简介：Marineorganismsconstituteunlimitedresourceofbioactivesubstancesduetotheirhighbiodiversityandrepresentavaluablesourceofnewcompounds.Thisstudyoptimizedthealkali-extractionconditionsandantioxidantactivitiesofsolublepolysaccharidesfromthebodywallofSipunculusnudus.Theeffectsofsolid-liquidratio,extractionduration,extractiontemperature,andalkaliconcentrationontheyieldofS.nuduspolysaccharides(SNP)wereexamined,accordingtowhichtheoptimalcombinationofextractionparameterswasobtainedbyanorthogonaltest.TherelativeinfluencingimportanceofdifferentextractionparametersontheyieldofSNPfollowedtheorderassolid-liquidratio>extractiontemperature>alkaliconcentration>extractionduration.Thehighestextractionyield,1.98%,wasachievedunderanoptimalextractioncondition:temperature60℃,solid-liquidratio1:6gmL?1,duration5h,andalkali(NaOH)massfraction6%.TheinvitroantioxidantactivitiesexaminationshowedthatextractedSNPunderthisoptimizedconditionhadstrongpowerinreducingcertainhydroxylandsuperoxideradicalscavengingabilities.ThepromisingresultsshowedthatextractedSNPcouldbeapotentnaturalantioxidant.

简介：Multi-componentmooringsystemsbecomewidelyusedindeepwaterposition-keepingofdrillingandproductionplatforms.However,versatilematerialsmakeitdifficulttodesignappropriatemooringlinesmadeofseveralsegments.Basedoncatenaryequationsofamulti-componentmooringlineataspecificwaterdepth,thispaperestablishesaminimummodelfordesigningthiskindoflines.ThemodelissolvedbyGeneticAlgorithmandMulti-ObjectivePlanningrespectively.Themodelisverifiedbyitsapplicationtoapracticalmooringdesignassignment-aquasi-staticanalysisforalargesemi-submersible.Theoptimalresultisfinallyobtainedwiththeaidofdesigngraphs.

简介：在常规波浪的轮船漂浮状况是计算的。漂浮的轮船调节的新方程控制任何东西被向量操作的使用建议。这种形式是能与经常的系数用惩罚功能方法被解决的一个非线性的优化问题。并且解决的过程被两分加速。在解决的过程期间，轮船排水量和快活的中心被轮船表面的集成根据吃水线计算了。轮船表面用一个积聚的弦长度理论被描述以便决定排水量，快活中心和吃水线。在每个车站形成吃水线的风能被计算轮船表面和波浪表面的交叉发现。一个例子的结果显示这个方法准确、有效。漂浮能计算轮船在常规波浪调节以及简化计算并且改进计算效率和结果的精确。

简介：从有有势力反的马尾藻类海草tenerrimum(圣)的乙醇摘录的多酚过敏效果被学习通过列层析优化分离过程。三的吸附和解吸附作用特征广泛地使用了吸附物：宏多孔的树脂，硅石胶化，和polyvinylpolypyrrolidone(PVPP)，非常分别地被评估并且为多酚的准备分离的优化学习了。这些吸附物上的静态的操作显示出多孔的树脂有的那个宏在三吸附物之中的最好的吸附和解吸附作用能力。有包装的列也是的多孔的树脂进行了优化参数例如的宏的动态吸附和解吸附作用：与在方括号显示出的最佳的价值，摘录答案的集中(4次冲淡了)，pH价值(67)，吸附速度(3BVh−1,床volumes/per小时)，乙醇(80%)的集中，elution速度(3BVh−1)和elution体积(7BV)。那么优化的色析法的过程从粗略的多酚给了62.43%的纯净，提供简历的大规模准备的一个有希望的基础在在测试上面的进一步的可伸缩之上的活跃多酚。

