简介：AbstractBackground:Hematopoietic stem cells (HSCs) have the ability to differentiate into all subsets of blood cells and self-renew. Large tumor suppressor 1 (LATS1) and large tumor suppressor 2 (LATS2) kinases are essential for cell cycle regulation, organism fitness, genome integrity, and cancer prevention. Here, we investigated whether Lats1 and Lats2 are critical for the maintenance of the self-renewal and quiescence capacities of HSCs in mice.Methods:Quantitative reverse transcription-polymerase chain reaction was used to determine the expression levels of Lats1 and Lats2 in subsets of progenitor cells and mature bone marrow cells. A clustered regularly interspaced short palindromic repeats system was used to generate Lats1 or Lats2 knockout mice. Complete blood cell counts were used to compare the absolute number of white blood cells, lymphocytes, monocytes, neutrophils, and platelets between Lats1 or Lats2 heterozygotes and littermates. Flow cytometry was used to assess the size of hematopoietic progenitor cells (HPCs) and HSC pools in Lats1 or Lats2 heterozygotes and littermates. The comparison between the two groups was analyzed using Student’s t test.Results:Lats1 and Lats2 were widely expressed in hematopoietic cells with higher expression levels in primitive hematopoietic cells than in mature cells. Lats1 or Lats2 knockout mice were generated, with the homozygotes showing embryonic lethality. The size of the HPC and HSC pools in Lats1 (HPC: wild-type [WT] vs. heterozygote, 220,426.77 ± 54,384.796 vs. 221,149.4 ± 42,688.29, P = 0.988; HSC: WT vs. heterozygote, 2498.932 ± 347.856 vs. 3249.763 ± 370.412, P = 0.105) or Lats2 (HPC: WT vs. heterozygote, 425,540.52 ± 99,721.86 vs. 467,127.8 ± 89,574.48, P = 0.527; HSC: WT vs. heterozygote, 4760.545 ± 1518.01 vs. 5327.437 ± 873.297, P = 0.502) heterozygotes were not impaired. Moreover, the depletion of Lats1 or Lats2 did not affect the overall survival of the heterozygotes (Lats1: P = 0.654; Lats2: P = 0.152).Conclusion:These results indicate that a single allele of Lats1 or Lats2 may be sufficient for normal hematopoiesis.

简介：

简介：我们试图与BPH在在正常、肥胖的病人之间的征兆的良性的prostatic增生(BPH)的医疗的功效调查差别；肥胖被任何一个身体团索引(BMI)或腰圆周(WC)决定。在这12星期的未来的观察研究，175个病人的一个总数变老≥；有国际前列腺症状分数(IPSS)≥的40年；12点和前列腺体积≥；20 ；ml有希望地被注册。病人根据BMI或WC被划分成二个组。病人们收到了doxazosin在4 的剂量的胃肠的治疗学的系统(GITS)；mg一次每为12个星期的天。从在IPSS的基线的变化，最大的尿流动率(Q最大),虚空以后的剩余体积，生活(QoL)的质量分数和不利事件(AE)被分析。175个注册病人，132完成了学习。57个病人有BMI>；23 ；kgm−2,和43有的WC>；90 ；厘米。肥胖的病人由WC>代表了；90 ；厘米或BMI≥；23 ；kgm−2在基线与非肥胖的病人相比有显著地更大的前列腺体积。全部的IPSS在WC>是显著地更高的；90 ；与WC≤相比的厘米组；90 ；厘米组。全部的IPSS断然与前列腺体积(P=0.031)和WC(P=0.045)被相关。所有组在12个星期在全部的IPSS和QoL显示出重要改进。然而，全部的IPSS的改进在high-BMI和high-WC组是更大的。最经常的AE是头昏(n=13)，并且它在肥胖的BPH病人是显著地更低的。肥胖与增加的前列腺体积和更低的尿道症状被联系。Alpha-blockers看起来为控制症状有效，特别在肥胖的人。

