简介:TheGCanalysisoffattyacidoffourspeciesofmarinemicroalgaeculturedatdifferenttemperatureshowsthatthetemperaturehasdistincteffectsonthecompositionandunsaturateddegreeoffattyacidofmicroalgae.ThepercentagesofTPUFAinTFAandthenumberofmeandouble-bondoffattyacidsotIsochrysisgalbana,DunaliellasalinaandPhaeodactylumtricornutumdecreasewiththeascendingtemperature,whilethoseofTMUFAandTSFAincreaseundertheconditionsofthepresentexperiment.ThecontentofTPUFAandthenumberofmeandouble-bondsofChlorellasp.firstdecreaseandthenincrease,withtheminimumoccurringat20℃.
简介:盐水的侵入在最近的年里的珍珠河河口是严肃的。为到河口的盐水的运动的更好的理解和分析,三维的有限卷的沿海的海洋模型(FVCOM)被做在三个半月形的条件下面在珍珠河河口模仿咸度侵入到四条西方的水路。与测量并且模仿的根均方差(RMSE)和在多重地点,数字水铺平的结果表演,咸度和流动的水水平和咸度的吝啬的绝对百分比错误,速度与测量数据一致。使用FVCOM模仿盐水侵入在可接受、可行西方珀尔河的四条水路。与数字数据,时间和沿着Modao水路的盐水的侵入的空间运动模式被分析。咸度轮廓在春天潮前在35天期间通常到达它的山峰。咸度层化在时期是更明显的退潮比那在升起的潮是否在春天或小潮潮。盐流动思考进河口的盐的变化,和变化规则接近咸度侵入的规则。
简介:Westudiedtheeffectsofnitrogen(N)andphosphorus(P)supplyonFv/Fm(maximalquantumyieldofphotosystemII)inthealgaeChaetocerosdebilis,Dicrateriainornata,PlatymonassubcordiformisandHeterosigmaakashiwotodeterminethesensitivityofFv/FmtoP-limitationofthefourspecies.ObviousdecreaseofFv/FmvaluewasmonitoredinperiodsofP-depletion,thedecreaseshowingdifferentmagnitudesamongalgalspecies.AmorecleardecreaseofFv/FminPlatymonassubcordiformisandHet-erosigmaakashiwowasobserved,comparedwiththatinChaetocerosdebilisandDicrateriainornata.AftertheresupplyofP,theindexFv/Fmofthefourspeciesallrecoveredquicklyin24h.CelldivisionwasmaintainedandchlorophyllcontentcontinuedtoincreaseuntiltheendoftheexperimentinPlatymonassubcordiformisandHeterosigmaakashiwo,whileinChaetocerosdebilisandDicrateriainornata,thedivisionstoppedinthelaterperiodwhenthegrowthoforganismscameintothestationaryphase.ThemostobviousrespondofchlorophyllcontenttoP-additionswasobservedinHet-erosigmaakashiwoculturein24h.ThesensitivityofFv/Fmtonutrientlimitationsisdifferentamongalgalspecies,andmuchshouldbedonetoimprovetheapplicationofthisindex.
简介:TheantineoplasticactivityofpolysaccharidewasinvestigatedinStichopuschlorontus,Isostichopusbadionotus,StichopushorrensandHolothurialessonimassin.CrudepolysaccharidewaspreparedwithenzymehydrolyzationmethodandpurifiedbyanionexchangechromatographyusingDEAE-sepharosefastflowcolumn.TheeffectofpolysaccharideoncellsapoptosisofSiHaandU87wasexaminedwithcellcountingkit-8colorimetrymethod.Westernblottingwasusedtoanalyzerelatedproteinsofcellularapoptosisincludingp53andBcl-2.Resultsshowedthatthereweretwomaincomponentsineachseacucumberpolysaccharide,whichcouldbeeluteddownby1.0mol/LNaClsolution.ThefourtypesofpolysaccharideinthesecondcomponentwerenamedasSC-2,IB-2,HLM-2andSH-2,respectively.Theywereusedforcomparingtheantineoplasticactivity.ResultsshowedthatSC-2,IB-2,HLM-2andSH-2couldpromoteapoptosisofU87andSiHacells.SH-2andHLM-2wereselectedforthesubsequentexperimenttoexploretheadditionaleffectofU87andSiHacells,TheproteinexpressionsofBcl-2andp53decreasedconsiderablywiththeincreaseofpolysaccharideconcentrationinU87cells.InSiHacells,proteinexpressionsofBcl-2andhighdosagegroupofp53decreasedsignificantly,whereasnoobviousdecreasewasobservedinothergroups.ThepolysaccharidesaremoreeffectiveinpromotingapoptosisofU87andSiHacellsfromS.horrensandH.lessonimassinthanfromtherestspecies.
