简介:【摘要】目的:回顾1例超低出生体重儿腹膜透析治疗急性肾功能衰竭的护理,旨在为此类患儿的护理提供参考。方法:回顾性分析本科收治的1例超低出生体重儿急性肾功能衰竭行腹膜透析的临床资料。结果:该例患儿接受透析治疗5天,肾功能逐渐恢复正常,顺利拔除腹透管。总结:腹膜透析是治疗新生儿急性肾功能衰竭重要手段,全面、细心的护理干预,可使腹膜透析顺利进行,并有效减少严重并发症的发生。
简介:TheaimofthisstudyistoinvestigatecyclinE,pl6inkdaandki67aspossiblediagnosticbiomarkersforcervicalpreneoplasiausingcervicalexfoliated-cellspecimens,andevaluatethesignificanceforscreeningpatientsathighriskofdevelopingcervicalcarcinoma.TheexpressionofcyclinE,pl6inkdaandki67wasexaminatedin78cervicalexfoliatedepithelialspecimensdiagnosedasatypicalsquamouscellsofundeterminedsignificance(ASCUS)(12cases),cervicalintraepithelialneoplasia(CIN)oftype1(17cases),CIN2_3(38cases)andinvasivecarcinoma(11cases)usingimmunohistochemicalanalysis,andsimultaneously,theDNAstatusofhumanpapillomavims(HPY)type16/18wasdetectedbypolymerasechainreaction(PCR)usingtypespecificprimers,cyclinE,pl6inkdaandki67werealloverexpressedinCINsandinvasivecarcinoma,comparedwithlittleexpressioninASCUS(P<0.005).OverexpressionofcyclinEwasobservedinCIN1(94.1%,X^2=21.16,P<0.01),andp16inkdaandki67wereoverexpressedininvasivecarcinoma(100%and90.9%respectively).Thedegreeofpl6inkdaandki67expressioncorrelatedwellwiththatofepitheliallesions(P<0.005).HPV16/18infectionwasassessedinC1Nsandinvasivecarcinomasamples,andrevealedasignificantrelationshipwiththedegreeofcervicalepitheliallession.Theexpressionlevelofpl6inkdaandki67seemedmorecloselyassociatedwithHPVI6infectionthanthatofcyclinE(rs=1.0vsrs=0.4).Only1caseinCINIanddcasesinCIN2-3ofHPV18positivesamplesweredetected.Thereforenostatisticalsignificancewasfoundbystatisticalanalysis.OverexpressionofcyclinE,pl6inkdaandki67inCINsandinvasivecarcinomacellsdemonstratesthepotentialuseofcyclinE,pl6inkdaandki67asdiagnosticbiomarkersforHPV-relatedcervicalneoplasticlesions.Inaddition,thistechniquecanbeusedforscreeningpatientsathighriskofdevelopingcervicalcarcinoma.
简介:ToconstructanexpressionvectorcontainingtheE1glycoproteingeneofrubellavirusforthestudyontheeffectofmutationoftheE1geneglycoproteinandtheanalysisofphylogeneticdifferencesofsequences,thegeneencodingtheE1envelopeglycoproteinwasamplifiedfromrubellavirus,JinanstrainJR23,byRT-PCRandligatedintoPMD-18Tvector.TheclonesthatcarriedtheE1genewereidentifiedafteramprselectionandanalysisofrestrictionenzymedigestion.AftersequencingthisgenewasanalyzedbyDanstarandWinstarprograms,andthemapofphylogenetictreewasdrawn.ThecloneofE1glycoproteinwasthusconstructed.ItwasfoundthatthesequencedifferencesbetweenJR23strainandtheTCRBstrainfromJapanandthosebetweenJR23strainandThomasstrainofEnglandwererathersmallwithdifferencevaluesof0.9%and1.2%respectively.YetthosebetweenJR23strainandBRD2strainfromBeijingandthosebetweenJR23strainandXG379strainfromHongKongwerecomparativelylargerwithdifferencevaluesof7.6%and7.3%respectively.ThesequenceofJR23strainwithotherstrainswaslessthan3%excepttheNCstrain(3.7%).ItconcludesthattheconstructionofE1glycoproteingeneoffersanapproachtostudytherelationshipbetweenstructuresandfunctionsofE1geneanditsgeneproducts.Inthephylogenetictree,itshowsthattherearesignificantdifferencesinthesequencesofrubellavirusisolatedinChina,andthismightbehelpfultodevelopaneffectivesubunitvaccine.