简介：AbstractBackground:Normal tension glaucoma (NTG) is a less pressure-dependent type of glaucoma with characteristic optic neuropathy. Recently, the biomechanical mechanism has been thought to account for glaucomatous optic neuropathy to some degree. We intended to compare dynamic corneal response parameters (DCRs) among patients with primary open-angle glaucoma with normal tension or hypertension and controls. The correlations between DCRs and known risk factors for glaucoma were also analyzed.Methods:In this cross-sectional study, 49 NTG subjects, 45 hypertension glaucoma (HTG) subjects, and 50 control subjects were enrolled. We compared the differences in DCRs using corneal visualization Scheimpflug technology among the NTG, HTG, and control groups. We also analyzed the correlations between DCRs and known risk factors for glaucoma (eg, central corneal thickness [CCT], intraocular pressure [IOP], etc).Results:The maximum inverse concave radius (NTG: 0.18 [0.17, 0.20] mm-1; control: 0.17 [0.16, 0.18] mm-1; P= 0.033), deformation amplitude ratio of 2 mm (DAR 2 mm, NTG: 4.87 [4.33, 5.39]; control: 4.37 [4.07, 4.88]; P < 0.001), and DAR 1 mm (NTG: 1.62 [1.58, 1.65]; control: 1.58 [1.54, 1.61]; P < 0.001) were significantly higher in NTG than in the controls. The integrated radius (IR, NTG: 8.40 ± 1.07 mm-1; HTG: 7.64 ± 1.31 mm-1; P = 0.026) and DAR 2 mm (NTG: 4.87 [4.33, 5.39]; HTG: 4.44 [4.12, 5.02]; P < 0.007) were significantly higher, whereas the stiffness parameter at the first applanation (SP-A1, NTG: 91.23 [77.45, 107.45]; HTG: 102.36 [85.77, 125.12]; P = 0.007) was lower in NTG than in HTG. There were no significant differences in the DCRs between HTG and control groups (P > 0.05). In the univariate and multivariate analyses, some of the DCRs, such as IR, were negatively correlated with CCT and IOP, whereas SP-A1 was positively correlated with CCT and IOP.Conclusions:The cornea was more deformable in NTG than in HTG or controls. There were no significant differences in corneal deformability between HTG and controls. The cornea was more deformable with the thinner cornea and lower IOP.

简介：BackgoundEssentialhypertension(EH)isapolygenicinheritabledisease,generallyknownasacombinedresultofgeneticandenvironmentalelements.ItispossiblethatIR、AngiotensinⅡ(AngⅡ)andNOplayimportantrolesinthepathogenesisofEH.MethodsSixtynormalsubjectswithafamilyhistoryofEH,aged30to40yearsold,wererecruitedandrandomizedintotwogroups:30withoneparentand30withbothparentswithEH,and30subjectswithoutfamilyhistoryofEHascontrols.TheplasmalevelofNOwasdeterminedbyelectrophotometerwhiletheplasmaleveloffastinsulin(FINS)andAngⅡweredeterminedbyradioimmuno-assay,andinsulinresistanceindex(IRI)wascalculated.Results①TheplasmalevelsofFINS,AngⅡ,NOincreasedsignificantlyinstudygroupscomparedwiththoseinthecontrolgroup(P<0.01),whiletherewerenodifferencesinthelevelsofAngII,NObetweenthetwostudygroups(P>0.05).②TheplasmalevelofAngIIwerepositivelycorrelatedwiththatofNO(r=0.378,P<0.01).ConclusionsThehigherlevelsofIRandplasmaAngIIandNOexistbeforethedevelopmentofEHinnormaloffspringwithafamilyhistoryofEH,andmaybetheyareinitialagentsinthepathogenesisofEH.ItindicatesthatthepeoplewithIRandhighlevelsofplasmaAngⅡandNOwithafamilyhistoryofEHareathigherrisktodevelopEH.

简介：Thebrainasasystemwithgraduallydecreasingresourcesmaximizesitschancesbyreorganizingneuralnetworkstoensureefficientperformance.Auditoryevent-relatedpotentialswererecordedin28healthyvolunteerscomprising14youngand14elderlysubjectsinauditorydiscriminationmotortask(lowfrequencytone-righthandmovementandhighfrequencytone-lefthandmovement).Theamplitudesofthesensoryevent-relatedpotentialcomponents(N1,P2)weremorepronouncedwithincreasingageforeithertoneandthiseffectforP2amplitudewasmorepronouncedinthefrontalregion.ThelatencyrelationshipofN1betweenthegroupswastone-dependent,whilethatofP2wastone-independentwithaprominentdelayintheelderlygroupoverallbrainregions.Theamplitudesofthecognitivecomponents(N2,P3)diminishedwithincreasingageandthehemisphericasymmetryofN2(butnotforP3)reducedwithincreasingage.ProlongedN2latencywithincreasingagewaswidespreadforeithertonewhilebetween-groupdifferenceinP3latencywastone-dependent.HighfrequencytonestimulationandmovementrequirementsleadtoP3delayintheelderlygroup.Theamplitudedifferenceofthesensorycomponentsbetweentheagegroupscouldbeduetoageneralgreateralertness,lessexpressedhabituation,ordeclineintheabilitytoretreatattentionalresourcesfromthestimuliintheelderlygroup.Withaging,aneuralcircuitreorganizationofthebrainactivityaffectsthecognitiveprocesses.Theapproachusedinthisstudyisusefulforanearlydiscriminationbetweennormalandpathologicalbrainagingforearlytreatmentofcognitivealterationsanddementia.