简介：Waterqualityiscriticaltoensurethatmarineresourcesandtheenvironmentareutilizedinasustainablemanner.TheobjectiveofthisstudyisthereforetoinvestigatetheoptimumplacementofmarineenvironmentalmonitoringsitestomonitorwaterqualityinShanghai,China.Toimprovethemappingorestimationaccuracyoftheareaswithdifferentwaterqualitygrades,themonitoringsiteswerefixedintransitionbandsbetweenareasofdifferentgradesratherthaninotherpositions.Followingbidirectionaloptimizationmethod,first,18candidatesiteswereselectedbyfilteringoutspecificsitecategories.Second,threeofthesewere,inturn,eliminatedbecauseoftheruledefinedbythechangesintheareasofwaterqualitygradesandbythestandarddeviationoftheinterpolationerrorsofdissolvedinorganicnitrogen（DIN）andphosphate（PO_4-P）.Furthermore,indicatorkrigingwasemployedtodepictthetransitionbandsbetweendifferentwaterqualitygradeswhenevernewsamplingsiteswereadded.Thefouroptimizationprojectsofthenewlyaddedsitesrevealthat,alloptimizedsitesweredistributedinthetransitionbandsofdifferentwatergrades,andatthesametimeintheareaswherethehistoricalsitesweresparselydistributed.Newsiteswerealsofoundintheoverlapregionofdifferenttransitionbands.Additionalsiteswereespeciallyrequiredintheseregionstodiscriminatetheboundariesofdifferentwaterqualitygrades.Usingthebidirectionaloptimizationmethodofthemonitoringsites,theboundariesofdifferentwaterqualitygradescouldbedeterminedwithahigherprecision.Asaresult,theinterpolationerrorsofDINandPO_4-Pcouldtheoreticallydecrease.

简介：Toobtainthemaximumangiotensin-Iconvertingenzyme(ACE)inhibitoryactivity,theproteinhydrolysisconditionsofthejellyfishRhopilemahispidumwereoptimizedusingresponsesurfacemethodology(RSM).TrypsinwasselectedtoproduceR.hispidumproteinhydrolysates(RPH)withACEinhibitoryactivity.TheoptimalparametersforproducingproteinhydrolysateswiththehighestACEinhibitoryactivitywereasfollows:hydrolysistime5h,hydrolysistemperature50℃,andtheenzyme-to-substrateratio6%.Undertheseconditions,theACEinhibitoryrateofRPHcouldreach64.28%±5.72%.Inaddition,RPHcontainedhighlevelsofGly,Glu,Pro,Ala,AspandArg,withamolecularweightdistributionrangeof0.32–6.84kDa.ThefollowingthreenovelACEinhibitorypeptideswereisolatedandidentified:Ile-Gly-Glu-Thr-Gly-Pro,Gly-Ala-Thr-Gly-Pro-Ala-Gly-Tyr-ValandGly-Ala-Phe-Gly-Pro-Gly-Gly-Leu-Val-Gly-Arg-Pro.TheIC50valuesoftheACEinhibitoryactivityofthesethreepurifiedpeptideswere19.07,27.42and31.26μmolL^-1,respectively.TheseresultssuggestedthatproteinsandpeptidesisolatedfromR.hispidumcouldbeutilizedasantihypertensivefunctionalfoodsources.

简介：在复杂水流下面，管理海洋的电报的方程的运动复杂、非线性，并且电报配置和紧张的计算在一致或简单的水流下面与那些相比变得困难。获得数字结果，平常的Newton-Raphson重复经常被采用，但是它的稳定性取决于起始的猜测的答案到管理方程。改进数字计算的稳定性，建议的这份报纸分开了粒子群优化，变量在被分开成搜索空间的几个组，和尺寸被减少便于粒子群优化。经由分开的粒子群优化，这些管理非线性的方程能与任何起始的解决方案成功地被解决，并且数字计算的进程是很稳定的。为电报配置和在复杂水流下面的海洋的电报的紧张的计算，建议的分开的群粒子优化比另外的粒子群优化更有效。

