简介:目的:研究心肌细胞条件培养液(cardiomyocyteconditionedmedium,CMCM)对K562细胞及NCI-H2452细胞增殖的影响,探索CMCM抑癌作用是否有肿瘤特异性,并探究其理化性质。方法:原代培养Wistar乳大鼠心肌细胞,以顺铂(DDP)作为阳性对照,CCK8法分析CMCM对K562细胞及人间皮瘤细胞NCI-H2452以及正常肝细胞HL7702体外增殖的影响。随后将CMCM采取不同比例稀释,探究稀释浓度与抑瘤作用间的关系。将CMCM采用胰酶消化,予以不同温度处理,以探究CMCM的理化性质。结果:K562细胞及NCI-H2452细胞分别与CMCM共同培养后,细胞存活率明显下降,与对照组相比差异均具有统计学意义(P<0.05),而对正常细胞存活率无明显影响(P>0.05)。经胰酶消化及热处理后的CMCM活性仍然存在,CMCM对K562细胞的抑制作用具有时间及浓度依赖性。结论:CMCM可抑制K562细胞及NCI-H2452细胞的增殖,CMCM不能被蛋白酶所破坏,对热也稳定,抑制作用具有时间及浓度依赖性。
简介:目的探究血清糖类抗原125(CA125)、神经元特异性烯醇化酶(NSE)及24h尿3-甲氧基-4羟基扁桃酸(VMA)在小儿神经母细胞瘤诊治中的应用价值。方法选取58例神经母细胞瘤患儿,采用电化学发光免疫分析法测定了患儿的血清CA125、NSE水平,采用高效液相色谱仪测定了患儿的24h尿VMA水平。对不同临床分期、近期疗效神经母细胞瘤患儿的血清CA125、NSE及24h尿VMA水平进行比较。结果58例神经母细胞瘤患儿的血清CA125、NSE及24h尿VMA阳性率分别为96.6%、81.0%、89.7%;临床分期为Ⅳ期的神经母细胞瘤患儿的血清CA125、NSE及24h尿VMA水平均明显高于临床分期为Ⅱ~Ⅲ期的患儿(P﹤0.01);治疗有效组患儿的血清CA125、NSE及24h尿VMA水平均明显低于治疗无效组(P﹤0.01)。结论血清CA125、NSE及24h尿VMA水平可以在一定程度上评估小儿神经母细胞瘤患儿的病情和治疗效果。
简介:Objective:Stromalinteractionmolecule1(STIM1)overexpressionhasbeenreportedtoplayanimportantroleinprogressionofseveralcancers.However,themechanismofSTIM1overexpressionanditsrelationshipwithhypoxiainpancreaticductaladenocarcinoma(PDAC)remainsunclear.Methods:STIM1andHIF-1αexpressionwastestedusingimmunohistochemistryintissuemicroarray(TMA)includingpancreaticcancerandmatchednormalpancreatictissues,andtheirrelationshipswithclinicopathologicalparameterswerestatisticallyanalyzed.q-PCR,Westernblot,ChIP,andluciferaseassaywereemployedto030analyzetranscriptionalregulationbetweenHIF-1αandSTIM1inpancreaticcancerPANC-1cells.Results:BothSTIM1andHIF-1αshowedhigherpositiveratesandup-regulatedexpressionincancertissuescomparedtothatofnormaltissues(P<0.05).TheKaplan–MeiermethodrevealedthathigherHIF-1αandSTIM1expressionlevelsweresignificantlycorrelatedwithdecreaseddisease-freesurvival(P=0.025andP=0.029,respectively).TheexpressionofHIF-1αshowedasignificantpositivecorrelationwiththatofSTIM1incancertissues(rs=0.3343,P=0.0011)andpancreaticcancercelllines.Furthermore,ChIPandluciferaseassaysconfirmedthatHIF-1αboundtotheSTIM1promoterandregulateditsexpressioninPANC-1cells.Conclusions:Inhypoxiamicroenvironment,up-regulatedexpressionofSTIM1mediatedbyHIF-1αpromotesPDACprogression.HIF-1αandSTIM1arepotentialprognosticmarkersand/ortherapeutictargetsforPDACtreatment.
简介:神经纤毛蛋白-1(Neuropilin-1)最早是作为轴突导向分子collapsin/semaphorin的受体被发现,在神经发育过程中引导轴突选择正确途径以成功到达靶区,与此同时又可作为血管内皮生长因子受体-2(VEGFR-2)的共受体表达于血管内皮细胞,在血管新生中发挥作用。近来的研究表明NRP-1可通过依赖于VEGF和独立于VEGF的方式参与调节肿瘤血管新生,在肿瘤生长转移中发挥作用。