Identification and Characterization of Genes Responsible for Drought Tolerance in Rice Mediated by Pseudomonas fluorescens-中国期刊网

摘要 Droughtisoneofthemajorabioticstresseswhichadverselyaffectcropplantslimitinggrowthandyieldpotential.Structuralandfunctionalcharacterizationofdroughtstress-inducedgeneshascontributedtoabetterunderstandingofhowplantsrespondandadapttothedroughtstress.Inthepresentstudy,differentialdisplaytechniquewasemployedtostudythegeneexpressionofriceplantsatthereproductivestagethatweresubjectedtodroughtstressbywithholdingwater,Pseudomonasfluorescensstrain(Pf1)treatedplantssubjectedfordroughtstressbywithholdingwaterandcontrol(well-watered).DifferentiallyexpressedcDNAsofsixgenes(COX1,PKDP,bZIP1,AP2-EREBP,Hsp20andCOC1)wereidentified,clonedandsequenced.Real-timeqPCRanalysisshowedthatallthesixgeneswereupregulatedindrought-stressedplantstreatedwithPf1.ThisrevealedthattheremarkableinfluenceofPf1colonizationleadstodroughttoleranceatthereproductivestage.TheseresultsshowedthathighlevelsofgeneexpressioninplantslackingadequatewatercanberemarkablyinfluencedbyPf1colonization,whichmightbeakeyelementforinducedsystemictolerancebymicrobes.

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Identification and Characterization of Genes Responsible for Drought Tolerance in Rice Mediated by Pseudomonas fluorescens

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Identification and Characterization of Genes Responsible for Drought Tolerance in Rice Mediated by Pseudomonas fluorescens

来源期刊

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