Helicobacter pylori and EBV in gastric carcinomas:Methylation status and microsatellite instability

(整期优先)网络出版时间:2010-03-13
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AIM:ToverifythemethylationstatusofCDH1,DAPK,COX2,hMLH1andCDKN2AgenesandtoevaluatetheirassociationwithHelicobacterpylori(H.pylori)-cagA+andEpsteinBarrvirus(EBV)infectionsingastricadenocarcinomas.METHODS:Methylation-specificPCR(MSP)assaywasperformedin89primarygastriccarcinomas(intestinalanddiffusetypes).Microsatelliteinstability(MSI)analysiswasperformedusingtheBAT26primersetandPCRproductswereanalyzedwiththeABIPRISM3100GeneticAnalyzerusingG...