简介:Theimmuneefficiencyofarecombinantadenovirustype5withtype35fibercontainingHIV-1gaggene(rAd5/F35-mod.gag)wasinvestigatedinBALB/cmice,inwhichtherAd5/F35-mod.gagwasfirstlyidentifiedwithPCR,thentransfectedto293cellsandtheinvitroexpressionlevelofGagproteinwasdeterminedbyWesternblottingandindirectimmuno-fluorescentassay.MicewereimmunizedwithintramuscularinjectionsofrAd5/F35-mod.gag,rAd5-mod.gagorDNAandwereboostedafter3weeks.Totesttheeffectofpre-existinganti-viralimmunityonimmunization,micewerealsoinjectedwithAd5-GFPvectorandthenimmunized4and7weekslaterwithAd5/F35-mod.gagvector.TheP24-specificIgGantibodyinseraofimmunizedmicewasdeterminedbyELISAandthespecificcytotoxicTlymphocyte(CTL)responsewasassayedbyintracellularcytokinestaining.ItwasdemonstratedthattherAd5/F35-mod.gagvectorcouldexpressefficientlytheHIVGagproteinin293cellsinvitroandinducestrongHIV-specificimmuneresponsesinvivo.ThestrongestCTLandserumIgGresponseoccurredwhenmicewereimmunizedtwicewithinjectionofrAd5/F35alone,buttheanti-Ad5antibodyafterprimaryinfectionwithadenoviruscouldinhibitthespecificimmuneresponsesinducedbyrAd5/F35vector.ItisconcludedthatsingleimmunizationwithrecombinantadenovirusrAd5/F35-mod.gagcaninducespecificCTLandserumIgGantibodyresponsesinmice,buttheimmunogenicityofrAd5/F35iscomparablyweakerthanthatofrAd5.
简介:【摘要】:痛经,为妇科常见病。本病通常在经行前后发作,表现为下腹部的坠痛、腰酸,甚者出现疼痛发射至大腿内侧、痉挛、呕吐、昏厥。严重影响女性正常的生活工作学习。痛经在临床上可分为原发性和继发性,本篇主要阐述原发性。现代医学的治疗方法多为止痛药物类治疗,具有依赖性,停药后常常出现复发,且存在着副作用。导师金影副教授结合多年临床经验,将此病的致病重点归据与瘀,治疗原则重点也在于化瘀,往往效果甚佳。今取验案1例,供广大专业从事者分享借鉴。
简介:[摘要 ] 总结 1例妊娠合并精神分裂患者的护理经验、护理体会。本文介绍了妊娠合并精神分裂症患者术前术后安全护理,做好病情观察,保持患者情绪稳定并合理进行母乳喂养,重视家庭支持系统等方面的护理体会,无并发症。
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简介:【摘要】 全皮下心脏复律除颤仪植入术( ICD )是一种能终止危及生命的心律失常情况的多功能电子装置,能自动检测及治疗心源性疾病,如心动过速、心室颤动及心动过缓等,是目前预防心脏性猝死最有效的方法。但随着 ICD 在临床使用的增多,其虽然降低了心源性猝死的发生率,但术后并发症情况也难以忽视,受到临床的越来越多的关注。因此,采取有效护理措施在保证治疗效果其减少并发症方面有者积极的作用。
简介:摘要: 目的 探讨关节镜辅助下联合 MIPPO技术治疗青年胫骨平台骨折的手术方法和术后疗效。本次报告 1例 31岁青年右侧胫骨平台骨折病例,患者因年龄及对膝关节术后恢复要求较高,入院后查膝关节 MRI为 SchatzkerⅡ型 ,且关节面塌陷严重程度较小,术前检查无明显手术禁忌症,决定行关节镜辅助下联合 MIPPO技术治疗【1】,结果手术时间较短,术中出血明显较少,手术切口较小且未造成关节内软组织进一步破坏。术后给以膝关节支具固定,术后 3天顺利出院。