摘要
RapiddifferentialidentificationofMycobacteriumspeciesisessentialforeffectivediagnosisandmanagementofmycobacteriosis.Theaimofthisstudywastodevelopanovelmultiplexprobearraybasedonthe16S-23SrRNAgeneinternaltranscribedspacersequenceforthegenotypingofmycobacteriatothespecieslevel.Apairofprimersandasetofgenus-andspecies-specificprobesweredesignedfromtheconservedandpolymorphicregionsofthe16SrRNAgene,internaltranscribedspacer,and23SrRNAgenesequencesofmycobacteria.Weusedanovelmultiplexprobearrayforidentificationof266clinicalspecimensobtainedfrompatientswithmycobaterialinfection.Theresultsshowedthattheoverallspecificityandsensitivityofournovelprobearraywereboth100%forthegenus-specificprobeandMycobacteriumtuberculosiscomplex-specificprobe.Therewere79.3%(23/29)ofnontuberculousmycobacteriawhichcouldbeidentifiedtothespeciesleveldirectlyinthespecimensfromChina.SomeintraspeciesheterogeneityinM.avium,M.intracellulare,M.chelonaeandM.abscessuswasobserved.Withtheincreaseofsequencesofinternaltranscribedspacerandnumbersofwholemicrobialgenomes,andfurtheroptimizationofprobes,themultiplexprobearraywillbecomeapromisingtoolfortherapidandaccurateidentificationofmycobacteriainordinaryclinicallaboratories.
出版日期
2006年02月12日(中国期刊网平台首次上网日期,不代表论文的发表时间)