简介:Anumericalwavemodelbasedonthemodifiedfour-ordernonlinearSchrdinger(NLS)equationindeepwaterisdevelopedtosimulatefreakwaves.Astandardsplit-step,pseudo-spectralmethodisusedtosolveNLSequation.Thevalidationofthemodelisfirstlyverified,andthenthesimulationoffreakwavesisperformedbychangingsidebandconditions.Resultsshowthatfreakwavesentirelyconsistentwiththedefinitionintheevolutionofwavetrainsareobtained.Thepossibleoccurrencemechanismoffreakwavesisdiscussedandtherelevantcharacteristicsarealsoanalyzed.
简介:放大碎片长度多型性(AFLP)技术被用来在马尼拉蚌(Ruditapesphilippinarum)的四张自然人口分析基因差异,在中国的四个海区域分布式,我。e.,Bohai海,Huanghai海,华东海和华南海。264AFLPloci在195individuals被分析并且揭示了基因差异的高水平。多态的loci的百分比从92.13%~96.06%并且香农“s信息索引从0.2568~0.2756。由分析分子的变化(AMOVA),有在Qingdao和另外的三个海区域的人口之间的基因区别的高水平,这被发现。由Nei的聚合分析“spairwisedistances由地理起源组织了标本,除了Qingdao的人口。一个结论能被得出在在中国和一些不同差别的马尼拉蚌的四张自然人口有高基因的差异,这存在在之中并且在四张人口之间。结果也显示人的耕作活动将在Qingdao的人口的基因结构上有大影响。
简介:Horizontalandverticaldistributionsofδ18Oandδ13Cwereinvestigatedinshellsoffourplanktonicforaminiferalspecies,Globigerinoidesruber,Globigerinoidessacculifer,PulleniatinaobliquiloculataandNeogloboquedrinadutertrei,fromatotalof62core-topsedimentsamplesfromtheIndonesianthroughflowregion.Resultswerecomparedtomodernhydrologicconditionsinordertoexplorepotentialofproxiesinreconstructingfluvialdischargeandupperoceanwatercolumncharacteristicsinthisregion.Ourresultsshowthat,intheMakassarStrait,bothofdepletedδ18Oandδ13Cofthesefourspecieswerelinkedtofreshwaterinput.IntheBaliSea,however,depletedδ18Oandδ13Cforthesespeciesmaybeduetodifferentreasons.Depletedδ18Owasaresultoffreshwaterinputandaswellinfluencedbyalong-shorecurrentswhiledepletedδ13CwasmorelikelyduetotheJava-Sumatraupwelling.Comparisonofshellδ18OrecordsandhydrographicdataofWorldOceanAtlas2005suggeststhatG.ruberandG.sacculifercalcifywithinthemixed-layer,respectivelyat0–50mand20–75mwaterdepth,andP.obliquiloculataandN.dutertreiwithintheupperthermocline,bothat75–125mwaterdepth.N.dutertreicalcifiesatslightlydeeperwaterdepththanP.obliquiloculatadoes.Ingeneral,δ13CvaluesofbothG.ruberandG.sacculiferarelargerthanthoseofP.obliquiloculataandN.dutertreiatallsites,possiblyrelatedtodepthhabitatsofthesespeciesandverticaldistributionofnutrientsintheIndonesianthroughflowregion.
简介:Intertidalmarinemacroalgaeexperienceperiodicalexposuresduringlowtideduetotheirzonationaldistribution.ThedurationofsuchemersionleadstodifferentexposuresoftheplantstolightandaerialCO2,whichthenaffectthephysiologyofthemtodifferentextents.TheecophysiologicalresponsestolightandCO2wereinvestigatedduringemersionintworedalgaeGloiopeltisfurcataandGigartinaintermedia,andtwobrownalgaePetaloniafasciaandSargassumhemiphyllum,growingalongtheShantoucoastofChina.Thelight-saturatednetphotosynthesisinG.furcataandP.fasciashowedanincreasefollowedbyslightlydesiccation,whereasthatinG.intermediaandS.hemiphyllumexhibitedacontinuousdecreasewithwaterloss.Inaddition,theupper-zonatedG.furcataandP.fascia,exhibitedhigherphotosynthetictolerancetodesiccationandrequiredhigherlightleveltosaturatetheirphotosynthesisthanthelower-zonatedG.intermediaandS.hemiphyllum.Desiccationhadlesseffectondarkrespirationinthesefouralgaecomparedwithphotosynthesis.Thelight-saturatednetphotosynthesisincreasedwithincreasedCO2concentrations,beingsaturatedatCO2concentrationshigherthanthepresentatmosphericlevelinG.furcata,G.intermediaandS.hemiphyllumduringemersion.ItwasevidentthattherelativeenhancementofphotosynthesisbyelevatedCO2inthosethreealgaeincreased,thoughtheabsolutevaluesofphotosyntheticenhancementowingtoCO2increasewerereducedwhenthedesiccationstatusesbecamemoresevere.However,inthecaseofdesiccatedP.fascia(waterlossbeinggreaterthan20%),lightsaturatednetphotosynthesiswassaturatedwithcurrentambientatmosphericCO2level.ItisproposedthatincreasingatmosphericCO2willenhancethedailyphotosyntheticproductioninintertidalmacroalgaebyvariedextentsthatwererelatedtothespeciesandzonation.