Theobjectiveofthispaperistodemonstratewhetherairplasmacanchangetheseedgerminationcharacteristics,seedlingemergence,aswellasbiochemicalreactivity,inAndrographispaniculata(A.paniculata)seedlingsbymodifyingtheseedcoatandfindingabeneficialtreatmentdose.Eighttreatmentdosesandonecontrolwereusedtoconductelectricalconductivitydetermination,agerminationtest,aseedlingemergencetestandabiochemicalassay.Theresultsshowedthatafterbeingtreatedwithairplasmaexcitedat5950Vfor10s,thepermeabilityoftheseedswasimprovedsignificantly,resultingintheaccelerationofseedgerminationandseedlingemergence.Inthemeantime,thecatalaseactivityandcatalaseisoenzymeexpressionwerealsoimproved,whilethemalondialdehydecontentintheseedlingswasdecreased(whichmeansgreatercounteractionwithenvironmentalstress).Afterbeingtreatedwith4250Vfor10sand5950Vfor20s,theseedgerminationwasenhanced,butwithoutanobviouschangeinseedlingemergence.However,aftertreatmentwith3400Vfor20sand5100Vfor10s,thepermeabilityoftheseedswasdecreased,resultinginadelayinseedlingemergence.TheseresultsindicatethatairplasmacanchangethephysiologicalandbiochemicalcharacteristicsofAndrographispaniculataseedsbymodifyingtheseedcoat,combinedwiththeeffectsoftheactiveplasmaspecies,andthatdifferenttreatingdoseshavedifferenteffects.
