摘要
ActivinAisakindofpre-inflammatoryfactorthatbelongstothetransforminggrowthfactor-β(TGF-β)superfamily.ToinvestigatetheeffectandmechanismofactivinAontheactivitiesofmousemacrophages,thesecretionofNOinthesupernatantofculturedmouseperitonealmacrophageswasexaminedbyNOassaykit,andtheexpressionofiNOS,ActRIIAandARIP2mRNAinmouseperitonealmacrophageswasanalyzedbyRT-PCR.TheresultsshowedthatactivinAstimulatedthesecretionofNOandtheexpressionofiNOSmRNAinnon-activatedmousemacrophagesinatime-anddose-dependentmanner.Incontrast,activinAinthesameconcentrationinhibitedthesecretionofNOinLPS-activatedmousemacrophagesinadose-dependentmanner.ActRIIAwashighlyexpressedonmacrophages,andactivinAupregulatedtheActRIIAmRNAexpressioninmacrophages.Anti-ActRIIAantibodycanblockthesecretionofNOfromthemacrophagesstimulatedbyactivinA.Furthermore,RT-PCRanalysisrevealedthatactivinAenhancedtheARIP2mRNAexpressioninmacrophages.TheseresultsindicatedthatActivinAmaybeaweakactivatorcomparedwithLPStomousemacrophages,andactivinAmaymodulatethesecretionofNOthroughActRIIA-ARIP2signalpathwayinmousemacrophages.
出版日期
2005年01月11日(中国期刊网平台首次上网日期,不代表论文的发表时间)