简介：Background:Themainobjectiveofthisstudywastoexaminetheclimaticsensitivityoftheradialgrowthresponseof13easternwhitepine(PinusstrobusL.)provenancesplantedatseventestsitesthroughoutthenorthernpartofthespecies’nativedistributionineasternNorthAmerica.Methods:Thetestsites(i.e.,Wabeno,Wisconsin,USA;Manistique,Michigan,USA;PineRiver,Michigan,USA;Newaygo,Michigan,USA;TurkeyPoint,Ontario,Canada;Ganaraska,Ontario,Canada;andOrono,Maine,USA)examinedinthisstudywerepartofarange-widewhitepineprovenancetrialestablishedintheearly1960sintheeasternUnitedStatesandCanada.Principalcomponentsanalysis(PCA)wasusedtoexaminethemainmodesofvariation[first(PC1)andsecond(PC2)principalcomponentaxes]inthestandardizedradialgrowthindicesoftheprovenancesateachtestsite.TheyearscoresforPC1andPC2wereexaminedinrelationtoanarrayoftestsiteclimatevariablesusingmultipleregressionanalysistoexaminethecommonalityofgrowthresponseacrossallprovenancestotheclimateofeachtestsite.ProvenanceloadingsonPC1andPC2werecorrelatedwithgeographicparameters(i.e.,latitude,longitude,elevation)andasuiteofbiophysicalparametersassociatedwithprovenanceoriginlocation.Results:TheamountofvariationinradialgrowthexplainedbyPC1andPC2rangedfrom43.4%to89.6%.Dendroclimaticmodelsrevealedthatwhitepineradialgrowthresponsestoclimatewerecomplexanddifferedamongsites.Thekeydendroclimaticrelationshipsobservedincludedsensitivitytohightemperatureinwinterandsummer,coldtemperatureinthespringandfal(i.e.,beginningandendofthegrowingseason),summermoisturestress,potentialsensitivitytostorminduceddamageinspringandfal,andbothpositiveandnegativeeffectsofhigherwintersnowfal.Separationoftheloadingsofprovenancesonprincipalcomponentaxeswasmainlyassociatedwithtemperature-relatedbioclimaticparametersofprovenanceoriginat5ofth

简介：Apotexperimentwasconductedtoinvestigatetheeffectsofnitrogencontent[N1(nofertilizer),N2(0.15g?kg–1),andN3(0.3g?kg–1)]onthegrowthandthehydrauliccharacteristicsofpeachseedlingsunderdifferentsoilmoistureconditions(W1,W2andW3,inwhichthesoilwatercontentwas45%to55%,60%to70%,and75%to80%ofthefieldwatercapacity,respectively)byusingaspecializedhighpressureflowmeterwitharootchamberandacoupling,whichwasconnectedtoplantorgans.Leafareaandleafhydraulicconductivity(KL)increasedsignificantlyintheseedlingsbecauseofincreasedsoilmoistureandNcontent.KLincreasedwithleafarea.AlinearcorrelationwasdocumentedbetweenKLandleafarea.KLwashigherinthemorningandbegantodeclinesharplyafter16:00,atwhichKLdeclinedafteraninitialincrease.SoilmoistureandNcontentenhancedshoot(Ks)androot(Kr)hydraulicconductivities,therebyimprovingthelowsoilmoistureconditiontoalargeextent.KsandKroftheseedlingswerereducedby32%and27%respectivelyinN1,andby14.7%and9.4%,respectivelyinN2,andbothinW1,comparedwiththecontroltreatment.N3hadnosignificanteffectonKsandKrundersimilarconditions.LinearnegativecorrelationswereobservedbetweenKrandtheexcisedrootdiameteraswellasbetweenKsandtheshootstemdiameter.Theshoot-to-rootratioincreasedwithincreaseinNcontent.Theshoot-to-rootratioinN3wasincreasedby14.37%,comparedwithN1inW1aswellasby12%and4.39%inW2andW3,respectively.KnowledgeoftheeffectsofsoilmoistureandNfertilizeronhydrauliccharacteristicsandgrowthisimportant.Ourresultsprovidebasicguidelinesfortheimplementationofwater-savingirrigationandfertilizationmanagementofnurserystock.

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简介：ThaijasminericeKDML105isconsumedaroundtheworld.BKOS,PKOSandTKOSarenewcultivarsproducedfromlow-energyionbeaminductioninKDML105.ThepurposeofthisstudyistocomparethemorphologyandanatomybetweenKDML105andthethreenewcultivars.Seedsofthefourcultivarsweregerminatedandgrowninpotsuntilfloweringphase.Theplants'organswereobservedandthelengthsofculms,ligules,leavesandpaniclesweremeasured.Leafsurfaceareawascalculatedandnumbersofroots,spikeletsandtillerswerecounted.BKOSandPKOShadsignificantlyshorterculmsthanKDML105andTKOS.ThelargestleafareawasfoundinKDML105followedbyTKOS,BKOSandPKOS,respectively.NumbersofrootsandtillersinBKOSandTKOSweresignificantlyfewerthanthoseinKDML105andPKOS.ThenumberofspikeletsperplantinBKOSwasthelowestamongallcultivars.Foranatomicalcomparison,crosssectionsofculmsandrootswereobserved.Allplantshadasimilararrangementoftissues,butthenumberandsizeofcellsweredifferent.Furthermore,longitudinalsectionsofculmsshowedthatthelengthsofepidermalandparenchymacellsweredirectlyrelatedwiththelengthoftheculm.Tocomparetheleaves,bothstomataandepidermalcellswerecountedandthelengthsoftheguardcellsweremeasured.ThelengthsofguardcellsofBKOSandPKOSwereshorter,butthestomataldensityandthestomatalindexweresignificantlygreaterthanthoseofKDML105.ForTKOS,thoughthelengthofguardcellswasshorterthanthatinKDML105,thedifferencewasnotsignificant.However,thestomataldensityandstomatalindexweresignificantlyhigherthanthoseinKDML105.

