摘要
Tostudytheinsecticidalactivityandtoxicitymechanismofspinosad,weassayedbioactivityofspinosadbyleafmembranemethodanditseffectsontheactivitiesofdetoxifyingandprotectiveenzymesinMalacosomaneustriatestacealarvaebyspectrophotometry.Theresultsshowedthatspinosadhadanextremelyhightoxicityagainstthe4thand5thinstarlarvae.TheglutathioneS-transfer(GST)activityin4thand5thinstarlarvaewasfirstlyinhibited,theninduced,andfinallyinhibited,whilethemixed-functionaloxidase(MFO)activitywasinhibitedandthenenhanced.Theinducedeffectoncarboxylesterase(CarE),theinducedandinhibitedeffectonacetylcholinesterase(AchE)andperoxidase(POD),andthecomplicatedeffectsonsuperoxidedismutase(SOD)andCatalase(CAT)weredeterminedin4thinstarlarvaewithspinosadtreatment.However,theobviousinhibitioneffectswerefoundontheactivitiesofCarE,AchE,SODandPODinthe5thinstarlarvaewhereasthecatalase(CAT)activitywasinhibited,thenincreased,andinhibitedfinally.Therefore,spinosadcouldeffectivelydisruptandinterferenotonlythedetoxifyingandprotectiveenzymesbutalsonormalphysiologicalmetabolismofM.neustriatestaceaandshowextremelyhightoxicityagainstthispest.
出版日期
2012年03月13日（中国期刊网平台首次上网日期，不代表论文的发表时间）
