Thedegradationpotentialofadiesel-degradingbacteria(HDMP1)isolatedfromtheoil-contaminatedmarineenvironmentwasexploredsystematicallybyanalyzingtheenvironmentalconditionsandsynergisticactionwithotherdiesel-degradingbacteria.TheresultindicatedthatHDMP1wasconfirmedasastrainofHalomonasbythemethodofstrainidentification.And,HDMP1showedgooddieseldegradationperformancewithadieseldegradationrateofupto79.59%after7days.ByanalyzingtheeffectofenvironmentalconditionsonHDMP1,thebestcarbonandnitrogensourceswerefoundtobelactoseandpeptone,respectively,atapHvalueof7.5andasalinityof4g/(100mL).Additionally,thesynergisticeffectofHDMP1combinedwithotherdiesel-degradingbacteriawasanalyzedbyorthogonalexperimentaldesign.TheinoculaofHDMP1,HDMP2,HDMP3andHDMB3wereoptimized,withthebestresultsequatingto0.4%,0.1%,0.4%and0.9%in100mLofMSM,respectively,whilethedegradationrateofdieselwasidentifiedtobe73.5%within5daysinthepresenceofoptimuminocula.