Analysis of the HLA-A,-B allele polymorphism in 5844 umbilical cord blood samples taken from Han population of Shandong province

ToinvestigatetheHLA-A,-BallelepolymorphisminHanpopulationofShandongprovinceandtoexplorethepossibilitytofindouttheHLA-A,-B-matchedcordblooddonorsforstemcelltransplantationtobeusedinotherareainChina,5844umbilicalcordbloodsamplesweretakenfromHanpopulationdonorsofShandongprovince,andassayedwithPCR-sequence-oligonucleotide(PCR-SSO)assay.InShandongHandonors,20allelesatHLA-Alocusand46allelesatHLA-Blocuscouldbedetectedasrevealedinthepresentstudy.Amongthe20allelesatHLA-Alocus,themostprevalentfiveallelesincludedA*02(0.3041),A*11(0.1443),A*24(0.1434),A*30(0.0975)andA*33(0.0859),while,thealleleswithlowergenefrequenciesincludedA*34(0.0006),A*25(0.0005),A*66(0.0005),A*74(0.0004)andA*(0.0001).Ofthe46HLA-Ballelesdetected,themostprevalentfivealleleswereB*13(0.1348),B*51(0.0713),B*62(0.0712),B*61(0.0676)andB*60(0.0642);whilealleleswithlowergenefrequenciesincludedB*77(0.0001),B*76(0.0002),B*47(0.0003),B*42(0.0003)andB*72(0.0004).IncomparisonwiththoseoftheotherHanpopulationinChina,theHLA-A,-BgenefrequenciesintheumbilicalcordbloodofShandongprovincepossessuniquedistributionfeaturesamongtheinvestigatedpopulationsfromvariousregionsofthesameraceorigin,andthedifferencesinvariousregionsofthesameracewerelessthanthoseamongthedifferentrace.ItisevidentthattheHLA-A,-BallelesoftheumbilicalcordbloodtakeninShangdongprovinceshowhighdegreeofpolymorphism,anditmightbepartofthoseofNorthernHanpopulationinChina.So,itisreasonableforpatientsofNorthernChinesetoreceiveHLAclassI-matchtransplantofcordbloodstemcellsfortissueandorgantransplantationfromShangdongumbilicalcordbloodbank.