Simplified heavy metal staining techniques demonstrated with Fast Plant leaf tissue

(整期优先)网络出版时间:1991-01-11
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FastPlant(Brassicarapa,Cruciferae)leaftissuefixedinglutaraldehyde-acroleinandpost-fixedinos-mium,wasexaminedforresponsetoseveraleasily-preparedheavymetalstains.Leadanduranium,separatelyandincombination,gavetypicalresultsacrossthespectrumofcellorganellets.Asasinglestainfollowingosmium,bismuthproducedimagesseeminglyequivalenttoleadanduranium.Phosphotungsticacidproducedverygoodmembranedelineationbutproducedawashed-outbackgroundimagesimilartothatfromleadstaining.Carbohydratecompoundswereespeciallyresponsivetoruthenium;thecytoplasmandthematrixofallorganelleswerealsostainedverywell.Theprocedureswerenomoredemandingthantraditionalstainingmethodsandmaybeeasilyusedinresearchandteaching.FastPlantmaterialsareareliable,quickandeasysourceoflivingmaterial.