摘要
ThisstudyexaminedtheeffectofsalinityontheexpressionofNa+/K+-ATPase(NKA)α-subunitandvacuolar-typeH+-ATPase(V-ATPase)β-subunitgeneinthegillofLitopenaeusvannamei.Semi-quantitativereversetranscription-polymerasechainreaction(RT-PCR)assayshowedthattheexpressionofNKAα-subunitandV-ATPaseβ-subunitgenewassignificantlyinfluencedbysalinity.ItwasfoundthattheNKAactivitysignificantlyvariedwithsalinityintimeanddosedependentmanner;whereastheV-ATPaseactivitydidnot.TheabundanceofNKAα-subunitgenetranscriptincreasedrapidlywhenthesalinitydecreasedfrom26bto21,andslowlywhenthesalinitydecreasedfrom26to31withinthefirst24h.Whenthesalinitydecreasedfrom26to21,thetranscriptionofNKAα-subunitgeneingillepitheliumwashigherat12hthanthatat0h,whichwasconsistentwiththeresultofimmunoblottingassayofNKAα-subunit.Inaddition,salinityhadasignificanttime-anddose-dependenteffectontheconcentrationofbiogenicaminesinbothhemolymphandgill.Ascomparedtootherparameters,theconcentrationofdopamine(DA)and5-hydroxytryptamine(5-HT)variedindifferentpatternswhenthesalinitydecreasedfrom26to21orincreasedfrom26to31,suggestingthatDAand5-HTplayeddifferentregulatoryrolesinosmoticadaptionandmodulationofshrimpwhensalinityvaries.
出版日期
2014年03月13日（中国期刊网平台首次上网日期，不代表论文的发表时间）
