ObjectToexploretileeffectoflipofectin-c-erbB2antisenseoligodeoxynucleotidesonradiosensitivityofhumanovariancancercellllne.MethodsTheexpressionofc-erbB2wasdetectedbymeansofRT-PCR,cellularresponsetoirradiationwasevaluatedbytilecolonyformingassay.ResultsLipofectin-c-erbB2antisenseoligodeoxynucleotides（AS-ODN)couldsuppresstheexpressionofc-erbB2,andsignificantlydecreasedthecolonyformingrateofhumanovariancancercellsafterionizingirradiation（P<0.01),whilenon-trean~entandthesenseoligodeoxynucleotides{S-ODN)groupsdidnotdecreaseontheradio-resistancelevelofSKOV3cellline{P>0.05).Condusionc-erbB2antisenseoligodeoxynueleotidessensitizedtheSKOV3toionizingirradiationthroughdecreasingtheexpressionofe-erbB2,whichmightbetheresultofthefactthatc-erbB2antisenseoligodeoxynueleotidesinhibittheeelluarsignaltransductionpathwayrelatingtotheradiation-resistantphenotype.