简介：以植物乳杆菌P-8（Lactobacillusplantarumsubsp.plantarumP-8）亚油酸异构酶为研究对象,通过http：//swissmodel.expasy.org/,http：//smart.embl-heidelberg.de/和vecterNTI等在线工具与软件,对亚油酸异构酶进行生物信息学分析并预测与酶活性相关的位点,预测亚油酸异构酶的3个氨基酸可能为第68位甘氨酸、第107位精氨酸和第172位组氨酸。设计1对含突变的引物,以重组质粒pQE30-LAI为模板,利用PCR介导的定点突变技术构建突变体。经序列比对表明,成功构建了突变体G68A（甘氨酸突变为丙氨酸）、R107L（精氨酸突变为亮氨酸）和H172P（组氨酸突变为脯氨酸）,为进一步研究LAI的结构和功能奠定了基础。

简介：为明确480g/L毒死蜱乳油对苹果绵蚜及桃小食心虫的防治效果,特选取虫害发生较重的苹果园进行了田间药效试验。结果表明,480g/L毒死蜱乳油1500倍液药后21d对苹果棉蚜的防效最高可达89.70%,药后15d对桃小食心虫的防效最高可达94.34%,防效较好且对果树安全。

简介：我们使用了联合代理人改进在小麦稻草粒子和UF树脂之间的结合的能力的silane，并且调查了表面性质(wettability和表面粗糙)和用与由联合代理人内容和稻草/白杨木头粒子比率的silane影响了的白杨木头相结合的结合UF的小麦稻草(TriticumaestivumL.)做的particleboard的坚硬。我们在0，5%和10%的三个层次在稻草的四不同比率生产了一个分层的particleboard面板到白杨木头粒子(0%，15%，30%和45%小麦稻草)和silane联合代理人内容。粗糙大小，平均粗糙(R),平均数peak-to-valley高度(Rz),和根平均数平方粗糙(Rq)被使用跟踪技术的一支好尖笔在unsanded样品上测量。我们由使用连接到一个数字照相机和计算机系统的角度计获得了接触角度大小。包含白杨粒子的更大的数量的板与控制样品相比有优异坚硬并且有更低的wettability。与有的silane的更高的数量做的面板降低R<潜水艇class=“a-plus-plus”>q值。

简介：本研究利用以0—153为父本和SGK9708为母本构建的196个陆地棉重组自交系（F6：8）为材料对棉籽油分含量和蛋白质含量进行了遗传分析和QTL定位。通过四个环境下的群体材料的棉籽油分含量和蛋白质含量分析表明棉籽油分含量和蛋白质含量均为典型数量性状，其中棉籽油分含量存在超低亲本的超亲分离，而其蛋白质含量呈现超高亲本的超亲分离。相关性分析显示棉籽油分含量和蛋白质含量呈极显著负相关，同步提高两者在棉籽的的含量较为困难。基于包含186个标记，总长827．84cM，标记间平均距离4．45cM，覆盖棉花基因组18．6％的遗传连锁图谱，应用WinQTLcart2．5软件对四个环境下的棉籽油分含量和蛋白质含量进行了QTL定位，共检测到8个油分含量QTLs，解释表型变异5．42％～13．15％，其中稳定的QTL1个。4个蛋白质含量QTLs，解释表型变异4．35％～14．93％。本研究结果可为进行陆地棉种子营养品质性状的分子遗传改良奠定基础。

简介：我们用辣椒（Capsicumannuum）栽培种（已导入了灯笼椒CapsicumchinenseL^3基因）的种内F2代群体（2016株）和种间F2代群体（3391株）（由灯笼椒与Capsicumfrutescence杂交产生）对灯笼椒抗烟草花叶病毒属病毒的L^3基因进行定位。通过L^3基因抗性紧密相关的AFLP分子标记的BAC文库的分析，揭示出番茄抗病同源基因12的存在。通过简并PCR技术，对来自35株不同辣椒的同源基因12的部分或全部编码序列进行克隆，且在种间组合中产生了17个遗传标记。图谱显示：L^3基因位于12同源基因标记IH1—04和BAC—end标记189D23M中间，L^3基因定位于包含两个不同BAC重叠群的区内，这两个不同的BAC重叠群分别由4个和1个无性系组成。DNA纤维荧光原位杂交揭示这两个重叠群被约30kb隔开。DNA纤维荧光原位杂交结果和BAC无性系的Southern杂交表明在高度重复序列中富集包含L^3基因位点的区。Southern杂交表明两个BAC重叠群包含多于十个的12同源基因拷贝体。相反，对于种间F2代群体，，重组后代没有结合位点，在种内F2代群体中，该结合位点存在于两个不同的BAC重叠群内，这两个不同的BAC重叠群分别由7个和2个无性系组成。而且，两个群体间结合位点分配的不同表明在含有L基因位点的区域连锁不平衡。