ToinvestigatetheexpressionlevelsofthreeDsbproteingenes,dsbB,dsbDanddsbG,atdifferenttimepointspostC.trachomatisinfection,mousefibroblast12cellswerechosentobeinfectedwithC.trachomatisserovarFstrainF/IC-Cal-13.C.trachomatiselementarybody(EB)-infected12cellswereharvestedimmediatelyafterEBattachmentontothecellsandevery4hourspostinfection(hpi)till44hpifortotalRNApreparation.RT-PCRassayswerethenemployedtoamplifycDNAwithprimerpairswhicharespecifictoC.trachomatisdsbgenesdsbB,dsbD,dsbGandtufArespectively.Therela-tiveexpressionlevelsofDsbproteingeneswereevaluatedascDNAratiosofgenedsbtogenetufA.OurresultsshowedthatthetranscriptionofdsbGstartedfrom12hpiandgraduallyincreasedtill44hpi.ThetranscriptionofdsbBanddsbDweredetectedat16hpiandreachedtheirpeaksat28hpiand24-28hpi,respectively.Moreover,therewasobvioustranscriptionofdsbBatthelaterstage(44hpi),butnonefordsbDatthistimepoint.WecametotheconclusionthattheexpressionlevelsofthethreeDsbproteingenesaredifferentduringthedevelopmentalcycleofC.trachomatist.Theymayplayaroleinmid-to-latestageofthedevelopmentalcycleofC.trachomatis.
