AhepaticstimulatorsubstancefromSharkliver(sHSS)waspurifiedandcharacterized.Thepurificationprocedureincludedhomogenization,centrifugation,ultrafiltration,DEAE-sephadexA25chromatographyandFPLCmonoQchromatography.Bytheprocedure,thestimulatoryspecificactivityofsHSScouldbeincreasedto9000IU/mg,andwas750timesmorethanthecrudeextract.ThepurityofthefinalpurifiedfractionFTowhichretainedthemaximalactivityofsHSSwascheckedbypolyacrylamidegelelectrophoresisinthepresenceofsodiumdodecylsulfate(SDS-PAGE)andHighPerformanceGelFiltrationrespectively,andshowedonlyonebandoronepeak.Itsmolecularweightwasmeasuredtobe16973Dabymassspectrometry.ItsN-terminalsequencewasdeterminedtobeN-Met-Arg-Thr-Gln-Glu-His-Thr-Lys-Ser-ValbyPE-ABD491AProteinN-TerminalSequencingMeter.ItwasstableoverawiderangeofpHandtemperature.
