Identification of γ—aminobutyric acid transporter (GAT1) on the rat sperm

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摘要 SomerecentstudiesindicatedthatGABAergicsystemisinvolvedinmammalianspermacrosomereaction(AR),butdirestevidencepertainingtotheexpressionofgatlinmammalianspermisnotyetdemonstrated.Inthisstudy,weevaluatedthepresenceof67kDaGAT1proteinandmRNAinrattestisbyWesternblottingandreversetranscription-polymerasechainreaction.Meanwhile,immunohistochemicalandimmunofluorescentanalysesalsoidentifiedGAT1proteinontheelongatedspermatidandsperm.Theseresultsindicatedthatrattestisisanovelsiteofgatlexpression.FurtherstudiesshouldbetakentoexploretheroleofGAT1proteinonspermacrosomereaction.
机构地区 不详
出处 《细胞研究:英文版》 2000年1期
出版日期 2000年01月11日(中国期刊网平台首次上网日期,不代表论文的发表时间)
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