简介：

简介：WereportontheexperimentalrealizationofCerenkovsum-frequencygenerationacrossthematerialdispersioninaone-dimensional,periodicallypoledferroelectriccrystal.Threeschemesofsum-frequencygeneration,confinedonlyinthevicinityofdomainwallsandintheformofnonlinearCerenkovradiation,aredemonstratedinnormal,degenerated,andanomalous-dispersion-likeconfigurations.Weexploittheirphase-matchinggeometries,whichexhibitawholescenariooftheevolutionofCerenkovradiationvaryingwiththedispersionrelationshipamongtheinteractionwaves.Inaddition,twosetsofconicalsum-frequencygenerationwithdifferentradiusandcenteraredemonstrated,whichresultfromscatteringassistantphase-matchingprocesses.

简介：

简介：

简介：这份报纸地址关节风波浪导致了在正常状态和差错事件下面的近海的漂浮风汽轮机(OFWT)调节的一种半类型的动态回答。在这份报纸的分析及时被进行领域，用aero-hydro-servo-elastic模拟代码--快。由于唯一粘滞参考书系统的特征，实现在的原来的粘滞的抑制模型快被二次的代替获得精确俘获粘滞效果。模拟盒子在静止的水里包含免费腐烂的运动，面对常规波浪稳定运动并且象处于有或没有风的运作的海状态的动态反应一样弯屈。模拟也包括盒子因为快片在紧急情况以后投球导致的短暂回答关机。在许多刺激下面的站台运动，本地结构的负担和典型绳索行紧张力量的特征被获得并且调查。

简介：

简介：Inthisstudy,wesoughttoelucidatetheeffectsofmelatoninonlearningandmemoryaswellasapoptosisandexpressionoftheBaxorBcl-2proteinsinthesubgranularzoneofthedentategyrusinpinealectomizedrats.UsingtheMorriswatermazeandtheolfactorymemorytests,wefoundthattheaverageescapelatencyinpinealectomizedratswasclearlyincreasedcomparedwithsham-operatedrats.Moreover,theaverageescapelatencyinthemelatonin-treatedandpinealectomizedratswaslongerthanthatinthesham-operatedratsandshorterthanthatinthepinealectomizedanduntreatedrats.Immunohistochemistryandterminal-deoxynucleoitidyltransferasemediatednickendlabeling(TUNEL)showedthattherewerefewerBaximmunoreactivecellsandTUNEL-positive(apoptotic)cellsbutmoreBcl-2immunoreactivecellsinthemelatonin-treatedratscomparedwiththepinealectomizedrats.Thesham-operatedratsshowednumbersofthesecellssimilartothemelatonin-treatedrats.TheseexperimentalfindingsdemonstratethatmelatonintreatmentmayreduceabnormalapoptosisbypromotinggeneexpressionofBaxandsuppressinggeneexpressionofBcl-2inthesubgranularzoneofthedentategyrusinpinealectomizedrats.Theseeffectsappeartoresultintheinhibitionofcellularapoptosisandtheimprovementofspatiallearningandmemoryinpinealectomizedrats.

简介：

简介：

简介：AssyriologyQIXiao,MATheReconstructionoftheArchiveofUr-Kununna,theScribeofAnimalCenterofUr-IIIDynasty(ulgi43ii-Ibbi-Sin2iv)(Supervisor:WuYuhong)

简介：~~

简介：Assyriology,SupervisorWUYuhong,AACUDProject,2011LIXueyan,Ph.D.TheReconstructionoftheArchiveofNasa,theGeneralManagerandFattener(kuru

简介：~~

简介：Thesol-gelprocessofcitricacidchelatingwithmetalcationsforthesynthesisofnormalspinelLiMn2O4andthereactionmechanismwereinvestigatedbymeansofXRD,IR,TG-DTA,andSEM.Theresultsshowthatatthebeginninglithiumcitrateandchelatecompoundofcitricacidwithmanganeseionsformed,andthenwithheatingtheesterificationandcondensationreactionsoccuredbetweenthemandglycol.TheproductsobtainedarepolymersinwhichmetalcationsaredistributedhomogeneouslyonatomicscalethatensurehighreactivitytocationsofLi+andMn2+.Firingthegelpreparedbythisprocess,thelatticediffusionsofsolidreactantionscausedbynon-homogeneityofreactantsareeliminatedandavoided.At400℃phase-pureLiMn2O4withnanometerscalecrystallizationhavingprecisestoichiometryandperfectcrystallizationcanbeobtained.Themodelofchelatecoordinateofdouble-moleculebetweencitricacidandMn2+inthegelnetworkisproposed.ItisimportantforexplainingthedispersionstateofMn2+andtheformationprocessofgelbythismodel.