简介:Telomeraseactivitywasinhibitedinadoseandtime-dependentmannerwiththetreatmentofcisplatinfor24,48,or72hinaconcentrationrangedfrom0.8to50μMinBEL-7404humanhepatomacells.Therewerenochangesinexpressionpatternofthreetelomerasesubunits,itscatalyticreversetranscriptasesubunit(hTERT),itsRNAcomponent(hTR)ortheassociatedproteinsubunit(TP1),aftercisplatintreatedfor72hwithindicatedconcentrations.Meantelomerelengthsweredecreasedbythecisplatintreatment.CellgrowthinhibitionandcellcycleaccumulationinG2/Mphasewerefoundtobecorrelatedwithtelomeraseinhibitioninthepresentstudy,butpercentagesofcellapoptosisdidnotchangemarkedlyduringtheprocess.