简介：TheTheGreat-WestDykewithalengthof6.7km,thelargestseawallinChina,wascompletedinJune,1994.Thispaperintroducesstructureoptionsofnibblemoundseawallonsoftfoundationandanewtypeofarmourblock-hexagonmultiholeblock(HMB)andanewmethodoftreatmentforsoftfoundationbyusingunderwaterexplosion.ConstructionpracticeofsomeprojectsinChinashowsthatbyusingthismethodgoodeffectoftreatmentandmarkedeconomicbenefitcanbeachievedbyusingthismethod.

简介：为了学习简历，活跃代谢物由导出海绵的未耕作的共生者生产了，海绵Gelliodesgracilis的共生者的一个metagenomicDNA图书馆被构造。DNA的平均尺寸在图书馆插入是20kb。这个图书馆用纸圆盘assaying为抗菌素活动被屏蔽。二克隆对小球菌tetragenus显示了抗菌剂活动。这二克隆的代谢物通过HPLC被分析。结果证明他们的代谢物与主人E的那些相当不同。包含向量pHZ132的关口iDH5α和主人。这研究可以介绍一条新途径给探索简历海绵共生者的活跃代谢物。

简介：重建的向量pCANTAB5EE被插入包含了一个EcoRV识别序列进pCANTAB的34搁浅bpdouble的oligonucleotide获得5E。Whitespot症候群病毒(WSSV)染色体DNA被sonication碎裂主要在0的范围孤立碎片。8～2.0kb，当时，碎片与T4DNA聚合酶是钝结束的并且克隆5EE进pCANTAB的EcoRV地点。图书馆的主要recombinant克隆是随机的选择re-combinants的5.ColonyPCR显示出的3.0x10~inserts的尺寸是0.12～整个图书馆recombinant噬菌体感染了的1.77kb.AfterEscherichi--关口iHB2151房间，细胞外并且periplasmic摘录在PVDF膜上被扔执行点污点，usingpoly同种细胞的老鼠anti-VP24浆液，anti-WSV026浆液，anti-WSV063浆液，anti-WSV069浆液，anti-WSV112浆液，anti-WSV238浆液，anti-WSV303浆液和anti-VP26浆液结果证明显示图书馆能表示病毒的蛋白质。

简介：TheWei-LiuwatersupplyingsubmarinepipelineisatpresentthelongestoneofthekindinChina.Itisabout4kilometerslongfromWeihaiCitytoLiugongIslandinShandongprovince.Ittookonlyhalfayeartodesignandconstructit.Theprojectwasofahigheconomicefficiencyandsomepracticalexperienceswereachieved.Inthispaperageneralintroductiontothedesignandconstructionofthispipelineispresentedandsuchproblemsasthestabilityofthepipelineinverysoftclay,designofcurvedslideforlaunchingandestimationofdraggingforcesareexpounded.

简介：ThewildPorphyrayezoensiscollectedfromtheQingdaocoastwasusedtoprepareprotoplastsbyenzymedigestion.Thepurelinewasconstructedbycultivatingtheprotoplasts.The18SrDNAoftheP.yezoensispurelinewasclonedandsequenced.Sequenceanalysiswasexecutedforthissequenceandother22sequencesretrievedfromGenBank.Aphylogenetictreewasconstructedusingtheneighbor-joiningmethod.Theresultsrevealedahighdiversityof18SrDNAsequencesingenusPorphyraandtheconsiderablevariationof18SrDNAsequencesindifferentstrainsofthesamespeciesP.yezoensisandP.tenera.Significantdifferenceof18SrDNAsequencewasobservedbetweenP.yezoensisfromQingdao,China,andthetwostrainsofP.yezoensisfromJapan,butthethreestrainsofP.yezoensisformedastablecladeinthephylogenetictree.TheseresultsindicatethepossibilityofinterspeciesandintraspeciesdiscriminationofPorphyrausingthe18SrDNAsequences.