl,basedonwesternblotting(P<0.05).AftertreatingB7-H3overexpressingcellswiththeJak2-specificinhibitorAG490,thephosphorylationofJak2andSTAT3,andtheexp
简介:Er3+/Yb3+codopedzincateBaGd2ZnO5phosphorsweresynthesizedviaatraditionalsolidstatereaction.ThecrystalstructureandphasepuritywerecheckedbymeansofX-raydiffraction(XRD),andtheresultsshowedthatpurephaseBaGd2ZnO5phosphorswithvariousEr3+,Yb3+concentrationswereobtained.TheinfluenceofEr3+andYb3+dopingconcentrationsonthegreenandredupconversionemissionswasstudied.Itwasfoundthatbothgreenandredupconversionemissionsunder980nmexcitationweretwo-photonprocessesindependentfromtherareearthdopingconcentrations.However,theupconversionluminescenceintensitiesgreatlydependedontherareearthdopingconcentration.Furthermore,thepopulationprocessesofupconversionluminescenceandthequenchingmechanismswereanalyzed.Thetemperature-dependentgreenupconversionluminescencewasstudied,andthetemperaturequenchingprocessoftwogreenupconversionemissionswasmodeled.Thethermalquenchingprocessesofthegreenupconversionemissionscouldbewellexplainedbythemodelweproposed.