Aim:Toconfirmthestabilityofexogenousgenesinthegenerationoftransgenicchickensusingejaculatedchickensperm,thedeoxyribonuclease(DNase)activitywasevaluatedintheseminalplasmaofejaculatedsemenandthestabilityofDNAwasexaminedbyaddinglipofectionreagents.Methods:APCRfragment(249bp)ofpEGFPN-1vectorwasusedastheDNAsubstrateandwasincubatedwiththeseminalplasmaat40℃for30min.Then,thewholereactionsolutionwassubjectedtoagarosegelelectrophoresisandtheDNAsizewasevaluatedunderUVlight.Results:TheDNAsubstratewascompletelydiminishedafterincubationwithseminalplasma.However,thesub-stratewasintactafterincubationwithheat-treatedseminalplasmaorincubationwithseminalplasmainthepresenceof0.5mmol/L-5mmol/LEDTA.Thesubstratewasstabilizedintheseminalplasmabytheadditionofcommerciallyavailablelipofectionreagents.Conclusion:TheDNaseactivityispresentintheseminalplasmaofejaculatedchickensemen.However,DNAisstableintheliposomal-DNAcomplex.(AsianJAndro12003Sep;5:213-216)