简介：Harmfulalgalblooms(HABs)haveledtoextensiveecologicalandenvironmentalissuesandhugeeconomiclosses.VariousHABcontroltechniqueshavebeendeveloped,andbiologicalmethodshavebeenpaidmoreattention.Algicidalbacteriaisageneraldesignationforbacteriawhichinhibitalgalgrowthinadirectorindirectmanner,andkillordamagethealgalcells.AmetabolitewhichisstronglytoxictothedinoflagellateAlexandriumtamarensewasproducedbystrainDH46ofthealga-lysingbacteriumAlteromonassp.Thecultureconditionswereoptimizedusingasingle-factortestmethod.Factorsincludingcarbonsource,nitrogensource,temperature,initialpHvalue,rotationalspeedandsalinitywerestudied.Theresultsshowedthatthecultivationofthebacteriaat28℃and180rmin-1withinitialpH7and30saltcontcentrationfavoredboththecellgrowthandthelysingeffectofstrainDH46.TheoptimalmediumcompositionforstrainDH46wasdeterminedbymeansofuniformdesignexperimentation,andthemostimportantcomponentsinfluencingthecelldensityweretryptone,yeastextract,solublestarch,NaNO3andMgSO4.Whenthefollowingculturemediumwasused(tryptone14.0g,yeastextract1.63g,solublestarch5.0g,NaNO31.6g,MgSO42.3gin1L),thelargestbacterialdryweight(7.36gL-1)wasobtained,whichwasanenhancementof107%comparedtotheinitialmedium;andthealgallysisratewasashighas98.4%whichincreasednearly10%afteroptimization.

简介：Amutant(GQQ-M6)ofaSponge-Derivedstreptomycessp.GQQ-10obtainedbyUV-inducedmutationwasusedforproducingprodiginines(PGs).Singlefactorexperimentsandorthogonalarraydesign(OAD)methodswereemployedformediumoptimization.Inthesinglefactormethod,theeffectsofsolublestarch,glucose,soybeanflour,yeastextractandsodiumacetateonPGsproductionwereinvestigatedindividually.InthesubsequentOADexperiments,theconcentrationsofthese5keynutritionalcomponentscombinedwithsalinitywerefurtheradjusted.ThemutantstrainGQQ-M6gavea2.2-foldhigherPGsproductionthanthatoftheparentstrain;OADexperimentsofferedaPGsyieldof61mgL-1,whichwas10timeshigherthanthatoftheinitialGQQ-10strainundertheoriginalcultivationmode.

简介：OptimalstockingdensitieswereinvestigatedfortheseacucumberApostichopusjaponicusSelenkaunderfeed-supplementandnon-feed-supplementregimesinnetenclosuresfor333d.Substantialweightlossoccurredduringtheaestivationphase(AE).Decreasedgrowthrateswerealsoobservedduringthewinterphase(WT).Incontrast,seacucumbersshowedrapidgrowthduringthespring(SP)andautumn(AU)phases.Feedingregimesconsiderablyinfluencedthegrowthperformance,i.e.,seacucumbersgrewfasterunderfeed-supplementregimethanundernon-feed-supplementregime(P<0.05).Theaveragesurvivalratesofseacucumbersunderfeed-supplementregimewerehigherthanthoseundernon-feed-supplementregimeforboththeautumnphaseandspringphase,butthedifferenceswereonlysignificantforthelatterphase(P<0.05).ThefittedB-Ncurvesshowedthattheoptimalstockingdensities,intermsofnetproduction,were22.3ind.m-2forfeed-supplementregimeand14.1ind.m-2fornon-feed-supplementregime.

简介：Theproceduresofultrasonicextractionandclean-upwereoptimizedforthedeterminationofpolycyclicaromatichydrocarbons(PAHs)inmarinesediments.Sampleswereultrasonicallyextracted,andtheextractswerepurifiedwithaminiaturizedsilicagelchromatographiccolumnandanalyzedwithhighperformanceliquidchromatography(HPLC)withafluorescencedetector.Ultrasonicationwithmethanol-dichloromethane(2:1,v/v)mixturegavehigherextractionefficiencythanthatwithdichloromethane.Amongthethreeelutionsolventsusedinclean-upstep,dichloromethane-hexane(2:3,v/v)mixturewasthemostsatisfactory.Undertheoptimizedconditions,therecoveriesintherangeof54.82%to94.70%withRSDsof3.02%to23.22%foraspikedblank,andintherangeof61.20%to127.08%withRSDsof7.61%to26.93%foraspikedmatrix,wereobtainedforthe15PAHsstudied,whiletherecoveriesforaNISTstandardreferenceSRM1941bwereintherangeof50.79%to83.78%withRSDsof5.24%to21.38%.Thedetectionlimitswerebetween0.75ngL-1and10.99ngL-1fordifferentPAHs.AsamplefromtheJiaozhouBayareawasexaminedtotesttheestablishedmethods.