Theanatomycharacteristicsofcellwallandlignindistributionindifferentanatomicalregionsfor12-month-oldbamboospeciesPseudosasaamabiliswasinvestigated.Thelignindistributionwasstudiedbymeansofopticalmicroscopeandconfocallaserscanningmicroscopeandthecolorreaction.Inaddition,thedistributionofligninindifferenttissues(fiber,parenchymaandvessel)wasinvestigatedbyvisible-lightmicrospectrophotometrycoupledwiththeWiesnerandMaulereaction.Thecellwallsofdifferenttissueswerealllignifiedandtheligninwaswidelydistributedindifferenttissues,inwhichtherewasadifferencebetweentissuesandanatomicalregionsinlignincontent.Guaiacylligninandsyringylligninunitcouldbefoundincellwalloffiber,parenchymaandvesselthroughWiesnerandMaulereactionandtheabsorbancepeakofspectrum.Therewasnoregularvariationinlignincontentwithdifferentradiallocationofculmsanddifferentlocationinfiberstrands.Thelamellationofthefibercellwallwasgenerallyhadasalternatingbroadandnarrowlayerswithalternatinglowandhighconcentrationoflignin.