Exploring the Degradation Potential of Halomonas Bacteria from Oil-contaminated Marine Environment

(整期优先)网络出版时间:2018-04-14
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Thedegradationpotentialofadiesel-degradingbacteria(HDMP1)isolatedfromtheoil-contaminatedmarineenvironmentwasexploredsystematicallybyanalyzingtheenvironmentalconditionsandsynergisticactionwithotherdiesel-degradingbacteria.TheresultindicatedthatHDMP1wasconfirmedasastrainofHalomonasbythemethodofstrainidentification.And,HDMP1showedgooddieseldegradationperformancewithadieseldegradationrateofupto79.59%after7days.ByanalyzingtheeffectofenvironmentalconditionsonHDMP1,thebestcarbonandnitrogensourceswerefoundtobelactoseandpeptone,respectively,atapHvalueof7.5andasalinityof4g/(100mL).Additionally,thesynergisticeffectofHDMP1combinedwithotherdiesel-degradingbacteriawasanalyzedbyorthogonalexperimentaldesign.TheinoculaofHDMP1,HDMP2,HDMP3andHDMB3wereoptimized,withthebestresultsequatingto0.4%,0.1%,0.4%and0.9%in100mLofMSM,respectively,whilethedegradationrateofdieselwasidentifiedtobe73.5%within5daysinthepresenceofoptimuminocula.